Background. Vertical root fractures (VRFs) can significantly reduce dental prognosis. Cone-beam computed tomography (CBCT) offers better visualization of VRF than conventional radiography. However, gutta-percha creates artifacts in cone-beam CT (CBCT) images and reduces the diagnosis quality. The purpose of this study was to evaluate the accuracy of CBCT in detection of VRF in presence and absence of gutta-percha in canals. Materials and Methods. In this cross-sectional study, 50 extracted mandibular premolars were selected. After preparing the access cavity, canals were instrumented using step-back method, and gutta-percha #40 was placed afterwards. The fractures were created using electromechanical universal testing machine on 25 teeth. The teeth were randomly placed in dry mandibular dental sockets, and the first CBCTs were taken. Then the gutta-percha was removed, and the second CBCTs were taken. Results. The results of Kappa coefficient between two observers in roots with and without gutta-percha were 0.644 and 0.830, respectively (p value ≤ 0.001). The sensitivity and specificity of VRF diagnosis in assessing gutta-percha filled canals were 32% and 68% for the first observer, respectively (Kappa
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