Unrestrained markerless trait stacking in Nannochloropsis gaditana through combined genome editing and marker recycling technologies [Applied Biological Sciences]

Robust molecular tool kits in model and industrial microalgae are key to efficient targeted manipulation of endogenous and foreign genes in the nuclear genome for basic research and, as importantly, for the development of algal strains to produce renewable products such as biofuels. While Cas9-mediated gene knockout has been demonstrated...

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