Machining protein microcrystals for structure determination by electron diffraction [Biophysics and Computational Biology]

We demonstrate that ion-beam milling of frozen, hydrated protein crystals to thin lamella preserves the crystal lattice to near-atomic resolution. This provides a vehicle for protein structure determination, bridging the crystal size gap between the nanometer scale of conventional electron diffraction and micron scale of synchrotron microfocus beamlines. The demonstration...

https://ift.tt/2D6n4tW