Mechanism of the E2 to E1 transition in Ca2+ pump revealed by crystal structures of gating residue mutants [Biochemistry]

Ca2+-ATPase of sarcoplasmic reticulum (SERCA1a) pumps two Ca2+ per ATP hydrolyzed from the cytoplasm and two or three protons in the opposite direction. In the E2 state, after transferring Ca2+ into the lumen of sarcoplasmic reticulum, all of the acidic residues that coordinate Ca2+ are thought to be protonated, including...

https://ift.tt/2PwowXq