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Medicine by Alexandros G.Sfakianakis,Anapafseos 5 Agios Nikolaos,Crete 72100,Greece,tel :00302841026182 & 00306932607174
Κυριακή 30 Οκτωβρίου 2016
A Simple and Efficient Approach to Construct Mutant Vaccinia Virus Vectors
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Subtyping of Campylobacter jejuni ssp. doylei Isolates Using Mass Spectrometry-based PhyloProteomics (MSPP)
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Ghostly Germs and Phantasmal Prokaryotes
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MALDI-TOF mass spectrometry for rapid diagnosis of postoperative endophthalmitis
Publication date: Available online 29 October 2016
Source:Journal of Proteomics
Author(s): Adriane Mailhac, Harmonie Durand, Sandrine Boisset, Danièle Maubon, Francois Berger, Max Maurin, Christophe Chiquet, Marie Bidart
This study describes an innovative strategy for rapid detection and identification of bacteria causing endophthalmitis, combining the use of an automated blood culture system with MALDI-TOF mass spectrometry methodology. Using this protocol, we could identify 96% of 45 bacterial strains isolated from vitreous samples collected in acute post-operative endophthalmitis patients.
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Unearthing Bulgakov's trace proteome from the Master i Margarita manuscript
Publication date: Available online 29 October 2016
Source:Journal of Proteomics
Author(s): Gleb Zilberstein, Uriel Maor, Emmanuil Baskin, Alfonsina D'Amato, Pier Giorgio Righetti
Ten pages, selected from a total of 127, of the last manuscript of Master i Margarita, written by Bulgakov in the last four years of his life, have been analysed in order to harvest and identify any trace proteome left on the margin by the novelist, in the hope of finding biomarkers of his fatal nephrotic syndrome. To that aim, we prepared a special ethyl-vinyl acetate film as binder of ground AG 501 Bio-Rad mix-bed strong cation/strong anion exchange resins for adsorbing any protein left on the margins of the pages via saliva and/or sweat. After eluting, digesting and interrogating the peptides by LC-MS/MS, we could identify three proteins, periostin, N-acetyl-β-glucosaminidase and nephrin, reported as biomarkers of renal pathologies. Additionally a further 29 unique gene products, of saliva and skin origin, have been identified, together with two bacterial proteins. The novel method here reported could be safely applied to any other research on manuscripts stored in public libraries and repositories of the World Cultural Heritage.SignificanceThe present manuscript aims at finding proteomics traces in a 75-year old manuscript in order to confirm the health state of the author. In the case of Bulgakov it was known that he died of renal disease, possibly leaving traces and/or biomarkers of this pathology on the margins of the pages analysed. Three proteins, stated to be biomarkers of nephrotic syndrome, could be identified. In order not to contaminate the manuscript pages with resin particles, we have devised a novel harvesting film, by which strong cation and anion exchangers are embedded in ethyl-vinyl acetate foils. It is felt that this technology could be safely applied to other specimens belonging to the Word Heritage.
Graphical abstract
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The Aotus nancymaae erythrocyte proteome and its importance for biomedical research
Publication date: Available online 29 October 2016
Source:Journal of Proteomics
Author(s): D.A. Moreno-Pérez, R. García-Valiente, N. Ibarrola, A. Muro, M.A. Patarroyo
The Aotus nancymaae species has been of great importance in researching the biology and pathogenesis of malaria, particularly for studying Plasmodium molecules for including them in effective vaccines against such microorganism. In spite of the forgoing, there has been no report to date describing the biology of parasite target cells in primates or their biomedical importance. This study was thus designed to analyse A. nancymaae erythrocyte protein composition using MS data collected during a previous study aimed at characterising the Plasmodium vivax proteome and published in the pertinent literature. Most peptides identified were similar to those belonging to 1189 Homo sapiens molecules; >95% of them had orthologues in New World primates. GO terms revealed a correlation between categories having the greatest amount of proteins and vital cell function. Integral membrane molecules were also identified which could be possible receptors facilitating interaction with Plasmodium species. The A. nancymaae erythrocyte proteome is described here for the first time, as a starting point for more in-depth/extensive studies. The data reported represents a source of invaluable information for laboratories interested in carrying out basic and applied biomedical investigation studies which involve using this primate.SignificanceAn understanding of the proteomics characteristics of A. nancymaae erythrocytes represents a fascinating area for research regarding the study of the pathogenesis of malaria since these are the main target for Plasmodium invasion. However, and even though Aotus is one of the non-human primate models considered most appropriate for biomedical research, knowledge of its proteome, particularly its erythrocytes, remains unknown. According to the above and bearing in mind the lack of information about the A. nancymaae species genome and transcriptome, this study involved a search for primate proteins for comparing their MS/MS spectra with the available information for Homo sapiens. The great similarity found between the primate's molecules and those for humans supported the use of the monkeys or their cells for continuing assays involved in studying malaria. Integral membrane receptors used by Plasmodium for invading cells were also found; this required timely characterisation for evaluating their therapeutic role. The list of erythrocyte protein composition reported here represents a useful source of basic knowledge for advancing biomedical investigation in this field.
Graphical abstract
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A COMPARATIVE STUDY OF RADIUM CONTENT AND RADON EXHALATION RATE FROM SOIL SAMPLES USING ACTIVE AND PASSIVE TECHNIQUES.
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A COMPARATIVE STUDY OF RADIUM CONTENT AND RADON EXHALATION RATE FROM SOIL SAMPLES USING ACTIVE AND PASSIVE TECHNIQUES.
Radiat Prot Dosimetry. 2016 Oct;171(2):254-256
Authors: Yadav M, Prasad M, Joshi V, Gusain GS, Ramola RC
Abstract
Soil is the most important factor affecting the radon level in the human living environments. It depends not only on uranium and thorium contents but also on the physical and chemical properties of the soil. In this paper, the measurements of radium content and mass exhalation rate of radon from the soil samples collected from Uttarkashi area of Garhwal Himalaya are presented. The correlation between radium content and radon mass exhalation rate from soil has also been obtained. The radium was measured by gamma ray spectrometry, while the mass exhalation rate of radon has been determined by both active and passive methods. The radium activity in the soil of study area was found to vary from 45±7 to 285±29 Bq kg(-1) with an average of 99 Bq kg(-1) The radon mass exhalation rate was found to vary from 0.59 × 10(-5) to 2.2 × 10(-5) Bq kg(-1) h(-1) with an average of 1.4 × 10(-5) Bq kg(-1) h(-1) by passive technique and from 0.8 × 10(-5) to 3.2 × 10(-5) Bq kg(-1) h(-1) with an average of 1.5 × 10(-5) Bq kg(-1) h(-1) by active technique. The results suggest that the measured radium value is positively correlated with the radon mass exhalation rate measured with both the active and passive techniques.
PMID: 27056143 [PubMed - in process]
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STUDY OF RADON FLUX FROM SOIL IN BUDHAKEDAR REGION USING SRM.
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STUDY OF RADON FLUX FROM SOIL IN BUDHAKEDAR REGION USING SRM.
Radiat Prot Dosimetry. 2016 Oct;171(2):267-270
Authors: Bourai AA, Aswal S, Kandari T, Kumar S, Joshi V, Sahoo BK, Ramola RC
Abstract
In the present study, the radon flux rate of the soil is measured using portable radon monitor (scintillation radon monitor) in the Budhakedar region of District Tehri, India. The study area falls along a fault zone named Main Central Thrust, which is relatively rich in radium-bearing minerals. Radon flux rate from the soil is one of the most important factors for the evaluation of environmental radon levels. The earlier studies in the Budhakedar region shows a high level of radon (>4000 Bq m(-3)). Hence, it is important to measure the radon flux rate. The aim of the present study is to calculate the average estimate of the surface radon flux rate as well as the effective mass exhalation rate. A positive correlation of 0.54 was found between radon flux rate and radon mass exhalation rate.
PMID: 27056141 [PubMed - in process]
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SOIL 222Rn CONCENTRATION, CO2 AND CH4 FLUX MEASUREMENTS AROUND THE JWALAMUKHI AREA OF NORTH-WEST HIMALAYAS, INDIA.
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SOIL 222Rn CONCENTRATION, CO2 AND CH4 FLUX MEASUREMENTS AROUND THE JWALAMUKHI AREA OF NORTH-WEST HIMALAYAS, INDIA.
Radiat Prot Dosimetry. 2016 Oct;171(2):262-266
Authors: Kumar A, Walia V, Yang TF, Fu CC, Singh S, Bajwa BS, Arora V
Abstract
Soil (222)Rn concentration, CO2 and CH4 flux measurements were conducted around the Jwalamukhi area of North-West Himalayas, India. During this study, around 37 soil gas points and flux measurements were taken with the aim to assure the suitability of this method in the study of fault zones. For this purpose, RAD 7 (Durridge, USA) was used to monitor radon concentrations, whereas portable diffuse flux meter (West Systems, Italy) was used for the CO2 and CH4 flux measurements. The recorded radon concentration varies from 6.1 to 34.5 kBq m(-3) with an average value of 16.5 kBq m(-3) The anomalous value of radon concentrations was recorded between Jwalamukhi thrust and Barsar thrust. The recorded average of CO2 and CH4 flux were 11.8 and 2.7 g m(-2) day(-1), respectively. The good correlation between anomalous CO2 flux and radon concentrations has been observed along the fault zone in the study area, suggesting that radon migration is dependent on CO2.
PMID: 27056140 [PubMed - in process]
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MEASUREMENT OF RADON, THORON AND THEIR PROGENY CONCENTRATIONS IN THE DWELLINGS OF PAURI GARHWAL, UTTARAKHAND, INDIA.
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MEASUREMENT OF RADON, THORON AND THEIR PROGENY CONCENTRATIONS IN THE DWELLINGS OF PAURI GARHWAL, UTTARAKHAND, INDIA.
Radiat Prot Dosimetry. 2016 Oct;171(2):234-237
Authors: Joshi V, Dutt S, Yadav M, Mishra R, Ramola RC
Abstract
It is well known that inhalation of radon, thoron and their progeny contributes more than 50 % of natural background radiation dose to human being. The time-integrated passive measurements of radon, thoron and their progeny concentrations were carried out in the dwellings of Pauri Garhwal, Uttarakhand, India. The measurements of radon and thoron concentrations were performed by LR-115 detector-based single-entry pin-hole dosemeter, while for the measurement of progeny concentrations, LR-115 deposition-based direct radon and thoron progeny sensors technique was used. The experimental techniques and results obtained are discussed in detail.
PMID: 27056139 [PubMed - in process]
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A faecal exposure assessment of farm workers in Accra, Ghana: a cross sectional study
Antwi-Agyei, P; Biran, A; Peasey, A; Bruce, J; Ensink, J; (2016) A faecal exposure assessment of farm workers in Accra, Ghana: a cross sectional study. BMC PUBLIC HEALTH , 16 (ARTN 587) 10.1186/s12889-016-3266-8 .
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The role of awareness in spatial integration
Schwarzkopf, DS; Banica, T; (2015) The role of awareness in spatial integration. PERCEPTION , 44 (4) p. 466.
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H2 fuel synthesis from water by a highly efficient and robust system
Tang, J; jiang, C; wu, J; Liu, H; H2 fuel synthesis from water by a highly efficient and robust system.
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Analysing radiative and non-radiative recombination in InAs QDs on Si for integrated laser applications
Orchard, JR; Woodhead, C; Shutts, S; Wu, J; Sobiesierski, A; Young, RJ; Beanland, R; Orchard, JR; Woodhead, C; Shutts, S; Wu, J; Sobiesierski, A; Young, RJ; Beanland, R; Liu, H; Smowton, PM; Mowbray, DJ; - view fewer (2016) Analysing radiative and non-radiative recombination in InAs QDs on Si for integrated laser applications. In: Huffaker, DL and Eisele, H and Dick, KA, (eds.) (Proceedings) Conference on Quantum Dots and Nanostructures - Growth, Characterization, and Modeling XIII. SPIE-INT SOC OPTICAL ENGINEERING
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Growth of High-Quality Self-Catalyzed Core-Shell GaAsP Nanowires on Si Substrates
Zhang, Y; Aagesen, M; Sanchez, AM; Wu, J; Beanland, R; Ward, T; Kim, D; Zhang, Y; Aagesen, M; Sanchez, AM; Wu, J; Beanland, R; Ward, T; Kim, D; Jurczak, P; Huo, S; Liu, H; - view fewer (2016) Growth of High-Quality Self-Catalyzed Core-Shell GaAsP Nanowires on Si Substrates. In: Huffaker, DL and Eisele, H and Dick, KA, (eds.) (Proceedings) Conference on Quantum Dots and Nanostructures - Growth, Characterization, and Modeling XIII. SPIE-INT SOC OPTICAL ENGINEERING
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Autosomal dominant polycystic kidney disease
Burns, A; (1995) Autosomal dominant polycystic kidney disease. In: Pounder, R and Hamilton, M, (eds.) Handbook of current diagnoses and treatment. Churchill Livingstone
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Chronic renal failure
Burns, A; (1995) Chronic renal failure. In: Pounder, R and Hamilton, M, (eds.) UNSPECIFIED
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Connective tissue disease and acute renal failure
Burns, A; (2002) Connective tissue disease and acute renal failure. In: Allen, A and Glynne, P and Pusey, C, (eds.) Acute renal failure in practice. Imperial College Press
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Development of the enteric neuromuscular system
Heanue, TA; Burns, AJ; (2017) Development of the enteric neuromuscular system. In: Faure, C and Thapar, N and Di Lorenzo, C, (eds.) Pediatric Neurogastroenterology: Gastrointestinal Motility and Functional Disorders in Children. Springer International Publishing
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Developing pupils’ self-assessment in the primary classroom
Dann, R; (2012) Developing pupils' self-assessment in the primary classroom. In: Cable,, C and Eyres, I, (eds.) Primary Teaching Assistants: Curriculum in Context. (pp. 171-176). Open University press
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Transition To Mentoring: Issues in Primary Initial Teacher Education
Dann, R; (1995) Transition To Mentoring: Issues in Primary Initial Teacher Education. In: Researching School-Based Teacher Education. (pp. 59-71). Avebury: Aldershot.
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Preparation for Year 6 National Tests: Examining the Interrelationships of Teaching, Learning and Assessment
Dann, R; (1999) Preparation for Year 6 National Tests: Examining the Interrelationships of Teaching, Learning and Assessment. Education 3-13 27(2)
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Can We Improve the Reproducibility of Quantitative Multiparametric Prostate MR Imaging Metrics?
Johnston, E; Punwani, S; (2016) Can We Improve the Reproducibility of Quantitative Multiparametric Prostate MR Imaging Metrics? Radiology , 281 (2) pp. 652-653. 10.1148/radiol.2016161197 .
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) Teacher-Mentor to Teacher-Researcher
Dann, R; (1996) ) Teacher-Mentor to Teacher-Researcher. Mentoring and Tutoring Vol. 3 No. 3, March,
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Developing Pupils’ Skills in Self-Assessment
Dann, R; (1996) Developing Pupils' Skills in Self-Assessment. Primary Practice 1996 June (5)
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Development of Keap1-interactive small molecules that regulate Nrf2 transcriptional activity
Georgakopoulos, ND; Gatliff, J; Wells, G; (2016) Development of Keap1-interactive small molecules that regulate Nrf2 transcriptional activity. Current Opinion in Toxicology , 1 (C) pp. 1-8. 10.1016/j.cotox.2016.08.002 . (In press).
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A novel presenilin 1 L166H mutation in a pseudo-sporadic case of early-onset Alzheimer's disease.
Pantieri, R; Pardini, M; Cecconi, M; Dagna-Bricarelli, F; Vitali, A; Piccini, A; Russo, R; Pantieri, R; Pardini, M; Cecconi, M; Dagna-Bricarelli, F; Vitali, A; Piccini, A; Russo, R; Borghi, R; Tabaton, M; - view fewer (2005) A novel presenilin 1 L166H mutation in a pseudo-sporadic case of early-onset Alzheimer's disease. Neurol Sci , 26 (5) pp. 349-350. 10.1007/s10072-005-0499-1 .
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Integral calculus problem solving: an fMRI investigation.
Krueger, F; Spampinato, MV; Pardini, M; Pajevic, S; Wood, JN; Weiss, GH; Landgraf, S; Krueger, F; Spampinato, MV; Pardini, M; Pajevic, S; Wood, JN; Weiss, GH; Landgraf, S; Grafman, J; - view fewer (2008) Integral calculus problem solving: an fMRI investigation. Neuroreport , 19 (11) pp. 1095-1099. 10.1097/WNR.0b013e328303fd85 .
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Age-related decline in mentalizing skills across adult life span.
Pardini, M; Nichelli, PF; (2009) Age-related decline in mentalizing skills across adult life span. Exp Aging Res , 35 (1) pp. 98-106. 10.1080/03610730802545259 .
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Autologous haematopoietic stem-cell transplantation in multiple sclerosis: benefits and risks.
Capello, E; Vuolo, L; Gualandi, F; Van Lint, MT; Roccatagliata, L; Bonzano, L; Pardini, M; Capello, E; Vuolo, L; Gualandi, F; Van Lint, MT; Roccatagliata, L; Bonzano, L; Pardini, M; Uccelli, A; Mancardi, G; - view fewer (2009) Autologous haematopoietic stem-cell transplantation in multiple sclerosis: benefits and risks. Neurol Sci , 30 Sup S175-S177. 10.1007/s10072-009-0144-5 .
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The role of awareness in spatial integration
Schwarzkopf, DS; Banica, T; (2015) The role of awareness in spatial integration. PERCEPTION , 44 (4) p. 466.
http://ift.tt/2e1kVxF
H2 fuel synthesis from water by a highly efficient and robust system
Tang, J; jiang, C; wu, J; Liu, H; H2 fuel synthesis from water by a highly efficient and robust system.
http://ift.tt/2ftsUbX
Analysing radiative and non-radiative recombination in InAs QDs on Si for integrated laser applications
Orchard, JR; Woodhead, C; Shutts, S; Wu, J; Sobiesierski, A; Young, RJ; Beanland, R; Orchard, JR; Woodhead, C; Shutts, S; Wu, J; Sobiesierski, A; Young, RJ; Beanland, R; Liu, H; Smowton, PM; Mowbray, DJ; - view fewer (2016) Analysing radiative and non-radiative recombination in InAs QDs on Si for integrated laser applications. In: Huffaker, DL and Eisele, H and Dick, KA, (eds.) (Proceedings) Conference on Quantum Dots and Nanostructures - Growth, Characterization, and Modeling XIII. SPIE-INT SOC OPTICAL ENGINEERING
http://ift.tt/2e1mGLe
Growth of High-Quality Self-Catalyzed Core-Shell GaAsP Nanowires on Si Substrates
Zhang, Y; Aagesen, M; Sanchez, AM; Wu, J; Beanland, R; Ward, T; Kim, D; Zhang, Y; Aagesen, M; Sanchez, AM; Wu, J; Beanland, R; Ward, T; Kim, D; Jurczak, P; Huo, S; Liu, H; - view fewer (2016) Growth of High-Quality Self-Catalyzed Core-Shell GaAsP Nanowires on Si Substrates. In: Huffaker, DL and Eisele, H and Dick, KA, (eds.) (Proceedings) Conference on Quantum Dots and Nanostructures - Growth, Characterization, and Modeling XIII. SPIE-INT SOC OPTICAL ENGINEERING
http://ift.tt/2ftsiDn
Autosomal dominant polycystic kidney disease
Burns, A; (1995) Autosomal dominant polycystic kidney disease. In: Pounder, R and Hamilton, M, (eds.) Handbook of current diagnoses and treatment. Churchill Livingstone
http://ift.tt/2e1oYdd
Chronic renal failure
Burns, A; (1995) Chronic renal failure. In: Pounder, R and Hamilton, M, (eds.) UNSPECIFIED
http://ift.tt/2fts9zN
Connective tissue disease and acute renal failure
Burns, A; (2002) Connective tissue disease and acute renal failure. In: Allen, A and Glynne, P and Pusey, C, (eds.) Acute renal failure in practice. Imperial College Press
http://ift.tt/2e1pg3Z
Development of the enteric neuromuscular system
Heanue, TA; Burns, AJ; (2017) Development of the enteric neuromuscular system. In: Faure, C and Thapar, N and Di Lorenzo, C, (eds.) Pediatric Neurogastroenterology: Gastrointestinal Motility and Functional Disorders in Children. Springer International Publishing
http://ift.tt/2ftqtWP
Developing pupils’ self-assessment in the primary classroom
Dann, R; (2012) Developing pupils' self-assessment in the primary classroom. In: Cable,, C and Eyres, I, (eds.) Primary Teaching Assistants: Curriculum in Context. (pp. 171-176). Open University press
http://ift.tt/2e1q3C0
Transition To Mentoring: Issues in Primary Initial Teacher Education
Dann, R; (1995) Transition To Mentoring: Issues in Primary Initial Teacher Education. In: Researching School-Based Teacher Education. (pp. 59-71). Avebury: Aldershot.
http://ift.tt/2ftt1EG
Preparation for Year 6 National Tests: Examining the Interrelationships of Teaching, Learning and Assessment
Dann, R; (1999) Preparation for Year 6 National Tests: Examining the Interrelationships of Teaching, Learning and Assessment. Education 3-13 27(2)
http://ift.tt/2e1pfgr
Can We Improve the Reproducibility of Quantitative Multiparametric Prostate MR Imaging Metrics?
Johnston, E; Punwani, S; (2016) Can We Improve the Reproducibility of Quantitative Multiparametric Prostate MR Imaging Metrics? Radiology , 281 (2) pp. 652-653. 10.1148/radiol.2016161197 .
http://ift.tt/2ftnAp4
) Teacher-Mentor to Teacher-Researcher
Dann, R; (1996) ) Teacher-Mentor to Teacher-Researcher. Mentoring and Tutoring Vol. 3 No. 3, March,
http://ift.tt/2e1kP9p
Developing Pupils’ Skills in Self-Assessment
Dann, R; (1996) Developing Pupils' Skills in Self-Assessment. Primary Practice 1996 June (5)
http://ift.tt/2ftqsSL
Development of Keap1-interactive small molecules that regulate Nrf2 transcriptional activity
Georgakopoulos, ND; Gatliff, J; Wells, G; (2016) Development of Keap1-interactive small molecules that regulate Nrf2 transcriptional activity. Current Opinion in Toxicology , 1 (C) pp. 1-8. 10.1016/j.cotox.2016.08.002 . (In press).
http://ift.tt/2e1qr3h
A faecal exposure assessment of farm workers in Accra, Ghana: a cross sectional study
Antwi-Agyei, P; Biran, A; Peasey, A; Bruce, J; Ensink, J; (2016) A faecal exposure assessment of farm workers in Accra, Ghana: a cross sectional study. BMC PUBLIC HEALTH , 16 (ARTN 587) 10.1186/s12889-016-3266-8 .
http://ift.tt/2ftpAOf
A novel presenilin 1 L166H mutation in a pseudo-sporadic case of early-onset Alzheimer's disease.
Pantieri, R; Pardini, M; Cecconi, M; Dagna-Bricarelli, F; Vitali, A; Piccini, A; Russo, R; Pantieri, R; Pardini, M; Cecconi, M; Dagna-Bricarelli, F; Vitali, A; Piccini, A; Russo, R; Borghi, R; Tabaton, M; - view fewer (2005) A novel presenilin 1 L166H mutation in a pseudo-sporadic case of early-onset Alzheimer's disease. Neurol Sci , 26 (5) pp. 349-350. 10.1007/s10072-005-0499-1 .
http://ift.tt/2e1ltUo
Integral calculus problem solving: an fMRI investigation.
Krueger, F; Spampinato, MV; Pardini, M; Pajevic, S; Wood, JN; Weiss, GH; Landgraf, S; Krueger, F; Spampinato, MV; Pardini, M; Pajevic, S; Wood, JN; Weiss, GH; Landgraf, S; Grafman, J; - view fewer (2008) Integral calculus problem solving: an fMRI investigation. Neuroreport , 19 (11) pp. 1095-1099. 10.1097/WNR.0b013e328303fd85 .
http://ift.tt/2fts7b9
Age-related decline in mentalizing skills across adult life span.
Pardini, M; Nichelli, PF; (2009) Age-related decline in mentalizing skills across adult life span. Exp Aging Res , 35 (1) pp. 98-106. 10.1080/03610730802545259 .
http://ift.tt/2e1nLmk
Autologous haematopoietic stem-cell transplantation in multiple sclerosis: benefits and risks.
Capello, E; Vuolo, L; Gualandi, F; Van Lint, MT; Roccatagliata, L; Bonzano, L; Pardini, M; Capello, E; Vuolo, L; Gualandi, F; Van Lint, MT; Roccatagliata, L; Bonzano, L; Pardini, M; Uccelli, A; Mancardi, G; - view fewer (2009) Autologous haematopoietic stem-cell transplantation in multiple sclerosis: benefits and risks. Neurol Sci , 30 Sup S175-S177. 10.1007/s10072-009-0144-5 .
http://ift.tt/2ftnQV0
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