Bounce regime of droplet collisions: A molecular dynamics study

Zhang, YR; Jiang, XZ; Luo, KH; (2016) Bounce regime of droplet collisions: A molecular dynamics study. Journal of Computational Science 10.1016/j.jocs.2016.03.011 . (In press). Green open access

from #MedicinebyAlexandrosSfakianakis via xlomafota13 on Inoreader http://ift.tt/28Y1qoN
via IFTTT

Accurate sample assignment in a multiplexed, ultrasensitive, high-throughput sequencing assay for minimal residual disease

Bartram, J; Mountjoy, E; Brooks, T; Hancock, J; Williamson, H; Wright, G; Moppett, J; Bartram, J; Mountjoy, E; Brooks, T; Hancock, J; Williamson, H; Wright, G; Moppett, J; Goulden, N; Hubank, M; - view fewer (2016) Accurate sample assignment in a multiplexed, ultrasensitive, high-throughput sequencing assay for minimal residual disease. Journal of Molecular Diagnostics , 18 (4) pp. 494-506. 10.1016/j.jmoldx.2016.02.008 . Green open access

from #MedicinebyAlexandrosSfakianakis via xlomafota13 on Inoreader http://ift.tt/28S6zD5
via IFTTT

The psychology of suggestion and heightened suggestibility.

Gandhi, B.; (2006) The psychology of suggestion and heightened suggestibility. Doctoral thesis, University of London. Green open access

from #MedicinebyAlexandrosSfakianakis via xlomafota13 on Inoreader http://ift.tt/28Y1qFg
via IFTTT

The preparation and characterisation of cells and their scaffolds suitable for tissue repair applications.

Pittarello, L.; (2008) The preparation and characterisation of cells and their scaffolds suitable for tissue repair applications. Doctoral thesis, University of London. Green open access

from #MedicinebyAlexandrosSfakianakis via xlomafota13 on Inoreader http://ift.tt/28S6BuH
via IFTTT

Different HIV-1 env frames: gp120 and ASP (antisense protein) biosynthesis, and theirs co-variation tropic amino acid signatures in X4- and R5-viruses

Abstract

Antisense protein (ASP) is the new actor of viral life of Human Immunodeficiency Virus type 1 (HIV-1) although proposed above 20 years ago. The asp ORF is into complementary strand of the gp120/gp41 junction of env gene. The ASP biological role remains little known. Knowing the Env markers of viral tropism, a dataset of sequences (660 strains) was used to analyze the hypothetical ASP involvement in CCR5 (R5) and/or CXCR4 (X4) co-receptor interaction. Preliminarily, prevalence of ASP and gp120_V3 mutations was performed; following association among mutations were elaborate. The classical V3 tropic-signatures were confirmed, and 36 R5- and 22 × 4-tropic ASP mutations were found. Moreover, by analyzing the ASP sequences, 36 out of 179 amino acid positions significantly associated with different co-receptor usage were found. Several statistically significant associations between gp120_V3 and ASP mutations were observed. The dendrogram showed the existence of a cluster associated with R5-usage and a large cluster associated with X4-usage. These results show that gp120_V3 and specific amino acid changes in ASP are associated together with CXCR4 and/or CCR5-usage. These findings implement previous observations on unclear ASP functions. This article is protected by copyright. All rights reserved

from #MedicinebyAlexandrosSfakianakis via xlomafota13 on Inoreader http://ift.tt/28Nhf2x
via IFTTT

Epidemiology and genetic diversity of group A rotavirus in acute diarrhea patients in pre-vaccination era in southwest China

Abstract

Group A rotavirus (RVA) is one of the leading cause of acute diarrhea worldwide, the RVA-related disease burden and the genotypes of RVA is important reference to introduce RVA variance to national immunisation programmes, 1,121 diarrhea cases and 319 healthy controls were recruited from four sentinel hospital outpatient from July 2014 to June 2015. The prevalence of RVA was 244 (21.8%) in gastroenteritis cases and in 12 (3.8%) in healthy controls across all age group (OR = 7.12, 95%CI = 3.93–12.89); the detection rate of RVA in diarrhea patients under five years was more higher than in diarrhea cases over five years (26.1%, 222/850; 8.1%, 22/271, respectively, P = 0.000), Of 244 RVA strains isolated from acute diarrhea cases, G9 (66.4%) was predominant G genotype, followed by G3 (18.7%), G1 (8.9%) and G1G3 (3.8%); P[8] was the overwhelming prevalence P genotype, followed by P[4] (4.7%); G9P[8] (54.9%) was most common G and P Combination, followed by G3P[8] (17.6%) and G1[8] (8.6%). The conclusion of the study was important to provide reference for introducing the RVA vaccine to prevent and control RVA-associated disease burden. This article is protected by copyright. All rights reserved

from #MedicinebyAlexandrosSfakianakis via xlomafota13 on Inoreader http://ift.tt/28Ogw4J
via IFTTT

Impact of resistance-associated variant dominancy on treatment in patients with HCV genotype 1b receiving daclatasvir/asunaprevir

Abstract

Sustained virological responses (SVR) by daclatasvir (DCV) and asunaprevir (ASV) therapy for genotype 1b hepatitis C virus (HCV) infected patients has been significantly affected by pre-existence of Y93H resistance-associated variants (RAVs) in the non-structural protein 5A (NS5A) region. The aim of this study was to elucidate the dominancy of naturally occurring RAVs in viral quasispecies on treatment outcomes in patients with HCV. In total, 138 patients were prospectively selected from 152 patients treated with DCV and ASV, where evaluation of treatment outcomes at 12 weeks post-treatment was possible. Pre-treatment RAVs in the non-structural protein 3 and NS5A regions were detected by polymerase chain reaction (PCR)-Invader assays, and the ratio of Y93H RAVs in viral quasispecies was measured by quantitative PCR-Invader assay. Among 25 patients detected the Y93H RAV, the Y93H ratio was 1–25% in 5 patients, 26–75% in 7 patients, and ≥76% in 13 patients. Overall, SVR at 12 weeks after the completion of treatment (SVR12) was 91% (125/138), and those with Y93H ratios of <1%, 1–25%, 26–75%, and ≥76% were 99%, 100%, 71%, and 23%, respectively. Thus, the SVR12 decreased as the HCV Y93H ratio increased (p < 0.0001). The dominancy of pre-treatment RAVs of DCV and ASV affected its treatment outcomes, suggesting that evaluating the dominancy of HCV RAVs could be required for every other direct-acting antiviral agent treatments. This article is protected by copyright. All rights reserved

from #MedicinebyAlexandrosSfakianakis via xlomafota13 on Inoreader http://ift.tt/28Nhgn0
via IFTTT