Retroviral Scanning: Mapping MLV Integration Sites to Define Cell-specific Regulatory Regions

Here, we describe a protocol for genome-wide mapping of the integration sites of Moloney murine leukemia virus-based retroviral vectors in human cells.

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Physical Mechanical characterization of cosmetic formulations and correlation between instrumental measurements and sensorial properties

Abstract

Objective

The correct choice of raw materials in the development of cosmetic formulations is essential for obtaining stable and pleasant skin care products. Therefore, rheological, texture and sensory analyses are important to understand the behavior and stability of the formulations. In this context, the aim of the present study was to develop cosmetic formulations containing or not (vehicle) UV filters and chicory root extract, to evaluate their stability as well as to characterize their physical and texture properties and correlate them with the sensory attributes.

Methods

Four formulations containing organic UV filters and chicory extract, each alone or in combination, were developed and evaluated for 180 days with a cone and plate rheometer, a texture analyzer and consumer's sensorial analysis. Thus, the data obtained were correlated in order to observe the different influences.

Results

The developed formulations remained stable after 180 days regarding macroscopic aspects, organoleptic characteristics and pH values. The addition of the UV filters alone and in combination with the active substance resulted in significant increases in rheology, viscosity and consistency. The formulation with the active ingredient showed significant decreases in the texture parameters after 180 days, mainly due to its polysaccharide Inulin. All formulations obtained high scores in sensorial parameters. A strong correlation was mainly found between spreadability and work of shear, and between the texture parameters.

Conclusion

The raw materials strongly influenced the physical, texture and sensorial parameters. Finally, the UV filters showed a greater influence on the results of the formulations than the chicory root extract. In conclusion, the association of the mentioned methods allows the correct choice of ingredients and their combinations.

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Assessment of Spontaneous Alternation, Novel Object Recognition and Limb Clasping in Transgenic Mouse Models of Amyloid-β and Tau Neuropathology

Described here is a staged, behavioral screening approach that can be used to screen for compounds that exhibit in vivo efficacy on cognitive and functional motor behaviors in transgenic mouse models of β-amyloidosis and tauopathy. These methods are optimized to screen compounds for activity in short-term and working memory tasks.

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Oral lichenoid dysplasia and exophytic oral verrucous hyperplasia: Mythical entities of oral pathology

Publication date: Available online 27 May 2017
Source:Oral Oncology
Author(s): A. Thirumal Raj




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Editorial Board/Aims & Scope

Publication date: June 2017
Source:Oral Oncology, Volume 69





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In Memoriam – Crispian Scully 1945–2017

Publication date: June 2017
Source:Oral Oncology, Volume 69
Author(s): Jatin P. Shah




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Oral lichenoid dysplasia and exophytic oral verrucous hyperplasia: Mythical entities of oral pathology

Publication date: Available online 27 May 2017
Source:Oral Oncology
Author(s): A. Thirumal Raj




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