Down-regulation of liver-intestine cadherin enhances noscapine-induced apoptosis in human colon cancer cells.

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Down-regulation of liver-intestine cadherin enhances noscapine-induced apoptosis in human colon cancer cells.

Expert Rev Anticancer Ther. 2017 Jun 16;:

Authors: Tian X, Liu M, Zhu Q, Tan J, Liu W, Wang Y, Chen W, Zou Y, Cai Y, Han Z, Huang X

Abstract
OBJECTIVES: Liver-intestine cadherin (CDH17) as a novel type of cadherin can significantly suppresses tumor growth in colon cancer cells. Noscapine is one of the most widely used antineoplastic agents in the treatment of solid tumor and haematological malignancies. The aim of the present study was to explore the signaling pathway of noscapine which induces apoptosis by blocking CDH17 gene in colon cancer SW480 cells.
METHODS: Human colon cancer SW480 cells were transfected with CDH17 interference vector and treatment with 10 µmol/L noscapine. The proliferation of SW480 cells were detected by MTT assay and the apoptosis of SW480 cells were detected by AnnexinV-FITC/PI flow cytometry kit (BD). Cell invasion were assessed by transwell assays. Apoptosis related proteins (Cyt-c, Bax, Bcl-2 and Bcl-xL) levels were evaluated by western blot.
RESULTS: Our results showed that compared to the noscapine group, proliferation was decreased significantly and the apoptosis was increased significantly in SW480 cells of the group of siCDH17+noscapine. The protein levels of Cyt-c and Bax in siCDH17+noscapine group was higher than that of the noscapine group, but the protein levels of Bcl-2 and Bcl-xL in siCDH17+noscapine group were lower than that of the noscapine group, which suggests that the apoptosis is induced by noscapine and siCDH17 promotes the efficiency of noscapine-induced apoptosis. Moreover, up-expression of CDH17 inhibited the efficacy of noscapine-induced apoptosis in SW480 cells.
CONCLUSIONS: We inferred that down-expression of extrinsic CDH17 gene can conspicuously promote apoptosis-inducing effects of noscapine on human colon cancer SW480 cells, which is a novel strategy to improve chemotherapeutic effects on colon cancer.

PMID: 28622054 [PubMed - as supplied by publisher]

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