Αρχειοθήκη ιστολογίου

Τετάρτη 23 Ιανουαρίου 2019

Dysregulated fatty acid metabolism in nasal polyp‐derived eosinophils from patients with chronic rhinosinusitis

Abstract

Background

Eosinophils are multifunctional granulocytes capable of releasing various cytokines, chemokines, and lipid mediators. We previously reported dysregulated fatty acid metabolism in peripheral blood‐derived eosinophils from patients with severe asthma. However, functional characteristics of eosinophils present in allergic inflammatory tissues remains largely uncharacterized.

Methods

We established a method for isolating CD69hi CCR3low CXCR4 siglec‐8int eosinophils from nasal polyps of patients with eosinophilic rhinosinusitis (NP‐EOS). Multi‐omics analysis including lipidomics, proteomics, and transcriptomics was performed to analyze NP‐EOS as compared with peripheral blood‐derived eosinophils from healthy subjects (PB‐EOS).

Results

Lipidomic analysis revealed impaired synthesis of prostaglandins and 15‐lipoxygenase (15‐LOX)‐derived mediators, and selective upregulation of leukotriene D4 production. Furthermore, proteomics and transcriptomics revealed changes in the expression of specific enzymes (GGT5, DPEP2, and 15‐LOX) responsible for dysregulated lipid metabolism. Ingenuity pathway analysis indicated the importance of type 2 cytokines and pattern recognition receptor pathways. Stimulation of PB‐EOS with eosinophil activators IL‐5, GM‐CSF, and agonists of TLR2 and NOD2 mimicked the observed changes in lipid metabolism.

Conclusion

Inflammatory tissue‐derived eosinophils possess a specific phenotype with dysregulated fatty acid metabolism that may be targeted therapeutically to control eosinophilic inflammatory diseases.

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