Background: Genome-wide studies in higher eukaryotes have revealed the presence of paused RNA polymerase II (RNA-Pol) at about 30–50 bp downstream of the transcription start site of genes involved in developmental control, cell proliferation and intercellular signaling. Promoter-proximal pausing is believed to represent a critical step in transcriptional regulation. GAGA sequence motifs have frequently been found in the upstream region of paused genes in Drosophila, implicating a prevalent binding factor, GAF, in transcriptional pausing. Results: Using newly isolated mutants that retain only ~3 % normal GAF level, we analyzed its impacts on transcriptional regulation in whole animals. We first examined the abundance of three major isoforms of RNA-Pol on Hsp70 during heat shock. By cytogenetic analyses on polytene chromosomes and chromatin immunoprecipitation (ChIP), we show that paused RNA-Pol of Hsp70 is substantially reduced in mutants. Conversely, a global increase in paused RNA-Pol is observed when GAF is over-expressed. Coupled analyses of transcriptome and GAF genomic distribution show that 269 genes enriched for upstream GAF binding are down-regulated in mutants. Interestingly, ~15 % of them encode transcriptional factors, which might control ~2000 additional genes down-regulated in mutants. Further examination of RNA-Pol distribution in GAF targets reveals that a positive correlation exists between promoter-proximal RNA-Pol density and GAF occupancy in WT, but not in mutants. Comparison of nucleosome profiles indicates that nucleosome occupancy is preferentially attenuated by GAF in the upstream region that strongly favors nucleosome assembly. Using a dominant eye phenotype caused by GAF over-expression, we detect significant genetic interactions between GAF and the nucleosome remodeler NURF, the pausing factor NELF, and BAB1 whose binding sites are enriched specifically in genes displaying GAF-dependent pausing. Conclusion: Our results provide direct evidence to support a critical role of GAF in global gene expression, transcriptional pausing and upstream nucleosome organization of a group of genes. By cooperating with factors acting at different levels, GAF orchestrates a series of events from local nucleosome displacement to paused transcription. The use of whole animals containing broad tissue types attests the physiological relevance of this regulatory network.
from #MedicinebyAlexandrosSfakianakis via xlomafota13 on Inoreader http://ift.tt/2awwEI0
via IFTTT
Αρχειοθήκη ιστολογίου
-
►
2020
(289)
- ► Φεβρουαρίου (28)
-
►
2019
(9071)
- ► Δεκεμβρίου (19)
- ► Σεπτεμβρίου (54)
- ► Φεβρουαρίου (3642)
- ► Ιανουαρίου (3200)
-
►
2018
(39872)
- ► Δεκεμβρίου (3318)
- ► Σεπτεμβρίου (3683)
- ► Φεβρουαρίου (2693)
- ► Ιανουαρίου (3198)
-
►
2017
(41099)
- ► Δεκεμβρίου (3127)
- ► Σεπτεμβρίου (2173)
-
▼
2016
(13807)
- ► Δεκεμβρίου (700)
- ► Σεπτεμβρίου (600)
-
▼
Ιουλίου
(1200)
-
▼
Ιουλ 27
(50)
- Aluminium Salalens vs. Salans: "Initiator Design" ...
- Hydrogen cleavage by solid-phase frustrated Lewis ...
- The Thoracic Peri-Operative Integrative Surgical C...
- Regorafenib in Combination With Paclitaxel in Adva...
- Feasibility Study of Adjuvant Treatment With S-1 a...
- The Addition of Nab-paclitaxel (Abraxane) to First...
- Feasibility Study of Chemoradiation, TRAstuzumab a...
- Patient satisfaction in the management of mild-to-...
- Collagenous colitis and Crohn's disease: Guilty or...
- HCV novel therapeutic regimens in Wonderland: A bu...
- Effectiveness of Endoscopic Ultrasonography during...
- An increase in serum tumour necrosis factor-α duri...
- Früherkennung
- RANZCR 2016 Case of the Day competition
- Corrigendum to “Magnesium alloys as body implants:...
- Brain stiffness increases with myelin content
- Study of Antiobesity Effect through Inhibition of ...
- Traumatic Renal Artery Thrombosis in a Child A cas...
- Incidental cervical metastases from thyroid carcin...
- Deviations of different systems for guided implant...
- Quality control of house dust mite extracts by bro...
- Toxins, Vol. 8, Pages 231: Susceptibility of Broil...
- Comparative evaluation of different doses of cipro...
- Innovative new method to test skill of bioassay in...
- Use of over the counter drugs in urban and rural p...
- Bacterial infections in cirrhosis - does standard ...
- Assessment of medication adherence among hypertens...
- A comparative study of adverse reactions of taxol ...
- To study the pattern of suspected adverse drug rea...
- Drug utilization study in patients with type 2 dia...
- Comparative study to evaluate the anti-diabetic ac...
- Cost analysis of antiepileptic drugs available in ...
- Fluconazole induced multifocal bullous eruptions: ...
- Hand tremor possibly induced by interaction of ris...
- Phenylephrine induced fixed drug eruption: a rare ...
- A rare case of imatinib mesylate induced acute kid...
- Pharmacoeconomic analysis of drugs used for peptic...
- Studies on the antitumor potentials of betulinic a...
- Assessment of inhalation technique and predictors ...
- Study of prescription pattern and adverse drug rea...
- Assessment of prescription writing skills among un...
- Drug utilization study of anti-hypertensive drugs ...
- Evaluation of Voice Acoustics as Predictors of Cli...
- Towards Objective Voice Assessment: The Diplophoni...
- Erratum to: Clinical profile of tetanus patients a...
- The relation of weight suppression and BMIz to bul...
- Fertility preservation in women with cervical, end...
- GAGA factor, a positive regulator of global gene e...
- Cancers, Vol. 8, Pages 71: CD8 T Cell–Independent ...
- Advantages, limitations, and diagnostic accuracy o...
-
▼
Ιουλ 27
(50)
- ► Φεβρουαρίου (1350)
- ► Ιανουαρίου (1400)
-
►
2015
(1500)
- ► Δεκεμβρίου (1450)
Ετικέτες
Τετάρτη 27 Ιουλίου 2016
GAGA factor, a positive regulator of global gene expression, modulates transcriptional pausing and organization of upstream nucleosomes
Εγγραφή σε:
Σχόλια ανάρτησης (Atom)
Δεν υπάρχουν σχόλια:
Δημοσίευση σχολίου