Αρχειοθήκη ιστολογίου

Παρασκευή 14 Ιουλίου 2017

A comprehensive study of smoking-specific microRNA alterations in head and neck squamous cell carcinoma

Publication date: September 2017
Source:Oral Oncology, Volume 72
Author(s): Aswini R. Krishnan, Hao Zheng, James G. Kwok, Yuanhao Qu, Angela E. Zou, Avinaash Korrapati, Pin Xue Li, Joseph A. Califano, Melbourne F. Hovell, Jessica Wang-Rodriguez, Weg M. Ongkeko
ObjectiveWhile tobacco smoking is a well-known risk factor for head and neck squamous cell carcinoma (HNSCC), the molecular mechanisms underlying tobacco-induced HNSCC remain unclear. This study sought to comprehensively identify microRNA (miRNA) alterations and evaluate their clinical relevance in smoking-induced HNSCC pathogenesis and progression.Materials and methodsUsing small RNA-sequencing data and clinical data from 145 HNSCC patients, we performed a series of differential expression and correlation analyses to identify a panel of tobacco-dysregulated miRNAs associated with key clinical characteristics in HNSCC. We then examined the expression patterns of these miRNAs in normal epithelial cell lines following exposure to cigarette smoke extract.ResultsOur analyses revealed distinct panels of miRNAs to be dysregulated with smoking status and associated with additional clinical features, including tumor stage, metastasis, anatomic site, and patient survival. The differential expression of key miRNAs, including miR-101, miR-181b, miR-486, and miR-1301, was verified in cigarette-treated epithelial cell lines, suggesting their potential roles in the early development of smoking-related HNSCCs.ConclusionSpecific alterations in miRNA expression may be traced to tobacco use and are associated with important HNSCC clinical characteristics. Future studies of these miRNAs may be valuable for furthering the understanding and targeted treatment of smoking-associated HNSCC.



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