Publication date: December 2017
Source:Journal of Dairy Science, Volume 100, Issue 12
Author(s): J.G. Laguna, M.S. Cardoso, J.A. Lima, R.B. Reis, A.U. Carvalho, H.M. Saturnino, S.M.R. Teixeira
The aim of this study was to investigate the expression of genes encoding enzymes and other factors involved with carbohydrate and lipid metabolism in the liver of 2 genetic groups of dairy cows during the transition period. We analyzed the expression of glucose-6-phosphatase (G6PC), cytosolic phosphoenolpyruvate carboxykinase (PEPCK-C), methylmalonyl-CoA mutase (MUT), β-hydroxybutyrate dehydrogenase-2 (BDH2), acetyl-CoA carboxylase (ACC), carnitine palmitoyltransferase-2 (CPT2), 3-hydroxy-3-methylglutaryl-CoA reductase (HMGCR), glucose transporter-2 (SLC2A2), and the transcription factor peroxisome proliferator-activated receptor α (PPARA). Blood concentrations of glucose, nonesterified fatty acids, and β-hydroxybutyrate were also determined. Liver biopsies and blood samples were taken at d 15 prepartum and at d 6, 21, 36, 51, and 66 postpartum from Holsteins (n = 6) and F1 Holstein-Gir (n = 6) cows. Cows were kept under the same prepartum and postpartum management conditions. The results showed that the expression of G6PC, PEPCK-C, BDH2, ACC, CPT2, HMGCR, SLC2A2, and PPARA genes did not differ between genetic groups. Except for PEPCK-C, no interaction between genetic groups and the experimental period was observed. Within both groups of cows, G6PC and PEPCK-C gene expression decreased when comparing prepartum gene expression with 21 and 36 DIM, and increased in d 51 postpartum. MUT mRNA levels differed between the 2 genetic groups and displayed a significant increase after d 36 postpartum, whereas mRNA levels of HMGCR tended to increase when comparing d 21 and 36 to d 51 postpartum. Glucose concentrations also differed between genetic groups, being significantly higher in the plasma of F1 Holstein-Gir cows than in Holstein cows, but no differences were found within each group during the analysis period. β-Hydroxybutyrate and nonesterified fatty acid concentrations did not differ between genetic groups, but displayed increased levels from prepartum to d 6 and 21 postpartum. Our results indicated that expression in the liver of genes involved with glucose and fatty acid metabolism were similar in both groups of cows and significant differences were observed between the 2 groups in the expression of MUT, a gene involved in propionate metabolism.
from #MedicinebyAlexandrosSfakianakis via xlomafota13 on Inoreader http://ift.tt/2B0C6eL
via IFTTT
Αρχειοθήκη ιστολογίου
-
►
2020
(289)
- ► Φεβρουαρίου (28)
-
►
2019
(9071)
- ► Δεκεμβρίου (19)
- ► Σεπτεμβρίου (54)
- ► Φεβρουαρίου (3642)
- ► Ιανουαρίου (3200)
-
►
2018
(39872)
- ► Δεκεμβρίου (3318)
- ► Σεπτεμβρίου (3683)
- ► Φεβρουαρίου (2693)
- ► Ιανουαρίου (3198)
-
▼
2017
(41099)
- ► Δεκεμβρίου (3127)
-
▼
Νοεμβρίου
(6729)
-
▼
Νοε 22
(503)
- Cancer-testis gene PIWIL1 promotes cell proliferat...
- Prognostic value of PD-L1 expression in combinatio...
- Three cases of Nagashima-type palmoplantar keratos...
- Abrupt generalized pustules in patients with rheum...
- Unusual subcutaneous invasion of myxoid liposarcoma
- Transverse nasal crease with milia and comedones: ...
- Successful treatment of hidradenitis suppurativa w...
- Agranulocytosis associated with voriconazole-induc...
- Non-corticosteroid adherence and itch severity inf...
- Three cases of Nagashima-type palmoplantar keratos...
- Waardenburg syndrome type IIE in a Japanese patien...
- Abrupt generalized pustules in patients with rheum...
- Economic assessment of actual prescription of drug...
- Unusual subcutaneous invasion of myxoid liposarcoma
- Transverse nasal crease with milia and comedones: ...
- Successful treatment of hidradenitis suppurativa w...
- Agranulocytosis associated with voriconazole-induc...
- Core outcome research measures in anal cancer (COR...
- Maternal and neonatal outcomes of vaginal breech d...
- Impact of multimorbidity on healthcare professiona...
- Authorship, plagiarism and conflict of interest: v...
- Midwife or doctor local opinion leader to implemen...
- The effect of nut consumption on markers of inflam...
- Cerebrospinal fluid biomarkers for the diagnosis a...
- Local anaesthetics combined with vasoconstrictors ...
- Identifying clinical features in primary care elec...
- Effectiveness of Gastrostomy for Improving Nutriti...
- Effectiveness of Gastrostomy for Improving Nutriti...
- Phytochemical and pharmacological evaluation of et...
- In vitro cytotoxic activity of medicinal plants fr...
- A putative Chondroprotective role for IL-1β and MP...
- Acknowledgements to Reviewers
- Author Guidelines
- Biallelic mutations in NALCN: Expanding the genoty...
- Acknowledgements to Reviewers
- Nasal Cytology: a Precision Medicine tool in clini...
- The COL5A3 and MMP9 genes interact in eczema susce...
- Novel STRA6 null mutations in the original family ...
- Single suture craniosynostosis: Identification of ...
- Congenital limb deficiencies and major associated ...
- Nasal Cytology: a Precision Medicine tool in clini...
- Brain-derived neurotrophic factor (BDNF) determine...
- Relevance-dependent modulation of tactile suppress...
- The development of neuromotor skills and hand pref...
- Leaf saponins of Quillaja brasiliensis enhance lon...
- Molecular epidemiology of influenza B virus and im...
- Knowledge, attitudes, and practices of private sec...
- Genomic risk prediction of aromatase inhibitor-rel...
- Synergistic inhibition of cell proliferation by co...
- Synergistic inhibition of cell proliferation by co...
- Dose warping uncertainties for the accumulated rec...
- Reduction of MRI signal distortion from titanium i...
- Interdependent feedback regulation of breathing by...
- Direct Testing for Allele-Specific Expression Diff...
- Reference Assembly and Annotation of the Pyrenopho...
- Restorer-of-Fertility Mutations Recovered in Trans...
- Wnt/{beta}-catenin pathway activation mediates ada...
- A Novel YAP1 Inhibitor targets CSCs-enriched Radia...
- Mechanism informed repurposing of minocycline over...
- Inhibition of O-GlcNAcase sensitizes apoptosis and...
- The administration of surfactant decreased oxidati...
- Protocol for a systematic review and meta-analysis...
- Toxins, Vol. 9, Pages 378: A Supercluster of Neutr...
- Response Regarding Surgical Techniques for Retrogr...
- Feruloyl esterase from Alternaria tenuissima that ...
- Chemical leaching of copper-zinc concentrate with ...
- Oxylipins and oxylipin synthesis pathways in fungi
- Expression of the zebrafish β-defensin 3 mature pe...
- Antimicrobial potential of alkalophilic micromycet...
- Creation of thermostable polypeptide cassettes for...
- Effect of potassium sorbate, sodium benzoate, and ...
- Hydrogen peroxide-induced salt tolerance in the Ar...
- Activity of Na + /K + -ATPase and the content of p...
- Rational design of enzyme compositions for the pro...
- Specific features of chitosan depolymerization by ...
- Cultivation of the microalga Neochloris oleoabunda...
- Residual Convolutional Neural Network for Determin...
- Noise exposure while commuting in Toronto - a stud...
- Room Temperature Ni(0)/PCy3-Catalyzed Coupling Rea...
- Noise exposure while commuting in Toronto - a stud...
- The impact of spatio-temporal calcium dynamics wit...
- LEARNING-INDUCED MODULATION OF THE EFFECT OF ENDOC...
- The Modulation of Hippocampal Theta Rhythm by the ...
- Prefrontal cortical specific differences in behavi...
- Perceptual Decisions Based on Previously Learned I...
- A systematic literature review of evidence-based c...
- The humanistic burden of Pompe disease: are there ...
- Procalcitonin-guided antibiotic therapy in intensi...
- To infinity…. and beyond!!
- Understanding and prevention of orofacial clefting...
- Guided bone regeneration for the reconstruction of...
- Fast-track orthognathic surgery: An evidence-based...
- Unusual case of acquired capillary hemangioma of t...
- Multiple dentigerous cysts as a rare presentation ...
- Lugol chromoscopy in the follow-up of head and nec...
- Karapandzic flap for esthetic and functional recon...
- Evaluation of the sternoclavicular graft for the r...
- Dual pathology of mandible
- A randomized control trial of awake oral to submen...
- Efficacy of exclusive lingual nerve block versus c...
-
▼
Νοε 22
(503)
- ► Σεπτεμβρίου (2173)
-
►
2016
(13807)
- ► Δεκεμβρίου (700)
- ► Σεπτεμβρίου (600)
- ► Φεβρουαρίου (1350)
- ► Ιανουαρίου (1400)
-
►
2015
(1500)
- ► Δεκεμβρίου (1450)
Ετικέτες
Τετάρτη 22 Νοεμβρίου 2017
Expression of hepatic genes related to energy metabolism during the transition period of Holstein and F1 Holstein-Gir cows
Εγγραφή σε:
Σχόλια ανάρτησης (Atom)
Δεν υπάρχουν σχόλια:
Δημοσίευση σχολίου