The recent adaptation of CRISPR Cas9 genome editing to Leishmania spp. has opened a new era in deciphering Leishmania biology. The method was recently improved using a PCR-based CRISPR Cas9 approach, which eliminated the need for cloning. This new approach, which allows high-throughput gene deletion, was successfully validated in L. mexicana and L. major. In this study, we validated the toolkit in Leishmania donovani targeting the flagellar protein PF16, confirming that the tagged protein localizes to the flagellum and that null mutants lose their motility. We then used the technique to characterise CK1.1, a member of the casein kinase 1 family, which is involved in the regulation of many cellular processes. We showed that CK1.1 is a low-abundance protein present in promastigotes and in amastigotes. We demonstrated that CK1.1 is not essential for promastigote and axenic amastigote survival or for axenic amastigote differentiation, although it may have a role during stationary phase. Altogether, our data validate the use of PCR-based CRISPR Cas9 toolkit in L. donovani, which will be crucial for genetic modification of hamster-derived, disease-relevant parasites.
from #MedicinebyAlexandrosSfakianakis via xlomafota13 on Inoreader http://ift.tt/2AlFe84
via IFTTT
Αρχειοθήκη ιστολογίου
-
►
2020
(289)
- ► Φεβρουαρίου (28)
-
►
2019
(9071)
- ► Δεκεμβρίου (19)
- ► Σεπτεμβρίου (54)
- ► Φεβρουαρίου (3642)
- ► Ιανουαρίου (3200)
-
►
2018
(39872)
- ► Δεκεμβρίου (3318)
- ► Σεπτεμβρίου (3683)
- ► Φεβρουαρίου (2693)
- ► Ιανουαρίου (3198)
-
▼
2017
(41099)
- ► Δεκεμβρίου (3127)
-
▼
Νοεμβρίου
(6729)
-
▼
Νοε 29
(491)
- Respiratory sinus arrhythmia: Modeling longitudina...
- Effect of carpal tunnel release on median nerve ep...
- Medikamentöse Therapie neuroendokriner Neoplasien ...
- Medikamentöse Therapie neuroendokriner Neoplasien ...
- BranchAnalysis2D/3D automates morphometry analyses...
- Sequence-Specific DNA Recognition with Designed Pe...
- Hexafluoroisopropanol and Acetyl Chloride Promoted...
- Implementing noninvasive follicular thyroid neopla...
- Primary cutaneous mucoepidermal carcinoma
- Systemic thrombolysis in a patient with massive pu...
- Lyric hearing aid: a rare cause of benign necrotis...
- Primary cutaneous mucoepidermal carcinoma
- Lyric hearing aid: a rare cause of benign necrotis...
- Follicular bronchiolitis in an HIV-infected indivi...
- Cu6S6 clusters as a building block for the stabili...
- Relevance of genetic relationship in GWAS and geno...
- Randomized Split-face, Controlled Comparison of Tr...
- Implementing near real-time vaccine safety surveil...
- Editorial Board/Aims and Scope
- Attitudes about vaccines to prevent Ebola virus di...
- Ethics, pregnancy, and ZIKV vaccine research & dev...
- Randomized Split-face, Controlled Comparison of Tr...
- Identification of a novel compound targeting the n...
- Exercise Alters Gut Microbiota Composition and Fun...
- Adipose Lipolysis Unchanged by Preexercise Carbohy...
- Biological/Genetic Regulation of Physical Activity...
- Strength, Affect Regulation, and Subcortical Morph...
- Cardiometabolic Impact of Changing Sitting, Standi...
- Using voltage-sensor toxins and their molecular ta...
- SoftHand at the CYBATHLON: a user’s experience
- Anti-tumour necrosis factor-α antibodies and B cel...
- Chemical characterization of PM 2.5 collected from...
- Urine cell-free microRNA as biomarkers for transit...
- Social media usage among health care providers
- Experiencing physical warmth affects implicit atti...
- Operational research within the national tuberculo...
- Polymorphism rs13334190 in zinc finger protein 469...
- Acute Chest Syndrome: An Ongoing Challenge for Phy...
- Diet, Lung Function, and Asthma Exacerbations in P...
- Neutrophil–Lymphocyte Ratio in Children with Recur...
- Developing a Mobile Health Intervention for Low-In...
- Acute Chest Syndrome: An Ongoing Challenge for Phy...
- Diet, Lung Function, and Asthma Exacerbations in P...
- Neutrophil–Lymphocyte Ratio in Children with Recur...
- Developing a Mobile Health Intervention for Low-In...
- Epidemiology, biology and therapy of Merkel cell c...
- Chasing Seasonal Influenza — The Need for a Univer...
- Chasing Seasonal Influenza — The Need for a Univer...
- Ih Interacts with Somato-Dendritic Structure to De...
- Time course of functional recovery during first th...
- Clustering of Heading Selectivity and Perception-R...
- Complementary metrics of human auditory nerve func...
- Implantable computer-controlled adaptive multi-ele...
- Quantitative relations between BOLD responses, cor...
- Estimating properties of the fast and slow adaptiv...
- Quantitative input: output relationships between h...
- Effect of Sensory Attenuation on Cortical Movement...
- Modeling spatial navigation in the presence of dyn...
- Bayesian Diallel Analysis Reveals Mx1-Dependent an...
- Dissecting the Genetic Architecture of Shoot Growt...
- Dependency of Heterochromatin Domains on Replicati...
- Identifying Genetic Differences Between Dongxiang ...
- Enhanced Mechanical and Helical Properties with Ac...
- MicroRNA dynamics at the onset of primordial germ ...
- Outcomes of Children and Adolescents with Advanced...
- Premature birth affects the degree of airway dysan...
- Genotypic characterization of gentamicin and cepha...
- Prognostic Factors for Survival After Transarteria...
- The Study of Clear Cell Renal Cell Carcinoma with ...
- High-risk Plaque and Calcification Detected by Cor...
- Anti-inflammatory evaluation of the methanolic ext...
- Jia-Wei-Jiao-Tai-Wan ameliorates type 2 diabetes b...
- PTBP3-mediated regulation of ZEB1 mRNA stability p...
- Modeling the subclonal evolution of cancer cell po...
- HER2-driven Breast Tumorigenesis Relies upon Inter...
- LSR antibody therapy inhibits ovarian epithelial t...
- Photodynamic priming mitigates chemotherapeutic se...
- The E3 ligase RING1 targets p53 for degradation an...
- PPAR{delta} elicits ligand-independent repression ...
- Delivering type I interferon to dendritic cells em...
- Disparities in Prostate, Lung, Breast, and Colorec...
- Diagnostic accuracy of CTA and MRI/MRA in the eval...
- Determination of antimicrobial susceptibility patt...
- Identification of pathogens for differential diagn...
- Decision tree for accurate infection timing in ind...
- Daptomycin treatment in patients with resistant st...
- Which Preschool Children With Specific Language Im...
- Re-irradiation for recurrent glioma- the NCI exper...
- Interventions to increase adherence in patients ta...
- Decreasing Delirium through Music (DDM) in critica...
- Errata
- Errata
- Errata
- Irinotecan and Oxaliplatin Might Provide Equal Ben...
- Robotic-assisted Transperitoneal Infrarenal Para-a...
- Anastomotic Leakage Using Linear Stapling Device w...
- Association Between PD-L1 Expression and Metabolic...
- A Glissonean Approach with Individual Isolation Du...
- Mucinous Cystic Neoplasms Lined by Abundant Mucino...
- Outcome Evaluation of HER2 Breast Cancer Patients ...
-
▼
Νοε 29
(491)
- ► Σεπτεμβρίου (2173)
-
►
2016
(13807)
- ► Δεκεμβρίου (700)
- ► Σεπτεμβρίου (600)
- ► Φεβρουαρίου (1350)
- ► Ιανουαρίου (1400)
-
►
2015
(1500)
- ► Δεκεμβρίου (1450)
Ετικέτες
Τετάρτη 29 Νοεμβρίου 2017
Characterisation of Casein Kinase 1.1 in Leishmania donovani Using the CRISPR Cas9 Toolkit
Εγγραφή σε:
Σχόλια ανάρτησης (Atom)
Δεν υπάρχουν σχόλια:
Δημοσίευση σχολίου