pH changes of mixed biofilms of Streptococcus mutans and Candida albicans after exposure to sucrose solutions in vitro

Publication date: Available online 6 March 2018
Source:Archives of Oral Biology
Author(s): Thamires Priscila Cavazana, Juliano Pelim Pessan, Thayse Yumi Hosida, Douglas Roberto Monteiro, Alberto Carlos Botazzo Delbem
ObjectiveThis study aimed to standardize an in vitro experimental model able to reproduce the pH changes that occur in dental biofilm under in vivo conditions, using a mixed biofilm of Streptococcus mutans and Candida albicans.DesignBiofilms were developed for 96 hours, and exposed to three different concentrations of sucrose (10, 20 or 30%) during 1, 3 or 5 minutes. The pH was measured before exposure to sucrose, immediately after its removal from the biofilms, and at 1, 3, 5 and 10 minutes after removal.ResultsSucrose solutions at 10 and 20% required 1 minute to significantly reduce the biofilm pH, while for 30% sucrose a significant reduction was already seen immediately after its removal, even for the shortest exposure time. For an exposure of 3 minutes to 20% sucrose, the biofilm pH attained the critical value for hydroxyapatite dissolution when measured 1 minute after sucrose removal, followed by a recovery phase.ConclusionsA mixed biofilm of S. mutans and C. albicans exposed to a 20% sucrose solution for 3 minutes exhibited a pattern of pH change similar to that observed in vivo, despite at a higher speed when compared to in vivo conditions.



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