IJMS, Vol. 19, Pages 1150: Localization Microscopy of Actin Cytoskeleton in Human Platelets

IJMS, Vol. 19, Pages 1150: Localization Microscopy of Actin Cytoskeleton in Human Platelets

International Journal of Molecular Sciences doi: 10.3390/ijms19041150

Authors: Sandra Mayr Fabian Hauser Anja Peterbauer Andreas Tauscher Christoph Naderer Markus Axmann Birgit Plochberger Jaroslaw Jacak

Here, we measure the actin cytoskeleton arrangement of different morphological states of human platelets using a new protocol for photo-switching of rhodamine class fluorophores. A new medium composition was established for imaging the cytoskeleton using Alexa Fluor 488 conjugated to phalloidin. Morphological states of platelets bound to a glass substrate are visualized and quantified by two-dimensional localization microscopy at nanoscopic resolution. Marker-less drift correction yields localization of individual Alexa 488 conjugated to phalloidin with a positional accuracy of 12 nm.

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