Αρχειοθήκη ιστολογίου

Πέμπτη 7 Ιουλίου 2016

A prospective randomized trial of LigaSure Small Jaw® versus conventional neck dissection in head and neck cancer patients

Abstract

Objectives

We compared the differences between LigaSure Small Jaw®-assisted and conventional neck dissection in head and neck cancer patients.

Design

Prospective randomized study.

Setting

Tertiary referral hospital.

Participants

Patients scheduled to undergo neck dissection due to head and neck cancer were eligible for this study. The study group was treated using the LigaSure vessel sealing system (Small Jaw®; Covidien, Colorado, USA) for dissection and hemostasis throughout the whole procedures. (ClinicalTrials . gov number : NCT02597582).

Main outcomes measures

Operation duration, perioperative blood loss, postoperative drainage amount, and postoperative pain status.

Results

The study group consisted of 21 patients while the control group had 20 patients. The operation duration was shorter (97.1 versus 116.3 minutes, P = 0.022) and the average amount of injected analgesics was lower (8.8 versus 17.7 ampules, P = 0.037) in the study group.

Conclusions

The assistance of the LigaSure Small Jaw® during functional neck dissection shortened operation duration and decreased the amount of injected analgesics needed.

This article is protected by copyright. All rights reserved.



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Expressions of isopeptide bonds and corneodesmosin in middle ear cholesteatoma

Abstract

Objective

Isopeptide bonds form cross-links between constituent proteins in the horny layer of the epidermis. Corneodesmosin (CDSN) is a major component of corneodesmosomes, which bind corneocytes together. Both play important roles in maintaining epidermal barrier functions. In the present study, we investigated the expressions of isopeptide bonds, CDSN, and related enzymes in middle ear cholesteatoma in comparison with the skin.

Methods

Cholesteatoma and normal postauricular skin were collected from patients with acquired middle ear cholesteatoma during tympanomastoidectomy. Expression of ε-(γ-glutamyl)lysine isopeptide bonds was examined by immunohistochemistry; Expressions of transglutaminase (TGase)1, TGase2, TGase3, and TGase5 by immunohistochemistry and quantitative RT-PCR (qRT-PCR); expression of CDSN by immunohistochemistry, qRT-PCR, and Western blot; and expressions of tissue kallikrein-related peptidase (KLK)5, KLK7, KLK14, and serine peptidase inhibitor Kazal type 5 (SPINK5) by qRT-PCR.

Results

TGase2 was higher (P=0.0046) and TGase5 was lower (P=0.0008) in cholesteatoma than in the postauricular skin. Immunoreactivity for isopeptide bonds was localized in the granular and horny layers, and was not different between the two tissues. Immunoreactivity for CDSN was localized in the granular layer, and was lower in cholesteatoma than in the skin (P=0.0090). Western blot and qRT-PCR confirmed that the expression of CDSN was lower in cholesteatoma than in the skin. Expressions of KLK5, KLK7, KLK14, or SPINK5 were not different between the two tissues.

Conclusions

These results indicate that the production of CDSN is likely to be suppressed in cholesteatoma, which would account, at least in part, for the mechanical fragility and increased permeability of the cholesteatoma epithelium.

This article is protected by copyright. All rights reserved.



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Quiz: Where should you take your long-overdue vacation from the station?

Every EMT on this planet deserves a vacation. EMS is a stressful job. Whether you can actually take one is a different story.

It's OK to dream, though, and when the time comes for your long-overdue break, this quiz will show you exactly where you should take your next vacation from the station.

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Issue Information – Copyright



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Issue Information – Masthead



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Liganded thyroid hormone receptors transactivate the DNA methyltransferase 3a gene in mouse neuronal cells

Endocrinology, Early Release.


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Gq-protein-Coupled Membrane-Initiated Estrogen Signaling Rapidly Excites Corticotropin-Releasing Hormone Neurons in the Hypothalamic Paraventricular Nucleus in Female Mice

Endocrinology, Early Release.


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Analysis of monosomy-3 in immunomagnetically-isolated circulating melanoma cells in uveal melanoma patients

Summary

Monosomy-3 in primary uveal melanoma (UM) is associated with a high risk of metastasis and mortality. Although circulating melanoma cells (CMC) can be found in most UM patients, only approximately 50% of the patients develop metastases. We utilized a novel Immuno-FISH assay to detect chromosome-3 in intact CMC isolated by dual-immunomagnetic enrichment. CMC were detected in 91% of the patients (n=44) with primary non-metastatic UM, of which 58% were positive for monosomy-3. The monosomy-3 status of CMC corresponded to the monosomy-3 status of the primary tumor in 10 of the 11 patients where this could be tested. Monosomy-3 in the CMC was associated with an advanced tumor stage (P = 0.046) and was detected in all four patients who developed metastasis within the follow-up period of 4 years. This non-invasive technique may enable the identification of UM patients at risk for metastasis particularly when a primary tumor specimen is unavailable.

This article is protected by copyright. All rights reserved.



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Loss of strumpellin in the melanocytic lineage impairs the WASH Complex but does not affect coat colour

Abstract

The five-subunit WASH complex generates actin networks that participate in endocytic trafficking, migration and invasion in various cell types. Loss of one of the two subunits WASH or strumpellin in mice is lethal but little is known about their role in mammals in vivo. We explored the role of strumpellin, which has previously been linked to hereditary spastic paraplegia, in the mouse melanocytic lineage. Strumpellin knockout in melanocytes revealed abnormal endocytic vesicle morphology but no impairment of migration in vitro or in vivo and no change in coat colour. Unexpectedly, WASH and filamentous actin could still localise to vesicles in the absence of strumpellin, although the shape and size of vesicles was altered. Blue Native-PAGE revealed the presence of two distinct WASH complexes, even in strumpellin knockout cells, revealing that the WASH complex can assemble and localise to endocytic compartments in cells in the absence of strumpellin.

This article is protected by copyright. All rights reserved.



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A phase II trial comparing pazopanib with doxorubicin as first-line treatment in elderly patients with metastatic or advanced soft tissue sarcoma (EPAZ): study protocol for a randomized controlled trial

Anthracycline-based treatment remains the backbone of chemotherapy for nonresectable soft tissue sarcomas (STS). More than 30 % of patients with STS are aged 60 years or older, limiting the choice of treatment...

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Kanamycin inhibits daidzein metabolism and abilities of the metabolites to prevent bone loss in ovariectomized mice

Daidzein is an isoflavone derived from soybeans that exerts preventive effects on bone loss in ovariectomized (OVX) animals. These effects have been correlated with increasing serum equol levels. In the presen...

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Frequent detection of human polyomavirus 6 in keratoacanthomas

The recent discovery of the Merkel cell polyomavirus and its consistent association with Merkel cell carcinoma has drawn attention to the numerous recently discovered polyomaviruses and their possible involvem...

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Long-term survival of early-stage non–small cell lung cancer patients who underwent robotic procedure: a propensity score-matched study

Abstract

Background

In the past decade, many researchers focused on to robot-assisted surgery. However, on long-term outcomes for patients with early-stage non–small cell lung cancer (NSCLC), whether the robotic procedure is superior to video-assisted thoracic surgery (VATS) and thoracotomy is unclear. Nonetheless, in the article titled "Long-term survival based on the surgical approach to lobectomy for clinical stage I non–small cell lung cancer: comparison of robotic, video assisted thoracic surgery, and thoracotomy lobectomy" by Yang et al. that was recently published in Annals of Surgery, the authors provided convincing evidence that the robotic procedure results in similar long-term survival as compared with VATS and thoracotomy. Minimally invasive procedures typically result in shorter lengths of hospital stay, and the robotic procedure in particular results in superior lymph node assessment.

Main body

Our propensity score-matched study generated high-quality data. Based on our findings, we see promise in expanding patient access to robotic lung resections. In this study, propensity score matching minimized the bias involved between groups. Nevertheless, due to its retrospective nature, bias may still exist. Currently, the concept of rapid rehabilitation is widely accepted, and it is very difficult to set up a randomized controlled trial to compare robotic, VATS, and thoracotomy procedures for the treatment of NSCLC. Therefore, to overcome this limitation and to minimize bias, the best approach is to use a registry and prospectively collected, propensity score-matched data.

Conclusions

Robotic lung resections result in similar long-term survival as compared with VATS and thoracotomy. Robot-assisted and VATS procedures are associated with short lengths of hospital stay, and the robotic procedure in particular results in superior lymph node assessment. Considering the alarming increase in the incidence of lung cancer in China, a nationwide database of prospectively collected data available for clinical research would be especially important.



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Outcomes of preoperative chemoradiotherapy followed by surgery in patients with unresectable locally advanced sigmoid colon cancer

Abstract

Background

Complete resection of locally advanced sigmoid colon cancer (LASCC) is sometimes difficult. Patients with LASCC have a dismal prognosis and poor quality of life, which has encouraged the evaluation of alternative multimodality treatments. This prospective study aimed to assess the feasibility and efficacy of neoadjuvant chemoradiotherapy (neoCRT) followed by surgery as treatment of selected patients with unresectable LASCC.

Methods

We studied the patients with unresectable LASCC who received neoCRT followed by surgery between October 2010 and December 2012. The neoadjuvant regimen consisted of external-beam radiotherapy to 50 Gy and capecitabine-based chemotherapy every 3 weeks. Surgery was scheduled 6–8 weeks after radiotherapy.

Results

Twenty-one patients were included in this study. The median follow-up was 42 months (range, 17–57 months). All patients completed neoCRT and surgery. Resection with microscopically negative margins (R0 resection) was achieved in 20 patients (95.2%). Pathologic complete response was observed in 8 patients (38.1%). Multivisceral resection was necessary in only 7 patients (33.3%). Two patients (9.5%) experienced grade 2 postoperative complications. No patients died within 30 days after surgery. For 18 patients with pathologic M0 (ypM0) disease, the cumulative probability of 3-year local recurrence-free survival, disease-free survival and overall survival was 100.0%, 88.9% and 100.0%, respectively. For all 21 patients, the cumulative probability of 3-year overall survival was 95.2% and bladder function was well preserved.

Conclusion

For patients with unresectable LASCC, preoperative chemoradiotherapy and surgery can be performed safely and may result in an increased survival rate.



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Erratum: Prevalence of airflow obstruction among ever-employed US adults aged 18-79 years by longest held occupation group: National Health and Nutrition Examination Survey 2007-2010

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Kurth L, Doney B, Halldin C. Short Report: Prevalence of airflow obstruction among ever-employed US adults aged 18–79 years by longest held occupation group: National Health and Nutrition Examination Survey 2007–2010. Occup Environ Med 2016;73:482–6.

The title that reads: "Prevalence of airflow obstruction among ever-employed US adults aged 18–79 years by longest held occupation group: National Health and Nutrition Examination Survey 2007–2010" should read "Prevalence of airflow obstruction among ever-employed US adults aged 18–79 years by longest held occupation group: National Health and Nutrition Examination Survey 2007–2008".

All references on page 482 to 'the 2007–2010 National Health and Nutrition Examination Survey (NHANES)' and/or '2007–2010 NHANES data' should read 'the 2007–2008 National Health and Nutrition Examination Survey (NHANES)' and '2007–2008 NHANES data'.

The sentences on page 482 that read "The US population, 18–79 years, was studied using NHANES data from the combined cross-sectional 2007–2008 and 2009–2010 survey cycles. These were the most current NHANES...



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Non-Certified Firefighter/EMT Trainee - Marion County Fire Rescue

MARION COUNTY FIRE RESCUE 2631 SE 3RD STREET OCALA, FLORIDA 34471 Updated: July 05, 2016 Classification: Non-Certified Firefighter/EMT Trainee Marion County Fire Rescue will be conducting a hiring process for 22 Non-Certified Firefighter/EMTs; job posting opens July 1, 2016 and will remain open until August 5, 2016. Testing is conducted through National Testing Network (NTN). Work Schedule and Starting ...

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EMS leader’s 8-step guide to excellent pediatric care

By Sean Caffrey, NEMSMA

It is easy to find a wealth of educational material on EMS pediatric care despite the fact that most children encountered by EMS are not seriously ill or injured. How is it then that kids are a small fraction, 5 to 10 percent, of EMS patients, but command so much attention?

The reason is that effectively managing pediatric patients, including the rarely encountered critically ill pediatric patients, requires good preparation, ongoing training and comprehensive oversight by EMS services.

While the knowledge and equipment needed to take care of kids may be specialized, this should not suggest that EMS responders or response organizations are not equipped to handle sick kids.

You have likely heard "children are not small adults." While this line is certainly catchy, there is significant debate about its value within the pediatric emergency medicine community since it seems to imply that providers who take care of adults may not be skilled with children.

To the contrary, providing good care for children follows the same basic care principles as adult patients. Therefore, a more appropriate line might be "kids are patients, too," since they deserve the same level of assessment and care provided to an adult.

There are certainly some differences and special considerations, but nothing a skilled EMS practitioner can't handle. So how can leaders build these pediatric care skills for their teams?

1. Know frequent pediatric call types
The first preparatory step is knowing what to expect. The most frequent chief complaints for younger children include respiratory distress and seizures. For older children and adolescents, traumatic injury and behavioral or psychiatric complaints are the most common reasons for EMS activation.

What is most interesting, however, is that these are not the critical call types practitioners prepare for in a PALS class, nor are these the scenarios for which length-based resuscitation tapes were designed to handle.

As such, the forward-thinking EMS leader should examine their agency-specific call data to ensure that providers have the appropriate supplemental training to be well prepared for what likely constitutes the vast majority of their pediatric call volume.

2. Offline medical direction from evidence-based guidelines
Another important preparatory step is securing offline medical direction based on protocols reflecting current standards of pediatric care. Within the last few years, a number of evidenced-based guidelines have been published to assist medical directors and administrators in this area.

These pediatric EBGs represent a methodical approach to evaluating existing evidence in order to build the most effective care guidelines. While not protocols themselves, EBGs are great tools to use when building protocols and have already been implemented in a number of states.

Currently published guidelines include pediatric seizure management, as well as pain control for traumatic injuries. A respiratory distress guideline is also expected soon. Share these links and resources with your EMS medical director.

3. Medical direction from a pediatric specialist
A number of progressive services across the country, as well as a couple of states, have begun to add associate EMS medical directors for pediatric care. This trend primarily occurs in EMS services with ready access to children's hospitals. However, it may be worth contacting your regional children's hospital to see if their pediatric emergency medicine specialists are willing to participate in your medical direction system, or at least review your department's pediatric protocols.

I work closely with pediatric emergency medicine physicians and can assure you that it is great to have that level of expertise available as a resource to EMS practitioners. It is equally valuable to introduce these specialists, who often have a limited opportunity to interact outside their institutions, to EMS services throughout their region.

While it may take some effort to make this connection, you might be amazed what happens if you ask.

4. Providers need pediatric-specific tools
A final step in offline medical direction is making sure your practitioners have good tools available to implement their protocols. The most important tool is a product or method to estimate patient weight and determine drug dosages.

A pediatric drug quick-reference guide to determine fluid and drug dosage calculations needs to include information for commonly used respiratory, anti-seizure and pain medications. Many systems use pocket cards, quick-reference books, charts or apps in addition to length-based tapes that focus on resuscitation.

Last, a frequently overlooked tool is one to measure pain. As many younger children cannot use a standard zero-to-10 pain scale. Having access to a Faces, Legs, Activity, Cry and Consolability and a Wong-Baker Faces Scale are important to be able to treat pediatric pain or traumatic injuries effectively.

5. Essential pediatric equipment
Most states have minimum requirements for pediatric equipment based on nationally recommended equipment lists. As part of the work of the National EMS for Children program, your service has likely been surveyed regarding the availability of this equipment.

EMS leaders should strongly consider adding the following items:

  • A set-up for pull-push fluid administration. According to the most recent sepsis and shock guidelines, children in shock should receive 20 mL/kg in the first 5 to 10 minutes. Fluid resuscitation goals are to achieve normal vital signs within the first hour of shock presentation. Infusions at this rate are simply not possible using the unregulated administration of fluid through an intravenous bag alone or through a burette system. Services should consider carrying a three-way stopcock device and 60-cc Luer lock tip syringes that can be used to quickly and accurately administer fluid during resuscitation.
  • Diagnostic equipment to assess blood pressure and pulse oximetry. This includes appropriately sized blood pressure cuffs and pulse oximetry probes. In addition, automatic blood pressure cuffs, which are essential in obtaining a blood pressure on infants and toddlers, should be strongly considered. Previous teaching that blood pressure measurement is unimportant in children should be disregarded, as this vital sign is as critical to effective assessment and care of children as it is in adults.
  • Mushroom-tip or BBG type suction catheters are significantly more effective than bulb syringes or traditional Yankauer rigid suction tips at removing nasal secretions, especially in young children unable to blow their noses to alleviate respiratory distress. Such a device is easier to use and less traumatic, and does not risk stimulation of a vagal response.
  • Appropriate distraction and trust-building tools such as stuffed animals or search-and-find distraction books can assist children in coping with the EMS encounter.  

6. Delivering pediatric care
When delivering pediatric care, it is important to consider that the most common problem with the care of children is the failure to deliver appropriate care when indicated. In some instances, practitioners may talk themselves out of essential interventions due to inadequate assessment or fear of agitating a child.

Examples of this include not obtaining vital signs and withholding essential respiratory, fluid resuscitation, glucose, pain control or spinal motion restriction. The best method to address these issues is to ensure a complete assessment, including a blood pressure, pulse oximetry, glucose measurement, pain measurement and capnography on all seriously ill children.

Simulation training improves care through practice with your service's protocols, reference materials, diagnostic tools and pediatric equipment. This is especially important considering the low volume of pediatric EMS encounters.

7. Assign a pediatric care champion
Make someone at your service responsible for preparation, equipment and training issues. In the most recent national Pediatric Readiness Assessment, over 4,000 hospital emergency department representatives across the United States were asked if they assigned a nurse or physician to the role of a pediatric care coordinator or champion to oversee pediatric readiness at their facilities.

The facilities that indicated such a role existed were found to score significantly higher on their overall readiness scores [1]. Assigning this role to an aspiring and motivated practitioner or supervisor in your organization could be just as helpful to your overall pediatric readiness.

8. Measure success
Children are a specialized patient population that require additional effort. Comprehensive review of your organization's pediatric calls is critical. Use your own electronic records, which do a great job of describing what types of patients you encounter and how well care is delivered to them.

Since critically ill children are a rare occurrence in any EMS system, the ability to evaluate and communicate findings about the care delivered on these calls, if done in an effective and non-punitive manner, will provide the opportunity for all of your practitioners to learn from these rare experiences. As such, quality improvement personnel should be sure to develop guidelines to regularly review both high-acuity and a subset of low-acuity pediatric calls.

Set up for future performance measurement success
The National EMS for Children program is in the process of approving new EMS performance measures for EMS for Children state partnerships to implement. Those measures will likely include electronic patient care reporting to the states on the NEMSIS version 3 standard, establishment of pediatric care coordinators in EMS services and competency testing of providers.

If your service implements these eight steps, your department will be well positioned to meet or exceed the expectations of these performance measures and provide great care for kids in the process.    

About the author:
Sean Caffrey, MBA, CEMSO, NRP currently serves at the EMS Programs Manager for the University of Colorado School of Medicine, Pediatric Emergency Medicine Section. Sean has been certified as a paramedic since 1991and has worked in volunteer, private, hospital-based, fire-based and 3rd service EMS systems in roles from provider through department head. He currently works in conjunction with the state EMS office in Colorado, is the vice president of the EMS Association of Colorado, is a board member of the National EMS Management Association, and a member of NAEMT, NASEMSO and NAEMSP. Sean's interests include EMS system design, pediatrics, public policy, professional development and research. 

Reference:

1. Gausche-Hill M, Ely M, Schmuhl P, et al. A National Assessment of Pediatric Readiness of Emergency Departments. JAMA Pediatr. 2015;169(6):527-534. doi:10.1001/jamapediatrics.2015.138.

 



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Deriving gradient measures of child speech from crowdsourced ratings

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Publication date: Available online 6 July 2016
Source:Journal of Communication Disorders
Author(s): Tara McAllister Byun, Daphna Harel, Peter F. Halpin, Daniel Szeredi
Recent research has demonstrated that perceptual ratings aggregated across multiple non-expert listeners can reveal gradient degrees of covert contrast between target and error sounds that listeners might transcribe identically. Aggregated ratings have been found to correlate strongly with acoustic gold standard measures both when individual raters use a continuous rating scale such as visual analog scaling (Munson et al., 2012) and when individual raters provide binary ratings (McAllister Byun et al., 2015). In light of evidence that inexperienced listeners use continuous scales less consistently than experienced listeners, this study investigated the relative merits of binary versus continuous rating scales when aggregating responses over large numbers of naive listeners recruited through online crowdsourcing. Stimuli were words produced by children in treatment for misarticulation of North American English/r/. Each listener rated the same 40 tokens two times: once using Visual Analog Scaling (VAS) and once using a binary rating scale. The gradient rhoticity of each item was then estimated using (a) VAS click location, averaged across raters; (b) the proportion of raters who assigned the "correct/r/" label to each item in the binary rating task (pˆ). First, we validate these two measures of rhoticity against each other and against an acoustic gold standard. Second, we explore the range of variability in individual response patterns that underlie these group-level data. Third, we integrate statistical, theoretical, and practical considerations to offer guidelines for determining which measure to use in a given situation.



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Diversity and Activity of Communities Inhabiting Plastic Debris in the North Pacific Gyre

Marine plastic debris is a growing concern that has captured the general public's attention. While the negative impacts of plastic debris on oceanic macrobiota, including mammals and birds, are well documented, little is known about its influence on smaller marine residents, including microbes t...

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Environmental disturbances decrease the variability of microbial populations

A central pursuit of microbial ecology is to accurately model changes in microbial community composition in response to environmental factors. This goal requires a thorough understanding of the drivers of variability in microbial populations. However, most microbial ecology studies focus on the ...

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E. coli: The ideal transport for next-gen vaccines?

BUFFALO, N.Y. - Most people recoil at the thought of ingesting E. coli. But what if the headline-grabbing bacteria could be used to fight disease?

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Facilitators of Survivorship Care Among Underserved Breast Cancer Survivors: a Qualitative Study

Abstract

Research investigating facilitators of survivorship care among underserved breast cancer survivors (BCS) is sparse. This study aimed to explore facilitators of survivorship care among underserved BCS within the first 5 years following chemotherapy, radiation, or surgery for breast cancer. In-depth interviews were conducted, using a semi-structured interview guide, with underserved BCS exploring survivorship care experiences. Content analysis of the verbatim transcripts was applied, and results were summarized according to themes related to facilitators of breast cancer survivorship care. Interviews were conducted with 25 BCS. Eight main themes were identified: coordination of care; positive perceptions of health care providers; communication between patient and health care providers; financial and insurance facilitators; information, classes, and programs provided; assistance provided by organizations and health care professionals; transportation facilitators; and job flexibility. This study provides a comprehensive look at facilitators of survivorship care among underserved BCS. BCS endorsed several facilitators of their survivorship care, mainly at the interpersonal, organizational, and societal level. This study adds to the research literature on catalysts of care among underserved BCS. Results from this study are currently being used to inform a patient navigation intervention to facilitate care among this population.



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A Modified Butterfly Graft Technique for Treating Nasal Obstruction

This case series describes the functional and aesthetic outcomes of patients with nasal obstruction who were managed with a butterfly graft technique modified to reduce graft width and visibility.

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Lower Eyelid Position Change After Skin-Muscle Flap Blepharoplasty

This case series of patients from a private facial plastic surgery practice describes change in lower eyelid position after transcutaneous lower eyelid blepharoplasty.

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The World’s Most Powerful Pair of Glasses—Part 3 of 3

If you have tuned in the last two weeks, you must be suitably impressed by Bailey the beluga. Not only can this and other whales "see" using sound, they can identify the size, shape, and texture of reflectors based on the nature of the reflections of sound from them. These animals pull of a second impressive physiological feat by controlling the gain of their auditory system to protect it from the loud echolocating clicks when they are produced but then rapidly increasing gain to receive the reflection.



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Heat and moisture exchanger use reduces in-hospital complications following total laryngectomy: a case–control study

Total laryngectomy (TL) is an appropriate oncologic operation for many patients with laryngeal cancer delivering excellent oncologic outcomes, however it remains beset with significant functional consequences....

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Bogotá, Colombia – A Study of Medtech Contrasts

Bogota
Chances are, when you think of the country of Colombia, the two "C's" come to mind: cocaine and crime. While this may have been true 30 years ago, the country has come a long ways in terms of eliminating drug and crime issues and shedding its bad reputation. Just a couple weeks ago, the Colombian government signed a ceacefire treaty with the Revolutionary Armed Forces of Colombia (FARC), a guerrilla organization, paving the way for a peace treaty and stability in the country.

Invest in Bogotá, a public/private organization dedicated to helping existing businesses with promotion and exposure within the capital city, as well as encouraging new national and international companies to set up shop in Bogotá, invited us on a press tour of the city to learn more about its healthcare sector. Starting at the Meditech Conference that was going on in the city's convention center during our visit, we circled around the city over three days, visiting various device companies, medical foundations, and clinics.

The current medical scene in Bogotá (and the entire country) that we witnessed was an interesting mix of both high- and low-tech, highlighting the difficulties and triumphs within the healthcare industry.

Bogota-production-facility
One of our first stops was to the Cirec Foundation, a clinic that provides prosthetic limbs to the many Colombians who cannot afford them. As we toured the facility, we observed how each prosthetic arm and leg was hand-crafted using techniques that would probably be considered outdated in some countries. But we also heard stories of the many patients, namely children whose limbs were blown off by old landmines or amputated as a result of falls into illegal mining pits, who have been served by Cirec. We also learned about Cirec's work with Carlos Arturo Torres, a Colombian industrial designer who developed an award-winning Lego-based prosthetic limb called the IKO.

EditorDespite the difficulties Colombians have to face in terms of healthcare, the country has actually long been a pioneer in medical innovation. During our visit to the Shaio Clinic with its emphasis on cardiology, we had the opportunity to meet Dr. Jorge Reynolds, who played a major role in realizing artificial cardiac pacemakers.  His pacemaker was literally a car battery and vacuum tubes to regulate the voltage and timing, connected to the inside of the patient's chest with a few very large wires. Though 80 years young, Dr. Reynolds directs research at the Shaio Clinic and has recently developed a self-powered "nano-pacemaker" smaller than a grain of rice, and will be starting clinical trials of it in just a matter of months. His also is involved in the development of a digital stethoscope that utilizes the cloud to assist with the diagnosis of heart sounds.

Another medical pioneer we met was Dr. Carmen Barraquer, of the Barraquer ophthalmology schools and clinics in Barcelona, Spain and Bogotá, and one of the foremost experts in the field of refractive vision correction. Throughout the 1940's-50's, her father, Jose Barraquer, developed the surgical technique that would evolve into LASIK and related vision correction surgeries. Today, the Barraquer Clinic in Bogotá constantly receives the most advanced ophthalmological devices and utilizes new, cutting edge surgical procedures. We were told that Dr. Barraquer, who is in her 70's, still receives laser vision correction systems direct from manufacturers in Germany for guidance and testing before they are released for sale.

Insimed
One final example of advanced medical technology in Bogotá was Insimed, a training center for physicians that uses the latest in medical simulation technology. Much like Stanford University's medical simulation center that we visited a few years ago, Insimed's training approach uses high-tech mannequins, laparoscopic and endoscopic simulators, and even a daVinci robotic surgery trainer. The didactic simulations also include additional discussion and feedback in one of Insimed's connected conference rooms. According to Insimed's director, the center is the only fully independent training center in the Americas, and Colombia's location and low cost makes it attractive to physicians from not only Latin America, but even from the U.S. and all around the world.

Our tour of Bogota ended, appropriately, in the emergency department of San Ignacio University Hospital. There, we witnessed the reality of Colombian healthcare; overcrowded halls and extremely long wait times reminded us that we were still in a developing country. But even in the hustle and bustle of the ER, the hospital staff prided themselves in a specially made waiting room chair that could recline, hold an IV drip, and accommodate a meal tray to make the long stay just a little more bearable.

In conclusion, Colombia has a very pioneering spirit when it comes to medical technology. Despite some lack of resources as a still developing nation, an imperfect healthcare system, and a not-so-positive reputation based on its checkered history, Colombians have found a way to make things work, and in some cases, have shown themselves to be ahead of the curve in medical technology. With numerous tax incentives, a strategic location, and a friendly culture with a low cost of living, the government is hoping to make Bogotá the capital of medical technology in Latin America. If the country continues to make as much progress in the next 20 years as they have in the past 20, we think it's certainly possible.

Scott would like to thank Ligia Escudero, David Canal, Juan Sebastián Pérez, and the rest of the Invest in Bogota team for hosting!

This post Bogotá, Colombia – A Study of Medtech Contrasts appeared first on Medgadget.

Medgadget?d=yIl2AUoC8zA Medgadget?d=qj6IDK7rITs Medgadget?i=jJIiKUErjrA:ru5IE7I50AM:gIN9


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Correction: Total viable bacterial count using a real time all-fibre spectroscopic system

Analyst, 2016, Advance Article
DOI: 10.1039/C6AN90038K, Correction
Open Access Open Access
Creative Commons Licence  This article is licensed under a Creative Commons Attribution 3.0 Unported Licence.
E. Bogomolny
To cite this article before page numbers are assigned, use the DOI form of citation above.
The content of this RSS Feed (c) The Royal Society of Chemistry


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Combined endovascular coiling and intra-aneurysmal allogeneic mesenchymal stromal cell therapy for intracranial aneurysms in a rabbit model: a proof-of-concept study

Objective

To assess the feasibility and efficacy of clinically translatable adjuvant mesenchymal stem/stromal cells (MSCs) therapy in improving the healing of coiled aneurysms in a rabbit elastase aneurysm model.

Methods

Bone marrow-derived MSC populations were isolated from three rabbit donors in a serum-free environment and independently characterized to confirm their identity. Elastase-induced carotid aneurysms were created in nine New Zealand white rabbits. Each animal received one of the following treatments based on previous randomization: (1) coiling alone (control group); (2) coiling with an intra-aneurysmal injection of saline (vehicle group); and (3) coiling with an intra-aneurysmal injection of 5 million allogeneic MSCs (treatment group). The animals were followed for 4 weeks post-treatment, at the end of which blinded analyses of angiograms and histology were performed.

Results

Histological results in the treatment group showed improvements over the control and vehicle groups, although the improvement over the vehicle group was not significant. Intra-aneurysmal cell therapy with 5 million allogeneic MSCs did not result in any major adverse events. Angiographic results did not show any significant difference among groups.

Conclusions

This proof-of-concept study shows that adjuvant MSC therapy for intracranial aneurysms is feasible and may enhance histological improvement of coiled aneurysms at 4 weeks post-treatment.



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Factors associated with perforator stroke after selective basilar artery angioplasty or stenting

Background and purpose

Perforator stroke is one of the most common complications of elective intracranial angioplasty and/or stenting, particularly in the basilar artery. Factors associated with the risk of post-procedural perforator stroke remain unexplored. We investigated factors affecting the risk of perforator stroke after basilar artery angioplasty and/or stenting.

Materials and methods

Consecutive patients undergoing basilar artery angioplasty and/or stenting due to symptomatic atherosclerotic stenosis were retrospectively included in this single-center study. Analyzed variables including demographic data, risk factors of atherosclerosis, symptoms, characteristics of imaging, and procedure factors were extracted from electronic health records or imaging data. The main outcome was perforator stroke associated with the procedure. Multivariate analysis that correlated factors with the occurrence of perforator stroke in these patients was performed.

Results

A total of 255 patients were included in the study. Perforator stroke associated with angioplasty and/or stenting was identified in 13 patients (5.1%). Variables with significant correlation with post-procedural perforator stroke included diabetes (OR 6.496; 95% CI 1.741 to 24.241; p=0.005), time from last symptom to procedure <18 days (OR 5.669; 95% CI 1.174 to 27.371; p=0.031), and pre-procedure stenosis percentage <88.4% (OR 5.882; 95% CI 1.465 to 23.608; p=0.012).

Conclusions

Diabetes, time from last symptom to procedure, and pre-procedure stenosis percentage may be factors affecting the risk of perforator stroke associated with basilar artery angioplasty and/or stenting. These factors should be considered in planning of potential basilar artery angioplasty and/or stenting and prospectively evaluated in future multicenter trials.



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5 costly EMS documentation mistakes

Here are the five EMS documentation mistakes that deny patients coverage they deserve and EMS agencies fair compensation for their services

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Heat and moisture exchanger use reduces in-hospital complications following total laryngectomy: a case–control study

Abstract

Background

Total laryngectomy (TL) is an appropriate oncologic operation for many patients with laryngeal cancer delivering excellent oncologic outcomes, however it remains beset with significant functional consequences. Following TL, the upper and lower airways are permanently disconnected, which causes unfiltered, cold air with reduced humidity to enter the tracheobronchial tree, resulting in mucus overproduction and an increase in the viscosity of the mucus. In response to this, Heat and moisture exchangers were developed to compensate for the lost functions of the upper respiratory tract and their effect on the patients' respiratory performance in addition to their quality of life.

Methods

The case records of 48 patients undergoing total laryngectomy were reviewed and data concerning demographics, surgical details, post-operative care requirements and adverse events was retrieved. Post hoc analysis of the case patients was undertaken to identify any benefit of using a heat and moisture exchanger (HME) system with particular reference to post-operative respiratory outcomes.

Results

There was no significant difference between case and control subjects based on demographics, extent of surgery or need for flap repair. 16 patients had used a HME and 32 patients had used external humidification (EH). Of those experiencing mucous plugging, only 3/24 (12.5 %) had used a HME system, in contrast to 21/24 (87.5 %) who used EH (Chi square = 9.375, p = 0.002). The odds ratio of having an adverse event if not using HME was 8.27 (CI = 1.94 – 35.71). Use of HME also significantly reduced the number of days requiring physiotherapy (1.75 days vs. 3.20 days, p = 0.034).

Conclusion

Use of an HME system can reduce in-hospital complications, in particular episodes of mucus plugging, and post-operative care requirements. Furthermore, there is a cost benefit to using HME systems that warrants more widespread introduction of these devices in head and neck surgery centers.



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Asynchronous bilateral anastomosis site sigmoid colon cancer after ureterosigmoidostomy: a case report

Abstract

Background

We present a case of asynchronously occurring adenocarcinomas 29 and 36 years after ureterosigmoidostomy for bladder cancer, respectively, at both anastomosis sites.

Case presentation

A colonoscopy that was performed on a 69-year-old man because of bloody stool and an elevated carcinoembryonic antigen (CEA) level revealed a polypoid lesion at the right ureterosigmoid anastomosis site 29 years after the patient's ureterosigmoidostomy. Endoscopic resection was performed, and the lesion was diagnosed as adenocarcinoma. Seven years later (36 years after ureterosigmoidostomy), an elevated lesion was detected at the left ureterosigmoid anastomosis site by colonoscopy performed after detection of high CEA levels. Biopsy revealed an adenocarcinoma that was immunohistologically positive for CDX2; sigmoidectomy and ureterectomy were subsequently performed. The pathological diagnosis of the second tumor was adenocarcinoma arising in the ureterosigmoid anastomosis site and invading the left ureter.

Conclusions

Diligent long-term follow-up of patients who underwent ureterosigmoidostomy is essential.



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5 costly EMS documentation mistakes

By Steve Johnson

One of the most frustrating scenarios for EMS agencies is the denial of coverage for ambulance transport for far too many patients.

In the vast majority of cases our clients share with us, the primary reason patients are deprived of coverage is not that ambulance transport was medically unnecessary. Rather, it's that some crew members either don't understand or simply don't care about their professional responsibility to carefully and completely document the patient's condition at the time of transport on their patient care report.

Most simply, EMS providers need to stop using vague, meaningless words, conclusory statements and phrases that do not accurately convey — in appropriate clinical terms —the true condition of the patient at the time of service.

Here are the five most common EMS documentation mistakes we see and how EMS providers can stop making these costly mistakes.

1. Facts surrounding the dispatch undocumented
Many times when an ambulance responds to a 911 call, that simple fact is missing from the PCR. And in way too many chart reviews or audits, we find no dispatch determinants or other clear indication of the patient's reported condition at the time of dispatch.

Dispatch information, including the patient's reported condition at the time of dispatch, has been a critical component of good quality patient care documentation since 2002. How is it that so many organizations still don't have this critical piece of their patient care clearly and consistently documented on the PCR some 14 years later"

Organizations, whether their dispatch is internal or external, need to ensure that they have dispatch protocols, approved by their medical director, that are clearly understood at all levels of the organization. Dispatchers then must clearly communicate to the crews the patient's reported condition, which crews must clearly document on their PCR.

2. Insufficient narrative of the patient's condition at the time of transport
Far too frequently we see PCR narratives that do little more than state where the patient was picked up from, where they were delivered to and some statement that indicates that the crew left the patient no worse off than they found them — such as, "patient transported without incident."

This is especially true in the case of non-emergency transports.

For every transport, whether emergency or non-emergency, the PCR narrative must state the facts accurately, objectively and completely so that the reader can answer the question: Was transport of this patient by means other an ambulance contraindicated"

Other questions that should never go unanswered for the reader include the patient's mobility status, as well as the patient's ability to assist with the transfer to and from the stretcher and how that transfer was accomplished-and why. The PCR narrative must also answer how the patient's ability to care for themself compares to the patient's normal condition.

Also answer, what prompted the patient to call for an ambulance" When did the patient's problems start" How have the patient's symptoms evolved"

We suggest that ambulance services obtain and crew members read, whenever possible, hospital admission summaries for the patients they transport.

That doesn't have to be all the tests that were performed and the final diagnosis, but simply the history of present illness and summary of the patient's condition upon arrival. These admission summaries will often provide concrete examples of how professional medical records are expected to be written and the information that may be missing from their PCR for that same patient.

Many times if crews had taken the time to understand their patient's presentation, and documented those findings, the ambulance service would have far less problems verifying and supporting the care they provided when seeking reimbursement.

3. Vague explanation of specific interventions and procedures performed
Too many times we find nothing more than "per protocol" to explain why a cardiac monitor was applied, an IV was initiated or some other procedure was performed. Just like the ambulance service must be medically necessary to be reimbursed by Medicare and other payers, the treatments provided must also be medically necessary.

Interventions and procedures should be performed in response to specific patient assessment findings, not simply because some protocol exists. Crew members must understand that the patient's PCR is part of that patient's medical record, not simply an internal document.

Crew members should not assume that those reading their PCR know their organization's protocols. The medical findings that suggest the need for each intervention, as well as the patient's response to that intervention, should be clearly documented.

4. No explanation for EMS-specific care and treatment
This is important with regard to two areas. First, is clearly explaining the transport itself and the service or care the patient required during the transport that could not be provided other than by trained medical professionals in an ambulance.

Second, in the case of a patient being transported from one facility to another, what specific services does the patient require that are not available at the facility of origin"

Simply stating, "Transported patient for higher level of care" tells the reader nothing. The PCR must make clear the care the patient required at the destination facility and why that care could not be provided in the facility of origin.

In addition, the PCR must show what professional medical care the patient required during transport to that facility.

5. Inadequate description of patient complaints or findings
The most common example of an inadequately described or quantified complaint or finding is with regard to a patient's pain. EMTs and paramedics should always describe a finding or complaint of pain by documenting completely the Onset, Provocation, Quality, Radiation, Severity and Time (OPQRST), as well as the patient's pain rating on a scale of zero to 10.

The word "pain" on a PCR is a trigger to remind the EMS provider to fully describe and document that pain.

Hemorrhage is another common finding that is inadequately described. Always describe the location and size of any wound, and quantify of blood loss.

Again, these are just two examples. Good PCR documentation will not just state conclusions or findings. It always describes and quantifies those findings accurately, honestly and objectively.

These five mistakes represent the most common documentation shortcomings we see on PCRs. Sadly, it is due to poor or incomplete documentation, and not an actual lack of medical necessity for an ambulance, that far too many patients are deprived of the coverage they deserve and too many ambulance services are deprived of fair compensation for the care they provide.



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Administration of resveratrol enhances cell-cycle arrest followed by apoptosis in DMBA-induced skin carcinogenesis in male Wistar rats

OBJECTIVE: Resveratrol (RES), present in fruits and plants, is a natural compound that has been shown various medicinal properties, including protection of cardiovascular disease and cancer risk. However, the effects of RES on skin cancer have not been investigated. The present work was designed to explore the anticancer potential of RES against chemical-induced skin carcinogenesis in rats.

MATERIALS AND METHODS: Skin carcinogenesis were induced in male Wistar rats by a single topical application of 7,12-dimethylbenz(a)anthracene (DMBA) and 2 weeks later, 12-O-tetradecanoylphorbol-13-acetate (TPA) were topically applied thrice a week to promote skin carcinogenesis. RES at a dose of 1 or 2 mg/kg body weight/week were administered to DMBA treated rats. The effects of RES on DMBA-modified cell-cycle arrest, apoptosis and protein expressions were analyzed by flow cytometry, immunohistochemistry and Western blot, respectively.

RESULTS: RES treatment caused a significant reduction of DMBA-induced tumor occurrence, tumor volume and tumor weight, as compared to DMBA control group. Further, RES treatment increases G2/M arrest and apoptosis by modulating cell-cycle and apoptosis regulated genes such as p53, p21, caspase-3, bax, survivin, cyclin-B and cdc-2 when compared with DMBA control group.

CONCLUSIONS: Taken together, the anticancer effect of RES is associated with regulation of cell-cycle and apoptosis in skin cancer, thereby attenuating skin cancer growth. Hence, these findings suggest that RES may be a therapeutic agent for skin cancer treatment.

L'articolo Administration of resveratrol enhances cell-cycle arrest followed by apoptosis in DMBA-induced skin carcinogenesis in male Wistar rats sembra essere il primo su European Review.



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Hydrochlorothiazide hypertension treatment induced metabolic effects in type 2 diabetes: a meta-analysis of parallel-design RCTs

OBJECTIVE: Thiazide diuretics are still widely used as an initial therapy in essential hypertension, sometimes in both hypertensive and diabetic patients. However, the metabolic effects in type 2 diabetes treated with a thiazide diuretic have not been fully elucidated.

MATERIALS AND METHODS: Randomized controlled trials (RCTs) were identified from the electronic databases: the Cochrane Library, MEDLINE, and PubMed web of knowledge. The trials compared the metabolic effects of hydrochlorothiazide (HCTZ) versus no- HCTZ hypertension treatment in type 2 diabetes.

RESULTS: A total of 368 papers showed a match, in the keyword search. Upon screening the title, reading the abstract and the entire article, 13 parallel-design RCTs, described in 7 reports, involving 720 patients, showed fasting glucose (FG) (SMD = 0.27, 95% CI 0.11-0.43) and HbA1c (SMD = 1.09, 95% CI 0.47-1.72)significantly increased in the patients treated with HCTZ groups and high-density lipoprotein-cholesterol (HDL-C) (SMD = -0.44, 95% CI -0.81- -0.08) decreased in the patients treated with low-dose HCTZ groups. Our study showed FG, HbA1c and HDL-C significantly affected in the patients treated with low-dose HCTZ groups.

CONCLUSIONS: Our study showed FG and HbA1c increased in the patients treated with the low-dose HCTZ groups, and HDL-C decreased in the patients. While thiazide diuretics are still a recommended medication of hypertension therapy for type 2 diabetes, treatment with low-dose HCTZ should be attempted to evaluate the effectiveness and adverse metabolic effects.

L'articolo Hydrochlorothiazide hypertension treatment induced metabolic effects in type 2 diabetes: a meta-analysis of parallel-design RCTs sembra essere il primo su European Review.



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D-mannose: a promising support for acute urinary tract infections in women. A pilot study

OBJECTIVE: Urinary tract infections still represent a significant bother for women and result in high costs to the health system. D-mannose is a simple sugar; it seems able to hinder bacteria adhesion to the urothelium. The present study aimed to determine whether D-mannose alone is effective in treating acute urinary tract infections in women and its possible utility in the management of recurrences.

PATIENTS AND METHODS: This is a pilot study, performed between April 2014 and July 2015 at Department of Gynaecological Obstetrics and Urologic Sciences of "Sapienza" University of Rome. A D-mannose compound was administered twice daily for 3 days and then once a day for 10 days. Changes in patients' symptoms, the therapeutic effects and changes in quality of life (QoL) were evaluated clinically and using a specifically validated questionnaire (UTISA). After described treatment, patients were randomized in receiving or not prophylaxis in the next 6 months.

RESULTS: Mean UTISA scores recorded after completing the treatment, compared with baseline scores, showed a significant improvement of the majority of symptoms (p < 0.05). D-mannose seemed to have had a significant positive effect on UTIs' resolution and QoL improvement (p = 0.0001). As prophylactic agent administered for 6 months, it showed promising results (4.5% vs. 33.3% recurrences in treated and untreated patients respectively).

CONCLUSIONS: The results of this study suggest that D-mannose can be an effective aid in acute cystitis management and also a successful prophylactic agent in a selected population; however, more studies will certainly be needed to confirm the results of our pilot study.

L'articolo D-mannose: a promising support for acute urinary tract infections in women. A pilot study sembra essere il primo su European Review.



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Role of vitamin D3 in regulation of interleukin-6 and osteopontin expression in liver of diabetic mice

OBJECTIVE: To study the link between hepatic interleukin-6 (IL-6) and osteopontin (OPN) gene expression and vitamin D3 status associated with type 1 diabetes in mice; and to evaluate the effects of vitamin D3 treatment (800 IU/kg of body weight for 6 weeks) on diabetes-induced impairments.

MATERIALS AND METHODS: mRNA levels of IL-6 and OPN were measured by quantitative RT-PCR. Blood serum 25OHD3 was assayed by ELISA.

RESULTS: It was shown that induction of IL-6 in diabetic liver is accompanied by increased expression of OPN. Changes in OPN and IL-6 RNA levels correlated with a lack of 25OHD3 in serum. Vitamin D3 treatment restored 25OHD3 that led to a substantial reduction of OPN and IL-6 mRNA levels.

CONCLUSIONS: Diabetes-induced vitamin D3 deficiency was associated with increased hepatic levels of IL-6 and OPN mRNA and these changes were countered by vitamin D3 administration.

L'articolo Role of vitamin D3 in regulation of interleukin-6 and osteopontin expression in liver of diabetic mice sembra essere il primo su European Review.



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Autologous hematopoietic stem cell transplantation in patients with end-stage liver disease: a 5-year follow-up study of 48 patients

OBJECTIVE: To investigate the long-term therapeutic effect of autologous hematopoietic stem cell transplantation in patients with End-stage Liver Disease (ESLD).

PATIENTS AND METHODS: Forty-eight ESLD patients underwent autologous CD34+ stem cell transplantation were retrospectively reviewed. Changes in clinical and biochemical data, complications, and quality of life were monitored at 3, 6, 12, 36, and 60 months following the stem cell transplantation. Liver biopsies were obtained for histopathological analysis using Ishak system.

RESULTS: Marked improvement in clinical and biochemical data was observed during the long-term follow-up. Serum albumin was significantly increased (p<0.001), while total serum bilirubin, prothrombin time (PT), and international normalized ratio (INR) were all significantly decreased (p<0.001). Ishak inflammation and fibrosis scores were significantly decreased with the increased time (p<0.001). The number of patients with ascites, model of end-stage liver disease (MELD) score, Child-Pugh class, and indocyanine green (ICG) score were all markedly reduced with increased time. Meanwhile, the quality of life score of the patients was significantly increased (p<0.001). Six patients died during the 5-years follow-up, and complications occurred in 17 patients. The incidence of complications was significantly associated with mortality of the patients (p<0.05).

CONCLUSIONS: The study provided the evidence that autologous CD34+ stem cell transplantation could offer a long-term therapeutic benefit to patients with ESLD. The complications occurred during the process was significantly associated with survival of the patients. Future studies on a large cohort of patients are needed to confirm the long-term effect of stem cell therapy on ESLD.

L'articolo Autologous hematopoietic stem cell transplantation in patients with end-stage liver disease: a 5-year follow-up study of 48 patients sembra essere il primo su European Review.



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Bone marrow cells differentiation into organ cells using stem cell therapy

Bone marrow cells (BMC) are progenitors of bone, cartilage, skeletal tissue, the hematopoiesis-supporting stroma and adipocyte cells. BMCs have the potential to differentiate into neural cells, cardiac myocytes, liver hepatocytes, chondrocytes, renal, corneal, blood, and myogenic cells. The bone marrow cell cultures from stromal and mesenchymal cells are called multipotent adult progenitor cells (MAPCs). MAPCs can differentiate into mesenchymal cells, visceral mesoderm, neuroectoderm and endoderm in vitro. It has been shown that the stem cells derived from bone marrow cells (BMCs) can regenerate cardiac myocytes after myocardial infarction (MI). Adult bone marrow mesenchymal stem cells have the ability to regenerate neural cells. Neural stem/progenitor cells (NS/PC) are ideal for treating central nervous system (CNS) diseases, such as Alzheimer's, Parkinson's and Huntington disease. However, there are important ethical issues about the therapeutic use of stem cells. Neurons, cardiac myocytes, hepatocytes, renal cells, blood cells, chondrocytes and adipocytes regeneration from BMCs are very important in disease control. It is known that limbal epithelial stem cells in the cornea can repair the eye sight and remove symptoms of blindness. Stem cell therapy (SCT) is progressing well in animal models, but the use of SCT in human remains to be explored further.

L'articolo Bone marrow cells differentiation into organ cells using stem cell therapy sembra essere il primo su European Review.



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A cost effective parameter for predicting the troponin elevation in patients with carbon monoxide poisoning: red cell distribution width

OBJECTIVE: Carbon monoxide (CO) poisoning is very common worldwide. Despite the fact that CO is known to have cardiotoxic effects, as it has non-specific symptoms; cardiotoxicity could easily be overlooked, especially when troponin is not measured. The present study aimed to evaluate the association between troponin I levels and red cell distribution width (RDW) levels, which can be measured rapidly, easily, and affordably in the Emergency Room (ER).

PATIENTS AND METHODS: This single-center observational study included a total of 504 consecutive patients, who presented to the ER due to CO poisoning between January 2011 and June 2015. The diagnosis of CO poisoning was made according to the medical history and carboxyhemoglobin (COHb) level of >5%. Elevated troponin test levels, which measure >0.04 ng/ml for our laboratory, were accepted as positive.

RESULTS: Patients (mean age 37±14) were classified into two groups: those who had positive troponin levels (38%) and those that did not. Patients with positive troponin, who were older, had longer CO exposure time and higher creatinine, COHb and RDW levels at the index admission following CO poisoning than patients with negative troponin. In a multivariate logistic regression model with forward stepwise method, age, COHb level, CO exposure time, and RDW (HR=1.681, 95% CI: 1.472-1.934, p<0.001) remained associated with an increased risk of troponin positivity following adjustment for the variables that were statistically significant in the univariate analysis and correlated with RDW.

CONCLUSIONS: In patients presenting to the ER with CO poisoning, RDW can be helpful for the risk stratification of troponin positivity.

L'articolo A cost effective parameter for predicting the troponin elevation in patients with carbon monoxide poisoning: red cell distribution width sembra essere il primo su European Review.



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Amniotic fluid stem cells: an ideal resource for therapeutic application in bone tissue engineering

OBJECTIVE: Skeletal diseases, both degenerative and secondary to trauma, infections or tumors, represent an ideal target for regenerative medicine and in the last years, stem cells have been considered as good candidates for in vitro and in vivo bone regeneration. To date, several stem cell sources, such as adult mesenchymal stem cells, embryonic stem cells (ESCs) and induced pluripotent stem cells (iPSCs), have shown significant osteogenic potential.

MATERIALS AND METHODS: In this narrative review, we analyze the possible advantages of the use of AFSCs in the treatment of skeletal diseases, especially through the application of tissue engineering and biomaterials.

RESULTS: Among the different sources of stem cells, great attention has been recently devoted to amniotic fluid-derived stem cells (AFSC) characterized by high renewal capacity and ability to differentiate along several different lineages.

CONCLUSIONS: Due to these features, AFSCs represent an interesting model for regenerative medicine, also considering their low immunogenicity and the absence of tumor formation after transplantation in nude mice.

L'articolo Amniotic fluid stem cells: an ideal resource for therapeutic application in bone tissue engineering sembra essere il primo su European Review.



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Expression of microRNA miR-34a inhibits leukemia stem cells and its metastasis

OBJECTIVE: To study the expression profile and role of microRNA miR-34a in Leukemia stem cells (LSC) and during its metastasis. We examined upon the enforced expression of miR-34a in TIM3 positive leukemia stem cells and study the impact of leukemia stem cells and its metastasis.

PATIENTS AND METHODS: Samples were collected from acute myeloid leukemia (AML) children along with controls. Immunohistochemistry analysis was performed with TIM3 antibody followed by Sorting of TIM3 positive cells by flow cytometry and the expression level of miR-34a was quantified by qRT-PCR assay.

RESULTS: Immunohistochemistry in accordance with Sorting of TIM3 positive cells by flow cytometry concludes that the leukemia stem cells (LSC) were present in the samples. Induced expression of miR-34a in TIM3 positive leukemia stem cells (LSC), inhibits the clonogenic expansion, tumor progression and metastasis of leukemia.

CONCLUSIONS: The enforced expression of miR-34a in TIM3 positive leukemia stem cells inhibits the leukemia stem cells and its metastasis. Our study illustrates that miR-34a is a key regulator and which will be developed as a novel therapeutic agent against leukemia stem cells (LSC).

L'articolo Expression of microRNA miR-34a inhibits leukemia stem cells and its metastasis sembra essere il primo su European Review.



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Transarterial chemoembolization with degradable starch microspheres (DSM-TACE): an alternative option for advanced HCC patients? Preliminary results

OBJECTIVE: To assess safety, feasibility and effectiveness of transarterial chemoembolization with degradable-starch-microspheres (DSM-TACE) in the treatment of patients with advanced hepatocellular carcinoma (HCC) dismissing or ineligible for multikinase-inhibitor chemotherapy administration (Sorafenib) due to unbearable side effects or clinical contraindications.

PATIENTS AND METHODS: Six consecutive advanced HCC patients dismissing Sorafenib because of unbearable side effects or worsened clinical conditions were enrolled in our prospective single-center pilot study. DSM-TACE was performed via a lobar approach, based on extent and distribution of the disease (1 treatment session for every lobe involved, with a 2-week interval in case of bilobar disease). Tumor response based on mRECIST criteria was evaluated on MD-CT performed at 1 month after "complete treatment" and every 3 months thereafter.

RESULTS: Eleven treatments were performed, and technical success was achieved in all patients. No intra/peri-procedural death/major complications occurred. No signs of liver failure or systemic toxicity were detected. At one month follow-up, 5 partial responses (83.3%) and 1 progression disease (16.6%) with an overall disease control (ODC) of 83.3% were observed. In two patients with ODC and residual viable tumor higher than 50%, a repeated DSM-TACE treatment was performed. During the mean follow-up of 11 months (range: 4-14 months), an ODC of 66.6% was obtained. Progression-free survival was 5.5 months with a cumulative 6-month and 1-year overall survival rates of 83.3% and 66.6%, respectively.

CONCLUSIONS: DSM-TACE seems to be a promising option for advanced HCC patients ineligible for Sorafenib administration or dismissing it due to progressive disease or unbearable side effects.

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Rare complication of pediatric inguinal hernia repair: case report of transection of the femoral vein

Abstract

Introduction

The incidence of pediatric inguinal hernias ranges up to 13 %. Currently, many different techniques are being used to successfully perform herniotomy and risks of complications are minor.

Case report

This case report describes a 4-year-old male patient who underwent routine herniotomy during which the femoral vein was mistaken for hernial sac and thereby the femoral vein was transected. Postoperative vascular duplex ultrasonography revealed a complete transection of the right common femoral vein. Multiple treatment options such as venous reconstruction and interposition were considered, but a conservative treatment was chosen. At 6 months postoperative, the patient only shows minor complaints. Long-term results remain uncertain, especially since no similar case has previously been described in the current literature.



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Adsorption Behavior and Removal Mechanism of Arsenic from Water by Fe(III)-Modified 13X Molecular Sieves

Abstract

Presented are the synthesis and characterization of Fe(III)-modified 13X molecular sieves and their application as a novel adsorbent for removing arsenic from aqueous solutions. Batch experimental results showed that Fe(III) adsorption by 13X molecular sieves matched well with the Langmuir adsorption isotherm. The adsorption kinetics of arsenic on the Fe(III)-modified molecular sieves fit well with a pseudo-second-order model. The Langmuir adsorption isotherms of arsenic adsorption indicated the highest adsorption capacities of 1167.79 for As(V) at pH 4 and 731.56 mg/kg for As(III) at pH 9. The Fe(III)-modified 13X molecular sieves removed much more As(V) than As(III) at equivalent arsenic concentrations, regardless of the pH conditions. After As(V) removal, the Fe(III)-modified 13X molecular sieves were characterized by PXRD, SEM-EDX, and ATR-FTIR to analyze the morphology and arsenic speciation. The results of PXRD and SEM-EDX spectroscopy indicated that the material was physically stable after As(V) adsorption. ATR-FTIR spectroscopy showed that the formation of inner-sphere surface complexations between Fe hydroxide on the surface of the molecular sieves and As(V) could be a plausible mechanism for the uptake of arsenic by the Fe(III)-modified 13X molecular sieves. Therefore, the relatively low cost and remarkable arsenic-adsorption performance make the title material a promising absorbent for the treatment of arsenic in wastewater.

Graphical Abstract

Arsenic removal by Fe(III)-modified 13X molecular sieves


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Toward interfacing organic semiconductors with ferromagnetic transition metal substrates: enhanced stability via carboxylate anchoring

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Chem. Commun., 2016, Accepted Manuscript
DOI: 10.1039/C6CC05009C, Communication
Runyuan Han, Florian Blobner, Johannes Bauer, David Andrew Duncan, Johannes Barth, Peter Feulner, Francesco Allegretti
We demonstrate that chemically well-defined aromatic self-assembled monolayers (SAMs) bonded via a carboxylate head group to ferromagnetic (FM = Co, Ni, Fe) transition metal surfaces can be prepared at ambient...
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Halogen Bonded Supramolecular Capsules: A Challenging Test Case for Quantum Chemical Methods

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Chem. Commun., 2016, Accepted Manuscript
DOI: 10.1039/C6CC03664C, Communication
Rebecca Sure, Stefan Grimme
Recently, Diederich et al. synthesized the first supramolecular capsule with a well-defined four-point halogen bonding interaction [Diederich et al., Angew. Chem, 2015, 54, 12339]. This interesting system represents a challenging...
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Selective Parathyroid Hormone Venous Sampling in Patients with Persistent or Recurrent Primary Hyperparathyroidism and Negative, Equivocal or Discordant Noninvasive Imaging

Abstract

Background

In patients with persistent (P-PHPT) or recurrent (R-PHPT) primary hyperparathyroidism, preoperative localization is important. Selective parathyroid hormone venous sampling (sPVS) is an invasive technique that can be used to regionalize and/or lateralize the source of PHPT when noninvasive imaging studies are nonlocalizing. The aim of the present study was to assess the role of sPVS in the preoperative evaluation of patients with P-PHPT or R-PHPT and negative, equivocal, or discordant noninvasive imaging localization.

Methods

After IRB-approval a retrospective review of all patients with P-PHPT or R-PHPT and nonlocalizing noninvasive imaging that underwent sPVS from 2000 to 2014 was performed. The location of the source of PHPT at sPVS was predicted by a parathyroid hormone (PTH) gradient and compared to the surgical, pathology, and biochemical follow-up data as the gold standard. Sensitivity and positive predictive value (PPV) were calculated.

Results

Of 30 patients who underwent sPVS, 12 patients did not undergo surgical exploration due to negative or non-localizing PTH gradient (n = 8) or opted for medical management (n = 4). Of the 18 patients who underwent surgical exploration, 17 (94 %) had a positive PTH gradient and pathologic parathyroid tissue identified at surgery. Sensitivity and PPV of sPVS were 93 and 77 %, respectively, for all surgical cases, 86 and 60.0 % for cervical cases (n = 11), and 100 and 100 % for mediastinal cases (n = 7). Sixteen patients (89 %) were surgically cured.

Conclusions

In patients with P-PHPT or R-PHPT and nonlocalizing imaging studies, sPVS is a sensitive test for localizing the source of PHPT when a positive PTH gradient is present.



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Catching Z's

We have all had those mornings and nights: the ones where you wake up feeling groggy and completely unrested, or where you toss and turn relentlessly. Have you ever wondered if there were things you could do to limit these feelings? Our experts have some tips and guidelines on getting your most restful sleep and waking up rejuvenated.



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