Publication date: Available online 1 September 2017
Source:Allergology International
Author(s): Christina E. West, Majda Dzidic, Susan L. Prescott, Maria C. Jenmalm
Large-scale biodiversity loss and complex changes in social behaviors are altering human microbial ecology. This is increasingly implicated in the global rise in inflammatory diseases, most notably the "allergy epidemic" in very early life. Colonization of human ecological niches, particularly the gastrointestinal tract, is critical for normal local and systemic immune development and regulation. Disturbances in composition, diversity and timing of microbial colonization have been associated with increased allergy risk, indicating the importance of strategies to restore a dysbiotic gut microbiota in the primary prevention of allergic diseases, including the administration of probiotics, prebiotics and synbiotics. Here, we summarize and discuss findings of randomized clinical trials that have examined the effects of these microbiome-related strategies on short and long-term allergy preventative effects – including new guidelines from the World Allergy Organization which now recommend probiotics and prebiotics for allergy prevention under certain conditions. The relatively low quality evidence, limited comparative studies and large heterogeneity between studies, have collectively hampered recommendations on specific probiotic strains, specific timing and specific conditions for the most effective preventive management. At the same time the risk of using available products is low. While further research is needed before specific practice guidelines on supplement probiotics and prebiotics, it is equally important that the underlying dietary and lifestyle factors of dysbiosis are addressed at both the individual and societal levels.
http://ift.tt/2vSPYVo
Medicine by Alexandros G.Sfakianakis,Anapafseos 5 Agios Nikolaos,Crete 72100,Greece,tel :00302841026182 & 00306932607174
Παρασκευή 1 Σεπτεμβρίου 2017
Bugging allergy; role of pre-, pro- and synbiotics in allergy prevention
Development of a questionnaire to evaluate asthma control in Japanese asthma patients
Publication date: Available online 1 September 2017
Source:Allergology International
Author(s): Yuji Tohda, Soichiro Hozawa, Hiroshi Tanaka
BackgroundThe asthma control questionnaires used in Japan are Japanese translations of those developed outside Japan, and have some limitations; a questionnaire designed to optimally evaluate asthma control levels for Japanese may be necessary. The present study was conducted to validate the Japan Asthma Control Survey (JACS) questionnaire in Japanese asthma patients.MethodsA total of 226 adult patients with mild to severe persistent asthma were enrolled and responded to the JACS questionnaire, asthma control questionnaire (ACQ), and Mini asthma quality of life questionnaire (Mini AQLQ) at Weeks 0 and 4. The reliability, validity, and sensitivity/responsiveness of the JACS questionnaire were evaluated.ResultsThe intra-class correlation coefficients (ICCs) were within the range of 0.55–0.75 for all JACS scores, indicating moderate/substantial reproducibility. For internal consistency, Cronbach's alpha coefficients ranged from 0.76 to 0.92 in total and subscale scores, which were greater than the lower limit of internal consistency. As for factor validity, the cumulative contribution ratio of four main factors was 0.66. For criterion-related validity, the correlation coefficients between the JACS total score and ACQ5, ACQ6, and Mini AQLQ scores were −0.78, −0.78, and 0.77, respectively, showing a significant correlation (p < 0.0001).ConclusionsThe JACS questionnaire was validated in terms of reliability and validity. It will be necessary to evaluate the therapeutic efficacy measured by the JACS questionnaire and calculate cutoff values for the asthma control status in a higher number of patients.Clinical Trial registrationUMIN000016589
http://ift.tt/2wrNT6x
Venous thromboembolism and hyperhomocysteinemia as first manifestation of pernicious anemia: a case series
Hyperhomocysteinemia has been suspected of favoring thrombosis. Several case–control studies and even a meta-analysis have confirmed a link between venous thrombosis and hyperhomocysteinemia. Homocysteine is d...
http://ift.tt/2erEpy7
Cetuximab with radiotherapy as an alternative treatment for advanced squamous cell carcinoma of the temporal bone
Source:Auris Nasus Larynx
Author(s): Koji Ebisumoto, Kenji Okami, Masashi Hamada, Daisuke Maki, Akihiro Sakai, Kosuke Saito, Fukuko Shimizu, Shoji Kaneda, Masahiro Iida
The prognosis of advanced temporal bone cancer is poor, because complete surgical resection is difficult to achieve. Chemoradiotherapy is one of the available curative treatment options; however, its systemic effects on the patient restrict the use of this treatment. A 69-year-old female (who needed peritoneal dialysis) presented at our clinic with T4 left external auditory canal cancer and was treated with cetuximab plus radiotherapy (RT). The primary lesion showed complete response. The patient is currently alive with no evidence of disease two years after completion of the treatment and does not show any late toxicity. This is the first advanced temporal bone cancer patient treated with RT plus cetuximab. Cetuximab plus RT might be a treatment alternative for patients with advanced temporal bone cancer.
http://ift.tt/2ewpHcK
Nasal interleukin 25 as a novel biomarker for patients with chronic rhinosinusitis with nasal polyps and airway hypersensitiveness
Chronic rhinosinusitis with nasal polyps (CRSwNP) is a chronic inflammatory disease of the upper airway and is tightly linked with airway hyperresponsiveness (AHR) and asthma. However, the surrogate biomarkers for indicating AHR and asthma in patients with CRSwNP remain elusive.
http://ift.tt/2er1gtJ
Assessing hypoxia risk during air travel after a severe asthma exacerbation in children
Decreased atmospheric cabin pressure in commercial aircrafts (cruising altitude 9,000–13,000 m), requiring cabin pressurization from 1,530 to 2,440 m, is equivalent to breathing a fraction of inspired oxygen (FiO2) of 15.1% at sea level.
http://ift.tt/2iPzVq1
Efectividad del colgajo de músculo pectoral mayor miofascial en la reducción de fístulas salivares tras laringectomía total de rescate
Source:Acta Otorrinolaringológica Española
Author(s): Carlos Miguel Chiesa Estomba, Jose Angel González García, Jon Alexander Sistiaga Suarez, Izaskun Thomas Arrizabalaga, Ekhiñe Larruscain Sarasola, Xabier Altuna Mariezcurrena
IntroducciónLa fístula faringocutánea es la más frecuente de las complicaciones poslaringectomía total. Su incidencia varía entre un 9-25% en pacientes poslaringectomía total primaria, hasta un 14-57% en laringectomía de rescate posradioterapia o posquimioterapia+radioterapia. El colgajo de músculo pectoral mayor miofascial (PMMF) se postula como una herramienta útil para disminuir la incidencia de esta complicación.Material y métodoAnálisis retrospectivo de pacientes tratados mediante laringectomía total de rescate, asociada o no a refuerzo de sutura faríngea con colgajo de PMMF.ResultadosVeinte pacientes fueron incluidos, 18 hombres (90%) y 2 mujeres (10%), en 10 de los cuales se utilizó colgajo de PMMF. La edad promedio fue de 66,65 años. Diecisiete (85%) presentaron un tumor laríngeo y 3 (15%) un tumor de hipofaringe. Ocho (80%) pacientes del grupo sin PMMF presentaron fístula en el postoperatorio, mientras que tan solo 2 (20%) pacientes del grupo de PMMF presentaron fístula durante el postoperatorio (p=0,005). El tiempo promedio para el cierre de la fístula fue significativamente menor en los casos en que se empleó el PMMF (16±11 vs. 76,8±67 días, p=0,001), así como la estancia hospitalaria (19,6±18 vs. 83,9±77 días, p0,001).ConclusiónEl uso del PMMF se asocia a una menor tasa de fístulas poslaringectomía total de rescate en pacientes tratados de forma primaria mediante protocolo de conservación de órgano por cáncer de laringe/hipofaringe, y favorece la cicatrización local disminuyendo el tiempo promedio de cierre de fístulas y la estancia media hospitalaria.IntroductionPharyngocutaneous fistula is the most frequent complication after total laryngectomy. Its incidence varies between 9%-25% in post primary total laryngectomy patients, to 14%-57% in salvage laryngectomy post radiotherapy or post chemotherapy + radiotherapy. The pectoralis major myofascial flap (PMMF)is postulated as a useful tool to decrease the incidence of this complication.Materials and methodRetrospective analysis of a group of patients treated by salvage laryngectomy, associated or not with pharyngeal closure reinforcement with PMMF.ResultsTwenty patients were included, 18 males (90%) and 2 females (10%), in 10 of whom the PMMF was used. The average age was 66.65 years. Seventeen (85%) had a laryngeal tumour and 3 (15%) had a hypopharyngeal tumour. Eight (80%) patients in the non-PMMF group had postoperative fistula, whereas only 2 (20%) patients in the PMMF group had a fistula during the postoperative period (P=.005). The mean time for fistula closure was significantly shorter in the cases where PMMF flap was used (16±11 days vs. 76.8±67 days, P=.001), as was hospital stay (19. 6±18 days vs. 83.9±77 days, P=.001).ConclusionThe use of PMMF in our series is associated with a lower rate of post salvage laryngectomy fistulas in patients treated primarily by organ preservation protocol for laryngeal/hypopharyngeal cancer. In turn, it promotes local healing by decreasing the mean duration of fistula closure and the mean hospital stay.
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oVEMP as an objective indicator of successful repositioning maneuver
Publication date: Available online 31 August 2017
Source:Acta Otorrinolaringológica Española
Author(s): Samir Asal, Osama Sobhy, Amany Balbaa
Background and objectiveBenign paroxysmal positioning vertigo (BPPV) is the most common peripheral vestibular disorder. Canalolithiasis in the posterior semi-circular canal is the most common underlying pathology that can be treated effectively by repositioning maneuvers. Our hypothesis suggested that successful maneuvers can lead to repositioning of dislodged otoconia to the utricle.Materials and methodsAir conducted oVEMP, which is thought to originate from the contra-lateral utricular organ was measured in twenty patients with unilateral BPPV and we compared n1–p1 peak to peak amplitude of the affected ears in 3 separate intervals: on pre-treatment when typical nystagmus was confirmed, immediately after, and 1 week after repositioning maneuvers to assess change, if any, in amplitude.ResultsThis study showed significant increase of oVEMP amplitude in the affected ears after successful repositioning maneuver that was more significant after 1 week.ConclusionsoVEMP can be used as a reliable objective test for ensuring a successful maneuver rather than subjective dependence on the patient's symptoms, which may be misleading due to a remission.
http://ift.tt/2wYVz1e
Análisis cepstral de la voz normal y patológica en adultos españoles. Medida de la prominencia del pico cepstral suavizado en vocales sostenidas versus habla conectada
Publication date: Available online 1 September 2017
Source:Acta Otorrinolaringológica Española
Author(s): Jonathan Delgado-Hernández, Nieves M. León-Gómez, Laura M. Izquierdo-Arteaga, Yanira Llanos-Fumero
Introducción y objetivosEn los últimos años se ha incrementado el uso de medidas cepstrales para la evaluación acústica de la voz. Uno de los parámetros más investigados es la prominencia del pico cepstral suavizado (CPPs). Los objetivos de este trabajo son conocer la utilidad de esta medida acústica en la evaluación objetiva de las alteraciones de la voz en español y determinar qué tipo de muestra de voz (vocales sostenidas o habla conectada) es la más sensible para evaluar la severidad de la disfonía.MétodoEn este estudio participaron 40 sujetos, 20 normofónicos y 20 con disfonía. Se grabaron 2 muestras de voz para cada sujeto (una/a/sostenida y 4 frases fonéticamente balanceadas) y se calculó la CPPs con el programa Praat. Tres expertos valoraron perceptivamente la voz de la muestra con el parámetro grado de la escala GRABS.ResultadosSe encontraron valores significativamente menores en las voces disfónicas, tanto para la/a/(t[38]=4,85, p<0,000) como para las frases (t[38]=5,75, p<0,000). En relación con el tipo de muestra de voz más indicado para evaluar la severidad de las alteraciones de la voz se encontró una fuerte correlación con la escala acústico-perceptiva de la CPPs calculada a partir del habla conectada (rs = –0,73) y moderada con la calculada a partir de la vocal sostenida (rs=–0,56).ConclusiónLos resultados de este estudio preliminar apuntan a que la CPPs es una buena medida para detectar la disfonía y para evaluar objetivamente el grado de severidad de las alteraciones en la voz.Introduction and objectivesIn recent years, the use of cepstral measures for acoustic evaluation of voice has increased. One of the most investigated parameters is smoothed cepstral peak prominence (CPPs). The objectives of this paper are to establish the usefulness of this acoustic measure in the objective evaluation of alterations of the voice in Spanish and to determine what type of voice sample (sustained vowels or connected speech) is the most sensitive in evaluating the severity of dysphonia.MethodForty subjects participated in this study 40, 20 controls and 20 with dysphonia. Two voice samples were recorded for each subject (one sustained vowel/a/and four phonetically balanced sentences) and the CPPs was calculated using the Praat programme. Three raters perceptually evaluated the voice sample with the Grade parameter of GRABS scale.ResultsSignificantly lower values were found in the dysphonic voices, both for/a/(t[38]= 4.85, P<.000) and for phrases (t[38] = 5,75, P<.000). In relation to the type of voice sample most suitable for evaluating the severity of voice alterations, a strong correlation was found with the acoustic-perceptual scale of CPPs calculated from connected speech (rs = –0.73) and moderate correlation with that calculated from the sustained vowel (rs = –0,56).ConclusionThe results of this preliminary study suggest that CPPs is a good measure to detect dysphonia and to objectively assess the severity of alterations in the voice.
http://ift.tt/2xEpARo
A prospective analysis evaluating tissue biopsy location and its clinical relevance in chronic rhinosinusitis with nasal polyps
Background
Chronic rhinosinusitis with nasal polyps (CRSwNP) has a high propensity for recurrence. Studies suggest that eosinophilia influences disease severity and surgical outcomes, but the selection of sinonasal site for measuring eosinophilia has not been examined. The aim of this study was to investigate how region-specific tissue eosinophilia affects radiographic severity, comorbidity prevalence, and polyp recurrence risk following sinus surgery.
Methods
Eosinophil cationic protein (ECP) levels in uncinate tissue (UT) and nasal polyp (NP) homogenates from 116 CRSwNP patients were measured using enzyme-linked immunosorbent assay (ELISA). Clinical history, radiographic severity, and time to polyp recurrence were obtained from electronic health records. The correlations between baseline Lund-Mackay scores and comorbidities were compared between UT and NP ECP levels. Cox regression and Kaplan-Meier analysis were then performed to assess whether UT or NP ECP better predicted recurrence. Censoring occurred at 4 years or at last follow-up if there was no endoscopic diagnosis of recurrent polyps.
Results
Lund-Mackay scores were significantly correlated with UT and NP ECP (r = 0.46 and 0.26 respectively, p < 0.05). UT but not NP ECP was significantly higher in patients with asthma (p < 0.01) and aspirin-exacerbated respiratory disease (AERD) (p < 0.05). Polyp recurrence risk was only significantly higher for patients with eosinophilic UT tissue (hazard ratio [HR] = 2.84, p = 0.025). When measured in NP, eosinophilia did not predict recurrence.
Conclusion
Although ECP in NP was higher than in UT tissue, eosinophilia in UT tissue was a more clinically coherent biomarker of baseline radiographic severity, comorbid asthma and AERD, and prospective polyp recurrence risk than NP eosinophilia.
http://ift.tt/2guK76K
Therapeutic potential of α-lipoic acid derivative, sodium zinc histidine dithiooctanamide, in a mouse model of allergic rhinitis
Background
Oxidative stress is involved in various diseases, including allergies. Several studies have pointed to the preventive and therapeutic potential of antioxidants in allergic disorders. However, little is known about the immunomodulatory effects of antioxidants in type I hypersensitivity. In this study we aimed to explore the impact of a water-soluble antioxidant and α-lipoic acid derivative, sodium zinc histidine dithiooctanamide (DHL-HisZn), on mast-cell- and T-cell-mediated allergic and immune responses both in vitro and in vivo.
Methods
The therapeutic impact of DHL-HisZn on mast-cell-mediated type I hypersensitivity was evaluated by a mast-cell degranulation assay using bone marrow-derived mast cells and by a mouse model of ovalbumin (OVA)-induced allergic rhinitis. The effect of DHL-HisZn on the proportion of regulatory T cells (Tregs) was evaluated using flow cytometry.
Results
During the course of OVA-induced allergic rhinitis in mice, serum nitrate was elevated, suggesting the involvement of oxidative stress in allergic responses. DHL-HisZn not only suppressed mast-cell degranulation but also ameliorated OVA-induced nasal hypersensitivity, with significant suppression of serum nitrate. DHL-HisZn treatment significantly suppressed OVA-specific immunoglobulin E (IgE) but enhanced OVA-specific IgG2a in OVA-sensitized and nasal-challenged mice. Furthermore, DHL-HisZn treatment suppressed interleukin-17 production in OVA-stimulated splenocytes. Finally, we demonstrated the induction of Tregs by DHL-HisZn in concanavalin A blasts.
Conclusions
These findings suggest that DHL-HisZn may regulate mast-cell-, T-helper 2 (Th2)-, and Th17-mediated allergic and immune responses by induction of Tregs.
http://ift.tt/2eLUXl2
Unique heterozygous presentation in an infant with DNA ligase IV syndrome
Source:Annals of Allergy, Asthma & Immunology
Author(s): Barbara A. Brunet, Nina Dave
http://ift.tt/2wpKl4A
Parental perception of anxiety in children with eosinophilic esophagitis in a tertiary care center
Source:Annals of Allergy, Asthma & Immunology
Author(s): Jaison Jose, Amy Virojanapa, Erik B. Lehman, Alexandra Horwitz, Tracy Fausnight, Punit Jhaveri, Chandran Alexander, Pooja Jhaveri
http://ift.tt/2vQC9a7
Management around invasive procedures in mastocytosis
Source:Annals of Allergy, Asthma & Immunology
Author(s): Maud A.W. Hermans, Nicolette J.T. Arends, Roy Gerth van Wijk, P. Martin van Hagen, Hanneke C. Kluin-Nelemans, Hanneke N.G. Oude Elberink, Suzanne G.M.A. Pasmans, Paul L.A. van Daele
ObjectiveMastocytosis is a chronic hematologic disorder that is characterized by the accumulation of aberrant mast cells and typically involves the skin and/or bone marrow. Patients with mastocytosis are at increased risk of anaphylaxis. Based on theoretical assumptions, medical procedures requiring general anesthesia or radiocontrast media are deemed hazardous for patients with mastocytosis. The objective of this article is to provide a comprehensive overview of the actual risk of iatrogenic anaphylaxis and provide recommendations for daily practice.Data SourcesVarious scientific search engines were used (eg, PubMed and Medline).Study SelectionsBecause of the paucity of high-level studies on this topic, all available evidence was considered, including case reports.ResultsReliable data on the incidence of iatrogenic anaphylaxis in mastocytosis are lacking. However, although the incidence as reported in (retrospective) cohort studies is higher than in the general population, it is still lower than commonly anticipated, with an incidence of 5.4% in 1 study. Adequate premedication and avoidance of certain physical stimuli can further decrease this risk by 10-fold. The role of drugs as elicitors of anaphylaxis is perhaps overestimated, and physical stimuli are at least as important in inducing release of mast cell mediators.ConclusionThis article provides practical recommendations for the management of invasive procedures in patients with mastocytosis based on current knowledge of this topic.
http://ift.tt/2wpNdym
Eosinophil-derived neurotoxin as a biomarker for disease severity and relapse in recalcitrant atopic dermatitis
Publication date: Available online 31 August 2017
Source:Annals of Allergy, Asthma & Immunology
Author(s): Hwan Soo Kim, Ji Hye Kim, Yu Mi Seo, Yoon Hong Chun, Jong-seo Yoon, Hyun Hee Kim, Joon Sung Lee, Jin Tack Kim
BackgroundEosinophils are encountered in many skin diseases, but the role of eosinophils in atopic dermatitis (AD) remains uncertain.ObjectiveTo examine the role of serum eosinophil-derived neurotoxin (EDN), eosinophil cationic protein (ECP), and total IgE as a biomarker of disease severity and relapse in severe recalcitrant AD.MethodsWe enrolled 99 patients with AD: 37 with severe recalcitrant AD, 20 with severe AD, and 42 with mild to moderate AD. We examined the difference in serum level of total IgE, ECP, and EDN between the groups and whether any correlation existed between disease severity and ECP or EDN. Lastly, difference in levels of ECP or EDN between those who experienced relapse was examined in the severe recalcitrant group.ResultsSerum levels of total IgE, ECP, and EDN were significantly higher in the severe recalcitrant AD group and severe AD group compared with the mild to moderate AD group. No significant difference was found in serum levels of total IgE, ECP, and EDN between the severe recalcitrant group and severe group. EDN had a significant positive correlation with the SCORing Atopic Dermatitis. No significant correlation was found between EDN and ECP. In the severe recalcitrant group, 29.7% of patients experienced relapse, and EDN was significantly higher in those who experienced relapse. The cutoff value of EDN for predicting relapse was 64.5.ConclusionEDN correlated with the disease severity of AD. EDN may predict relapse in severe recalcitrant AD. The EDN serum level could be considered a candidate molecule as a clinical biomarker for evaluating AD disease activity and a predictor of relapse.
http://ift.tt/2wpHQPO
Evaluation of periprocedural hypersensitivity reactions
Publication date: Available online 31 August 2017
Source:Annals of Allergy, Asthma & Immunology
Author(s): Melissa Iammatteo, Taha Keskin, Elina Jerschow
BackgroundIdentifying the cause of periprocedural hypersensitivity reactions (HSRs) remains challenging because of the multitude of medications involved. Antibiotics are the most common cause in the United States, whereas neuromuscular blocking agents are most common in Europe.ObjectiveTo identify causative agents for periprocedural HSRs.MethodsThis study was a 7-year retrospective medical record review of patients evaluated between December 2009 and January 2017 at a drug allergy center in Bronx, New York for periprocedural HSRs, defined as occurring soon before, during, or soon after a medical procedure or operation with or without general anesthesia. Demographics, description of historical HSRs, results of testing to potential causative medications, and tolerance of subsequent anesthesia were reviewed.ResultsThirty-four patients completed a comprehensive evaluation. Skin testing identified an IgE-mediated cause in 22 patients (64.7%). The most common causative class of medications was induction agents (n = 9 [36%]), with midazolam being the most frequently implicated (n = 6 [3 positive skin test results, 3 equivocal skin test results]). Cefazolin was the most common agent identified (n = 8 [32%]) followed by ondansetron (n = 3 [12%]). Sixteen of 22 contacted patients were exposed to subsequent anesthesia, including 3 patients with negative evaluations. One patient experienced a mild urticarial HSR.ConclusionInduction agents were the most common causative agents in our patients, which differs from other studies. Given the variability in evaluations of periprocedural HSRs across the United States with data published on small sample sizes, there is a need to establish national guidelines to standardize evaluations and to create a national registry to allow for data sharing.
http://ift.tt/2gpJkAo
Identification and validation of asthma phenotypes in Chinese population using cluster analysis
Publication date: Available online 31 August 2017
Source:Annals of Allergy, Asthma & Immunology
Author(s): Lei Wang, Rui Liang, Ting Zhou, Jing Zheng, Bing Miao Liang, Hong Ping Zhang, Feng Ming Luo, Peter G. Gibson, Gang Wang
BackgroundAsthma is a heterogeneous airway disease, so it is crucial to clearly identify clinical phenotypes to achieve better asthma management.ObjectiveTo identify and prospectively validate asthma clusters in a Chinese population.MethodsTwo hundred eighty-four patients were consecutively recruited and 18 sociodemographic and clinical variables were collected. Hierarchical cluster analysis was performed by the Ward method followed by k-means cluster analysis. Then, a prospective 12-month cohort study was used to validate the identified clusters.ResultsFive clusters were successfully identified. Clusters 1 (n = 71) and 3 (n = 81) were mild asthma phenotypes with slight airway obstruction and low exacerbation risk, but with a sex differential. Cluster 2 (n = 65) described an "allergic" phenotype, cluster 4 (n = 33) featured a "fixed airflow limitation" phenotype with smoking, and cluster 5 (n = 34) was a "low socioeconomic status" phenotype. Patients in clusters 2, 4, and 5 had distinctly lower socioeconomic status and more psychological symptoms. Cluster 2 had a significantly increased risk of exacerbations (risk ratio [RR] 1.13, 95% confidence interval [CI] 1.03–1.25), unplanned visits for asthma (RR 1.98, 95% CI 1.07–3.66), and emergency visits for asthma (RR 7.17, 95% CI 1.26–40.80). Cluster 4 had an increased risk of unplanned visits (RR 2.22, 95% CI 1.02–4.81), and cluster 5 had increased emergency visits (RR 12.72, 95% CI 1.95–69.78). Kaplan-Meier analysis confirmed that cluster grouping was predictive of time to the first asthma exacerbation, unplanned visit, emergency visit, and hospital admission (P < .0001 for all comparisons).ConclusionWe identified 3 clinical clusters as "allergic asthma," "fixed airflow limitation," and "low socioeconomic status" phenotypes that are at high risk of severe asthma exacerbations and that have management implications for clinical practice in developing countries.
http://ift.tt/2wpGNiw
Clinical and biological response to rituximab treatment in 3 patients with acquired C1-inhibitor deficiency
Source:Annals of Allergy, Asthma & Immunology
Author(s): Paula J. Busse, Yael Gernez
http://ift.tt/2gpJ0Sc
Filaggrin mutations and Molluscum contagiosum skin infection in patients with atopic dermatitis
Publication date: Available online 31 August 2017
Source:Annals of Allergy, Asthma & Immunology
Author(s): Sara Manti, Maria Amorini, Caterina Cuppari, Annamaria Salpietro, Francesca Porcino, Salvatore Leonardi, Michele Miraglia Del Giudice, Gianluigi Marseglia, Davide Paolo Caimmi, Carmelo Salpietro
BackgroundAlthough mutations in the filaggrin (FLG) gene have been reported to predispose patients with atopic dermatitis (AD) skin infection susceptibility, to date, the data reported in the literature are still controversial.ObjectiveTo evaluate the role of FLG polymorphisms expression and risk of developing a concomitant Molluscum contagiosum sustained skin infection in the pediatric population with AD.MethodsA total of 100 children with AD and 97 healthy children were enrolled. AD was diagnosed and assessed according to the validated European Task Force on Atopic Dermatitis. DNA samples of patients were analyzed for allelic variants in the promoter and coding exon of FLG. Genotyping was performed with polymerase chain reaction amplification and direct sequencing.ResultsSixteen FLG variants have been detected in 29% of patients with AD: 2 synonymous (rs79808464 and rs116222149), 12 missense (rs11584340, rs113136594, rs145828067, rs374910442, rs747005144, rs145627745, rs144209313, rs74129443, rs192455877, rs150957860, rs138055273, rs147472105), 1 stop gained (rs183942200), and 1 frameshift (rs 558269137). In contrast, only 13% of the control group reported FLG mutations (22 heterozygous variants). In addition, the age at disease onset correlated significantly with FLG variants (P < .001). In addition, the AD with FLG gene variants (rs145627745, rs79808464, rs150957860, rs145828067, rs747005144, rs374910442, rs138055273, rs183942200, rs11584340, and rs113136594) reported moderate to severe Scoring Atopic Dermatitis scores. Finally, the AD group and the AD plus M contagiosum skin infection group had a significant association with FLG mutations when compared with the control group (P < .01).ConclusionFLG mutations are associated with early onset of AD, more severe clinical course of disease, and a significantly increased risk of M contagiosum sustained skin infection.
http://ift.tt/2wYkr9m
Oral melanoma and other pigmentations: when to biopsy?
Abstract
Oral pigmentations (OPs) are often neglected, although a meticulous examination of the oral cavity is important not only in the diagnosis of oral melanoma, but also for the detection of important clinical findings that may indicate the presence of a systemic disease. OPs may be classified into two major groups on the basis of their clinical appearance: focal and diffuse pigmentations, even though this distinction may not appear so limpid in some cases. The former include amalgam tattoo, melanocytic nevi, melanoacanthoma, melanosis, while the latter include physiological/racial pigmentations, smoker's melanosis, drug induced hyperpigmentations, post-inflammatory hyperpigmentations, and OPs associated with systemic diseases.
We will discuss the most frequent OPs and the differential diagnosis with oral mucosal melanoma (OMM), underlining the most frequent lesions that need to undergo a bioptic examination and lesions that could be proposed for a sequential follow-up.
This article is protected by copyright. All rights reserved.
http://ift.tt/2evoIcX
Clinical, pathological and dermoscopic characteristics of cutaneous lesions in LEOPARD syndrome
LEOPARD syndrome (LS) is a rare autosomal disorder associated with multiple congenital anomalies. This term is an acronym for Lentigines (LT), Electrocardiographic conduction defects, Ocular hypertelorism, Pulmonary valve stenosis, Abnormalities of the genitalia, Retardation of growth, and Deafness 1-3. Mutations in PTPN11 gene cause this syndrome in around 90% of cases, and less frequently other mutations produce this entity3-4.
This article is protected by copyright. All rights reserved.
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Parallel ridge dermoscopic pattern in plantar atypical Spitz nevus
Clinicopathological classification, diagnosis and management of spitzoid melanocytic lesion presents one of the most intriguing issues in dermatopathology (1,2). No single clinicopathological feature can offer reliable differentiation of Spitz nevus and melanoma. Although not all the experts agree with the concept of Spitzoid neoplasms as a morpho-biological spectrum, there is a four-tiered classification system proposed by Da Forno et al. and encompassing: 1) Spitz nevus; 2) atypical Spitz nevus; 3) (atypical) Spitz tumor; 4) Spitzoid melanoma (1,3). It is commonly said that Spitz nevus can show all the "local" dermoscopic features of melanoma, but in a more or less tidy fashion (4). The occurrence of melanoma-like dermoscopic pattern in Spitz nevus is also possible (4). The relationship between dermoscopic and histopathologic atypia is not absolute, in as much as dermoscopically atypical lesions are not necessarily histopathologically atypical as well.
This article is protected by copyright. All rights reserved.
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Cetuximab with radiotherapy as an alternative treatment for advanced squamous cell carcinoma of the temporal bone
The prognosis of advanced temporal bone cancer is poor, because complete surgical resection is difficult to achieve. Chemoradiotherapy is one of the available curative treatment options; however, its systemic effects on the patient restrict the use of this treatment. A 69-year-old female (who needed peritoneal dialysis) presented at our clinic with T4 left external auditory canal cancer and was treated with cetuximab plus radiotherapy (RT). The primary lesion showed complete response. The patient is currently alive with no evidence of disease two years after completion of the treatment and does not show any late toxicity.
http://ift.tt/2gvr0t6
Effect of the Visual Information Change in Functional Dystonia
Intervention: Device: prism glasses
Sponsor: Fondation Ophtalmologique Adolphe de Rothschild
Not yet recruiting - verified August 2017
http://ift.tt/2wt0ULb
TTS Esophageal HILZO Stent: A Safety and Feasibility Study
Intervention: Device: HILZO esophageal stent
Sponsors: Radboud University; Kebomed B.V.
Recruiting - verified May 2017
http://ift.tt/2vxI9sW
Observations on fluid flow from exposed dentine in primary teeth: An in vitro study
Source:Archives of Oral Biology, Volume 83
Author(s): Monticha Rangcharoen, Varisara Sirimaharaj, Sitthichai Wanachantararak, Noppakun Vongsavan, Bruce Matthews
ObjectiveTo investigate fluid flow through dentine in primary teeth in vitro using the replica technique, and to compare the results with those obtained from permanent dentine.DesignThe experiments were carried out on 22 extracted, mandibular, primary, incisor teeth. The incisal edge was removed to 1mm below the dentino-enamel junction and half the exposed surface etched with phosphoric acid. The exposed dentine was blotted dry and the pressure in the pulp cavity held at 0, 15, 30 or 45cm H2O above atmospheric for 30s. Fluid that accumulated on the dentine surface was recorded with impression material and a replica made with epoxy resin which was examined in a scanning electron microscope.ResultsStructures resembling fluid droplets were present in the replicas of unetched dentine in all 22 teeth, and at all the pulpal pressures tested. The droplets formed at 45cm H2O were significantly larger (median diam., 5.14mm; interquartile range, 3.26mm; Friedman repeated measures analysis of variance on ranks (RMAVR) and Tukey test) than those formed at other pressures. There was no evidence of droplets in the replicas of etched dentine with any of the pulpal pressures.ConclusionsThese results demonstrate that fluid will tend to flow from dentine in deciduous teeth when it is exposed. They are similar to those obtained in a previous study in this laboratory on permanent teeth. The fact that fluid droplets were absent from etched dentine suggests that, after being blotted, the etched dentine matrix absorbed fluid that tended to flow out through the dentinal tubules.
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Adiponectin prevents orthodontic tooth movement in rats
Publication date: November 2017
Source:Archives of Oral Biology, Volume 83
Author(s): Sigrid Haugen, Kristin Matre Aasarød, Astrid Kamilla Stunes, Mats Peder Mosti, Tanya Franzen, Vaska Vandevska-Radunovic, Unni Syversen, Janne Elin Reseland
ObjectiveThe objective was to examine the effects of repetitive local administration of adiponectin on experimental tooth movement in rats.Materials and methodsThe maxillary right first molar of male Wistar rats (n=24) was moved mesially for 14days, with local adiponectin injections (0.2 or 2μg) every third day. Micro-computed tomography was performed at days 0, 6 and 14 and molar movement, bone density and bone volume fraction were calculated from the scans. Changes in extracellular matrix collagen and cell numbers in the periodontal ligament were analyzed histologically, and levels of circulating cytokines were measured by Luminex and ELISA.ResultsAdiponectin injections induced a reduction in tooth movement after 12 and 14days compared to controls. No tooth movement was observed between days 3 and 14 in the group receiving the highest dosage (2μg) of adiponectin. Differences in bone density and bone volume fractions between treatment and control groups were not identified. Relative size and morphology of collagen fibrils, and cell number in the periodontal region after adiponectin injections were unchanged compared to controls. Levels of circulating adiponectin or other selected factors in plasma were not influenced by the adiponectin injections.ConclusionsSubmucosal injections of adiponectin prevented experimental tooth movement in rats. The effect was dosage-dependent and local. Adiponectin injections caused no detectable changes in bone density, periodontal cell number or collagen content.
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Disseminate recurrent folliculitis as the presenting picture of hidradenitis suppurativa
Source:Annales de Dermatologie et de Vénéréologie
Author(s): J. Revuz
BackgroundHidradenitis suppurativa (HS) has a polymorphous clinical presentation. Herein we report two cases of HS revealed by disseminate recurrent folliculitis.Patients and methods case 1A 31-year-old woman consulted for disseminate recurrent folliculitis on the trunk and proximal segments of the limbs that had been ongoing for several years. No other cutaneous lesions were seen, particularly in the classic area for HS lesions. Weight loss and treatment with azithromycin 500mg/day resulted in complete remission, but relapse occurred on dosage reduction.Case 2The elder sister of patient 1, who was also obese and 10 years her senior, presented a similar clinical history, although in addition, she was presenting lesions characteristic of HS on her armpits and inguinal folds; further, she had undergone an operation 3 years earlier for pilonidal sinus. Three months of combined treatment with rifampicin and clindamycin 900 mg/day followed by long-term doxycycline 200mg/day resulted in marked improvement.DiscussionDisseminate folliculitis is not infrequent during the course of HS; it forms part of the clinical picture and is particularly common in the follicular phenotypic variant; however, it is not part of the diagnostic criteria as defined consensually by the Hidradenitis Suppurativa Foundation. The cases we report suggest that a setting of disseminate recurrent folliculitis may constitute the mode of presentation of HS, and may even represent an atypical monosymptomatic form of HS.ConclusionDisseminate recurrent folliculitis constitutes part of the clinical picture of HS; if it is isolated, screening should be performed for personal or familial signs of HS.
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Evolution of the anterolateral thigh free flap
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The management of pediatric hearing loss caused by auditory neuropathy spectrum disorder
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Increasing access to hearing rehabilitation for older adults
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Brain changes associated with age-related hearing loss
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Perioperative care of head and neck free flap patients
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Hearing health access in developing countries
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Reconstruction of parotidectomy and lateral skull base defects
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Disparities in access to pediatric hearing health care
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Closing the gap in cochlear implant access for African-Americans: a story of outreach and collaboration by our cochlear implant program
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Evaluation and management of cytomegalovirus-associated congenital hearing loss
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Cochlear implantation in children under 12 months of age
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Current assessment of newborn hearing screening protocols
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The expanding role of scapular free-flaps
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Evaluation and management of nonsyndromic congenital hearing loss
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Evaluation and management of syndromic congenital hearing loss
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Advances in midface reconstruction: beyond the reconstructive ladder
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Update on the supraclavicular flap
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Delayed diagnosis and management of second trimester abdominal pregnancy
Second trimester abdominal ectopic pregnancies are rare and life threatening. Early diagnosis and treatment are paramount in reducing maternal morbidity and mortality. We describe an unusually late diagnosis of abdominal pregnancy despite multiple ultrasounds beginning in early pregnancy. A 28-year-old G2P1001 sought pregnancy termination at 22 weeks' gestation after fetal anomalies were noted on an 18-week ultrasound during evaluation for elevated maternal serum alfa-fetoprotein. Due to abortion restrictions in her home state, she travelled over 500 miles for abortion care. During dilation and evacuation, suspected uterine perforation led to the finding of a previously undiagnosed abdominal pregnancy. At laparotomy, she underwent left salpingo-oophorectomy and removal of abdominal pregnancy and placenta. A multidisciplinary team approach was paramount in optimising the patient's outcome. Abortion restrictions requiring travel away from the patient's home community interrupted her continuity of care and created additional hardships, complicating management of an unexpected, rare and life-threatening condition.
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Over-the-scope-clip system secured nasojejunal tube feeding in refractory hyperemesis gravidarum
Hyperemesis gravidarum (HG) is a severe form of nausea and vomiting in pregnancy. It complicates up to 2% of all pregnancies and can be associated with adverse outcomes. Its management commonly involves a combination pharmacotherapy, however, the efficacy of such treatment is limited. Supplemental nutrition is often required in refractory cases. Enteral route is preferred over parenteral, given the high rate of intravenous catheter-related complications. Enteral feeding tube placement can be challenging and is commonly thwarted by dislodgement. We present a pharmacotherapy refractory HG case where the over-the-scope-clip (OTSC) system was successfully used for durable mucosal fixation of the nasojejunal feeding tube.
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Cerebellar abscesses, infective endocarditis and bacteraemia due to a rare pathogen: Streptococcus constellatus
Intracranial infections may result from contiguity, such as mastoiditis or acute otitis media, or from haematogenous dissemination from an infectious source. Streptococcus constellatus, a coccus from the normal genital, oral and gastrointestinal flora, has a tendency to form abscesses, but not to cause infective endocarditis (IE). Also, S. constellatus is an extremely rare causative agent of brain abscess. We report the case of a woman with a colorectal tumour who presented with IE and cerebellar abscesses due to a S. constellatus bacteraemia.
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Wet purpura: a sinister sign in thrombocytopenia
Description
A previously healthy, 38-year-old man presented with non-pruritic rash over his legs and trunk noticed 2 hours back. Examination revealed non-palpable purpura over the limbs and trunk. There was multiple 'wet purpura' over the buccal mucosa, palate and tongue (figure 1A). He had mild pallor and direct ophthalmoscopy showed bilateral fundal haemorrhage with sparing of macula. There was no organomegaly or palpable lymphadenopathy. His investigation showed haemoglobin 125 g/L, white blood cell 8.7x109/L, platelet 7x109/L and normal peripheral smear. He was treated with intravenous methylprednisolone (1 g once a day x3 days) and received one single donor platelet (SDP) transfusion. His bone marrow aspirates showed increased megakaryocytes and no atypical cells or excess of blast; overall picture was consistent with immune thrombocytopenia (ITP). His platelet count on day +3 was 55x109/L. He was put on oral prednisolone (1 mg/kg/day). On day +7, he was asymptomatic with no evidence of any mucocutaneous bleed (figure...
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Massive splenomegaly due to concurrent primary Epstein-Barr virus and cytomegalovirus infection in a patient on adalimumab
A 32-year-old man who was receiving adalimumab for seronegative rheumatoid arthritis presented with a 4-week history of fever, night sweats, fatigue, myalgias and diarrhoea. On examination, he had obvious splenomegaly but no lymphadenopathy or pharyngitis. Full blood count revealed mild neutropenia and significant lymphocytosis, with a blood film showing atypical lymphocytes. Liver function tests were mildly deranged with a mixed hepatitic and obstructive pattern. Ultrasound confirmed massive splenomegaly with a span of 21 cm in the long axis. Serological tests confirmed the presence of both primary Epstein-Barr virus and cytomegalovirus infections. The patient had his adalimumab withheld, was treated with supportive measures and improved over a period of 8 weeks. He remained well 5 months after the onset of illness with complete normalisation of blood count and a resolution of the splenomegaly.
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Correction: Atypical presentation of false asystole detection in implantable loop recorder
Simon Andreas Müggler, Jan Steffel, Ardan Muammer Saguner. Atypical presentation of false asystole detection in implantable loop recorder. BMJ Case Rep (Published Online: 03 Aug 2017). doi: 10.1136/bcr-2017-221280.
The corresponding author's full name is Simon Andreas Müggler.
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SALSA—A dance on a slippery floor with changing partners
Publication date: September 2017
Source:Molecular Immunology, Volume 89
Author(s): M.P. Reichhardt, U. Holmskov, S. Meri
It is becoming increasingly clear that the connections between our immune system and the microbiota colonizing us have a tremendous impact on human health. A number of innate molecular defence mechanisms cooperate to selectively target unwanted microorganisms at the mucosal surfaces. Amongst others these include the complement system, IgA and the SALSA molecule. The salivary scavenger and agglutinin (SALSA), also known as deleted in malignant brain tumors 1 (DMBT1), salivary agglutinin (SAG) or gp340 is a multifunctional molecule with important functions in innate immunity, inflammation and epithelial homeostasis. The SALSA protein is expressed at most mucosal surfaces, where it is one of the most abundant proteins. In the fetal meconium and infant intestine it may constitute even up to 10% of the total protein amount. SALSA is found either directly associated with the epithelial surface or secreted into the lining fluids. In the fluid-phase SALSA interacts with a number of bacterial and viral organisms, as well as with endogenous ligands, including IgA, lactoferrin, surfactant proteins and complement components. While complement has been shown to impact the mucosal environment, this remains an area of limited research. The multiple interactions of the SALSA molecule provide a scaffold, where this potent defence system may engage in cooperative microbial clearance together with corresponding mucosal host ligands.With its high abundance, and multiple effects on both host and microbes, the SALSA molecule is a key player in maintaining the immunological balance at the mucosal surfaces. This is further supported by observations linking the expression of different SALSA isoforms to the development of chronic inflammatory conditions, such as Crohn's disease and ulcerative colitis. This review describes the latest advances in understanding functions of SALSA and its different isoforms. Recently recognized functions are related to complement activation and regulation, endothelial development and epithelial homeostasis. In addition, we suggest mechanisms how SALSA regulates inflammation at the mucosal surfaces.
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Selective expression of the transcription elongation factor ELL3 in B cells prior to ELL2 drives proliferation and survival
Source:Molecular Immunology, Volume 91
Author(s): Lou-Ella M.M. Alexander, January Watters, Jessica A. Reusch, Michelle Maurin, Brook S. Nepon-Sixt, Katerina Vrzalikova, Mark G. Alexandrow, Paul G. Murray, Kenneth L. Wright
B cell activation is dependent on a large increase in transcriptional output followed by focused expression on secreted immunoglobulin as the cell transitions to an antibody producing plasma cell. The rapid transcriptional induction is facilitated by the release of poised RNA pol II into productive elongation through assembly of the super elongation complex (SEC). We report that a SEC component, the Eleven -nineteen Lysine-rich leukemia (ELL) family member 3 (ELL3) is dynamically up-regulated in mature and activated human B cells followed by suppression as B cells transition to plasma cells in part mediated by the transcription repressor PRDM1. Burkitt's lymphoma and a sub-set of Diffuse Large B cell lymphoma cell lines abundantly express ELL3. Depletion of ELL3 in the germinal center derived lymphomas results in severe disruption of DNA replication and cell division along with increased DNA damage and cell death. This restricted utilization and survival dependence reveal a key step in B cell activation and indicate a potential therapeutic target against B cell lymphoma's with a germinal center origin.
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Both Notch1 and its ligands in B cells promote antibody production
Source:Molecular Immunology, Volume 91
Author(s): Gaizhi Zhu, Xiaoqian Wang, He Xiao, Xiaoling Liu, Ying Fang, Bing Zhai, Ruonan Xu, Gencheng Han, Guojiang Chen, Chunmei Hou, Beifen Shen, Yan Li, Ning Ma, Haitao Wu, Guangchao Liu, Renxi Wang
Notch1 signaling regulates B and T lymphocyte development and also in vitro promotes antibody secretion upon B cell activation. However, it is still unclear about the role of Notch1 in antibody production upon in vitro and in vivo mixture lymphocytes activation. We first showed that Notch1 expressed in LPS-activated CD19hi B cells and CD19cre mediated Notch1 knock-down in LPS-activated B cells. Furthermore, we demonstrated that Notch1 knock-down in B cells reduced antibody production in LPS-stimulated B cells but did not affect antibody production in LPS-stimulated splenocytes and in experimental allergic encephalomyelitis (EAE) mice. Importantly, Notch1 ligands Dll1 and Jag1 expressed in B cells and pre-coated Notch1 protein promotes Notch1-knocked down B cells to produce antibody in LPS-stimulated B cells suggesting that Notch1 in other cells may promote antibody production by binding its ligands Dll1 and Jag1 in B cells. Together, our results suggest that both Notch1 and its ligands in B cells play an important role in antibody production.
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NLRC5 promotes cell proliferation via regulating the NF-κB signaling pathway in Rheumatoid arthritis
Source:Molecular Immunology, Volume 91
Author(s): Ya-ru Liu, Xing Yan, Hai-xia Yu, Yao Yao, Jie-quan Wang, Xiao-feng Li, Ruo-nan Chen, Qing-qing Xu, Tao-tao Ma, Cheng Huang, Jun Li
Rheumatoid arthritis (RA) is a chronic autoimmune inflammatory disease and the pathogenesis remains unclear. Previous studies suggested that fibroblast-like synoviocytes (FLSs) play an important role in RA pathogenesis, including the injury of cartilage, the hyperplasia of the synovium and the release of inflammatory cytokines. We used complete Freund's adjuvant (CFA) induced rats as animal models for studying the RA pathogenesis. NLRC5 as the largest member of the NLR family has been reported to play a critical role in regulating immune responses. Increasing evidence suggests that NLRC5 is an pivotal negative modulator of inflammatory pathways. We investigated the mechanisms and signaling pathways of NLRC5 in RA progression. Significantly increased expression of NLRC5 was found in AA rats synovial tissues and cells. And high expression of inflammatory cytokine and cell proliferation of FLSs accompanied with NLRC5 overexpression, but inhibited in cells with NLRC5 silencing treatment. Interestingly, we found that overexpression of NLRC5 also coordinated the activation of NF-κB signaling pathway. These results suggested that NLRC5 promotes RA progression via the NF-κB signaling pathway potentially.
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A novel recombinant vaccine candidate comprising PBP2a and autolysin against Methicillin Resistant Staphylococcus aureus confers protection in the experimental mice
Publication date: November 2017
Source:Molecular Immunology, Volume 91
Author(s): Setareh Haghighat, Seyed Davar Siadat, Seyed Mehdi Rezayat Sorkhabadi, Abbas Akhavan Sepahi, Mehdi Mahdavi
The methicillin-resistant Staphylococcus aureus infection is a hot topic area in microbiology research. Here a novel vaccine candidate consisting of recombinant PBP2a and autolysin proteins were used. The proteins over expressed in E.coli BL21 (DE3) cells, and purified by the Ni-NTA affinity column and conjugated using EDAC and ADH as a linker and spacer, respectively. To investigate the immunogenicity and protective effects of recombinant proteins, 5 and 20μg of proteins in various formulations were subcutaneously injected in different groups. Two booster vaccinations were carried out in three-week intervals and blood samples were collected three weeks after each injection.To evaluate the immune response, total IgG, IgG1, IgG2a, and IgG2b were analyzed. Immunization of mice with r-autolysin and r-autolysin-PBP2a mixture raised total IgGantibody. Additionally, both IgG1 and IgG2a responses induced. Opsonophagocytosis assay showed that anti r-PBP2a and r-autolysin IgG not only promoted phagocytosis of S.aureus, but also decreased the number of viable bacterial cells. Furthermore, survival rate of experimental mice increased in the bacteremia infection. Our results demonstrated that active vaccination with a mixture of r-PBP2a/r-autolysin and conjugate form vaccine reduced the mortality rate and protected mice against lethal MRSA challenge as well as single proteins.
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Preface 16th European Meeting of Complement in Human Disease, Copenhagen, Denmark, September 8th-12th 2017
Publication date: September 2017
Source:Molecular Immunology, Volume 89
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APL-2, a complement C3 inhibitor, may potentially reduce both intravascular and extravascular hemolysis in patients with paroxysmal nocturnal hemoglobinuria
Publication date: September 2017
Source:Molecular Immunology, Volume 89
Author(s): Delphine El Mehdi, Federico V. Grossi, Pascal Deschatelets, Humphrey Pullon, Lisa Tan, Carolina A. Vega, Cedric G. Francois
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Editorial Board/ Publication Information
Publication date: September 2017
Source:Molecular Immunology, Volume 89
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Plasma C4d as marker for lupus nephritis in Systemic Lupus Erythematosus
Publication date: September 2017
Source:Molecular Immunology, Volume 89
Author(s): Myriam Martin, Karolina I. Smolag-Kłosowska, Albin Björk, Birgitta Gullstrand, Marcin Okroj, Jonatan Leffler, Andreas Jönsen, Anders A. Bengtsson, Anna M. Blom
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Genetics of human autoimmunity: From genetic information to functional insights
Source:Clinical Immunology
Author(s): Kazuyoshi Ishigaki, Yuta Kochi, Kazuhiko Yamamoto
Genome-wide association studies have identified hundreds of risk variants associated with human autoimmune diseases. Recent evidence suggests that a substantial portion of them affect gene expression in specific cell types. To obtain the functional insights of GWAS findings, comprehensive characterization of genetic variants in human genome is a key task. In parallel with GWAS, many researches in functional genomics have been conducted in the past decade, and our understandings of cell type-specific gene regulatory system have been substantially improved. In this review, we will introduce the main research topics in functional genomics, and explain their utility to understand biological mechanisms of autoimmune diseases.
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Soluble Interleukin-7 receptor levels and risk of acute graft-versus-disease after allogeneic haematopoietic stem cell transplantation
Publication date: Available online 31 August 2017
Source:Clinical Immunology
Author(s): Katrine Kielsen, Zaiba Shamim, Stephanie Thiant, Sylvie Faucher, Wendy Decker, Ib Jarle Christensen, Lars Peter Ryder, Ibrahim Yakoub-Agha, Klaus Müller
Interleukin-7 is a cytokine essential for T cell homeostasis. IL-7 binds to cellular IL-7 receptors in competition with a soluble form of the receptor (sIL-7Rα). We hypothesized that altered sIL-7Rα levels may cause adverse outcomes in patients undergoing HSCT. In parallel, we investigated the impact of the IL-7Rα SNP rs6897932, which has been associated with release of IL-7R.The sIL-7Rα levels decreased during HSCT (from 114ng/ml before to 48ng/ml at day +14 (P<0.0001)). This pattern was inversely mirrored by IL-7. The IL-7/sIL-7Rα ratio at day +14 was significantly higher in patients developing grade II-IV aGVHD (OR=4.3, P=0.026). Furthermore, donor carriage of the rs6897932 T allele was associated with reduced sIL-7Rα levels, increased risk of grade II-IV aGVHD (OR=2.4, P=0.055) and increased transplant-related mortality (CC=4.5%, CT=21.4% and TT=27.3%, P=0.0037). In conclusion, this study suggests an impact of sIL-7Rα levels and rs6897932 donor genotype on alloreactivity and outcome after HSCT.
Graphical abstract
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SKUP3: 6 and 24 months follow-up of changes in respiration and sleepiness after modified UPPP
Objective
Our previous randomized controlled trial of patients with obstructive sleep apnea syndrome (OSAS) showed that modified uvulopalatopharyngoplasty (UPPP), including tonsillectomy, significantly improved nocturnal respiration, daytime sleepiness, and quality of life in the intervention group compared to controls who had delayed surgery after 6 months. This is the continuous report with the 6- and 24-month postoperative results.
Study Design
Single-center prospective cohort study.
Methods
Sixty-five patients with apnea–hypopnea index (AHI) ≥ 15, body mass index (BMI) < 36, Epworth Sleepiness Scale (ESS) ≥ 8, and Friedman stage I or II underwent UPPP after failing nonsurgical treatment. The results from polysomnography and ESS at 6 and 24 months were compared to baseline.
Results
Eight percent and 20% dropped out from the 6- and 24-month follow-ups, respectively. The AHI value decreased significantly from mean (standard deviation) 52.9 (20.5) at baseline to 23.6 (20.2) after 6 months, and to 24.1 (20.9) after 24 months (P < 0.001). Patients with tonsil size 2, and 3 to 4, had significant reductions in the AHI after both follow-ups. The median ESS score decreased significantly from 13 (range 8–21) to 6.5 (1–18) after 6 months, and to 5 (2–17) after 24 months (P < 0.001). The BMI remained unchanged. There were significant modest correlations for the reductions in AHI and ESS after 24 months.
Conclusion
Modified UPPP was effective in improving nocturnal respiration and daytime sleepiness in OSAS patients at both 6- and 24-month follow-up. Patients with tonsil size 2, and 3 to 4, benefitted similarly from surgery with improved respiration.
Level of Evidence
2b. Laryngoscope, 2017
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Characterization of Head-Trunk Coordination Deficits After Unilateral Vestibular Hypofunction Using Wearable Sensors.
Characterization of Head-Trunk Coordination Deficits After Unilateral Vestibular Hypofunction Using Wearable Sensors.
JAMA Otolaryngol Head Neck Surg. 2017 Aug 31;:
Authors: Paul SS, Dibble LE, Walther RG, Shelton C, Gurgel RK, Lester ME
Abstract
Importance: Individuals with vestibular hypofunction acutely restrict head motion to reduce symptoms of dizziness and nausea. This restriction results in abnormal decoupling of head motion from trunk motion, but the character, magnitude, and persistence of these deficits are unclear.
Objective: To use wearable inertial sensors to quantify the extent of head and trunk kinematic abnormalities in the subacute stage after resection of vestibular schwannoma (VS) and the particular areas of deficit in head-trunk motion.
Design, Setting, and Participants: This cross-sectional observational study included a convenience sample of 20 healthy adults without vestibular impairment and a referred sample of 14 adults 4 to 8 weeks after resection of a unilateral VS at a university and a university hospital outpatient clinic. Data were collected from November 12, 2015, through November 17, 2016.
Exposures: Functional gait activities requiring angular head movements, including items from the Functional Gait Assessment (FGA; range, 1-30, with higher scores indicating better performance), the Timed Up & Go test (TUG; measured in seconds), and a 2-minute walk test (2MWT; measured in meters).
Main Outcomes and Measures: Primary outcomes included peak head rotation amplitude (in degrees), peak head rotation velocity (in degrees per second), and percentage of head-trunk coupling. Secondary outcomes were activity and participation measures including gait speed, FGA score, TUG time, 2MWT distance, and the Dizziness Handicap Inventory score (range, 0-100, with higher scores indicating worse performance).
Results: A total of 34 participants (14 men and 20 women; mean [SD] age, 39.3 [13.6] years) were included. Compared with the 20 healthy participants, the 14 individuals with vestibular hypofunction demonstrated mean (SD) reduced head turn amplitude (84.1° [15.5°] vs 113.2° [24.4°] for FGA-3), reduced head turn velocities (195.0°/s [75.9°/s] vs 358.9°/s [112.5°/s] for FGA-3), and increased head-trunk coupling (15.1% [6.5%] vs 5.9% [5.8%] for FGA-3) during gait tasks requiring angular head movements. Secondary outcomes were also worse in individuals after VS resection compared with healthy individuals, including gait speed (1.09 [0.27] m/s vs 1.47 [0.22] m/s), FGA score (20.5 [3.6] vs 30.0 [0.2]), TUG time (10.9 [1.7] s vs 7.1 [0.8] s), 2MWT (164.8 [37.6] m vs 222.6 [26.8] m), and Dizziness Handicap Inventory score (35.4 [20.7] vs 0.1 [0.4]).
Conclusions and Relevance: With use of wearable sensors, deficits in head-trunk kinematics were characterized along with a spectrum of disability in individuals in the subacute stage after VS surgery compared with healthy individuals. Future research is needed to fully understand how patterns of exposure to head-on-trunk movements influence the trajectory of recovery of head-trunk coordination during community mobility.
PMID: 28859201 [PubMed - as supplied by publisher]
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Natural History and Tumor Volume Kinetics of Papillary Thyroid Cancers During Active Surveillance.
Natural History and Tumor Volume Kinetics of Papillary Thyroid Cancers During Active Surveillance.
JAMA Otolaryngol Head Neck Surg. 2017 Aug 31;:
Authors: Tuttle RM, Fagin JA, Minkowitz G, Wong RJ, Roman B, Patel S, Untch B, Ganly I, Shaha AR, Shah JP, Pace M, Li D, Bach A, Lin O, Whiting A, Ghossein R, Landa I, Sabra M, Boucai L, Fish S, Morris LGT
Abstract
Importance: Active surveillance of low-risk papillary thyroid cancer (PTC) is now an accepted alternative to immediate surgery, but experience with this approach outside of Japan is limited. The kinetics (probability, rate, and magnitude) of PTC tumor growth under active surveillance have not been well defined.
Objective: To describe the kinetics of PTC tumor growth during active surveillance.
Design, Setting, Participants: Cohort study of 291 patients undergoing active surveillance for low-risk PTC (intrathyroidal tumors ≤1.5 cm) with serial tumor measurements via ultrasonography at a tertiary referral center in the United States.
Intervention: Active surveillance.
Main Outcomes and Measures: The cumulative incidence, rate, and magnitude of the change in tumor diameter or volume, as well as associations with patient and tumor characteristics.
Results: Of the 291 patients, 219 (75.3%) were women; mean (SD) age was 52 (15) years. During a median (range) active surveillance of 25 (6-166) months, growth in tumor diameter of 3 mm or more was observed in 11 of 291 (3.8%) patients, with a cumulative incidence of 2.5% (2 years) and 12.1% (5 years). No regional or distant metastases developed during active surveillance. In all cases, 3-dimensional measurements of tumor volume allowed for earlier identification of growth (median, 8.2 months; range, 3-46 months before increase in tumor diameter). In multivariable analysis, both younger age at diagnosis (hazard ratio per year, 0.92; 95% CI, 0.87-0.98; P = .006) and risk category at presentation (hazard ratio for inappropriate, 55.17; 95% CI, 9.4-323.19; P < .001) were independently associated with the likelihood of tumor growth. Of the tumors experiencing volume growth, kinetics demonstrated a classic exponential growth pattern, with a median doubling time of 2.2 years (range, 0.5-4.8 years; median r2 = 0.75; range, 0.42-0.99).
Conclusions and Relevance: The rates of tumor growth during active surveillance in a US cohort with PTCs measuring 1.5 cm or less were low. Serial measurement of tumor volumes may facilitate early identification of tumors that will continue to grow and thereby inform the timing of surveillance imaging and therapeutic interventions.
PMID: 28859191 [PubMed - as supplied by publisher]
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Achieving Active Surveillance for Thyroid Cancer-Not a Euphemism for Watching a Ticking Time Bomb.
Achieving Active Surveillance for Thyroid Cancer-Not a Euphemism for Watching a Ticking Time Bomb.
JAMA Otolaryngol Head Neck Surg. 2017 Aug 31;:
Authors: Scharpf J
PMID: 28859186 [PubMed - as supplied by publisher]
http://ift.tt/2wos5Zl
Simple Tips for Ear Lobule Reconstruction-“Lobuloplasty Revisited”
Abstract
Lobuloplasty is one of the most common procedures done in outpatient basis. It is performed by a wide spectrum of surgeons ranging from general surgeon to plastic surgeon. Inspite of the innumerous techniques used, the ultimate aim of the surgery is to obtain a soft supple lobule with a well-hidden cosmetic scar without the complication of recurrence. Single site local anaesthesia, scoring and undermining of the lobule flaps and the introduction of a small cartilage piece in recurrent cases can change the outcome of the patients result. This article explains how subtle changes are still possible in common procedures like lobuloplasty to change the outcome of the results.
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Evaluation and management of cytomegalovirus-associated congenital hearing loss.
http://ift.tt/2wo7Zyn
Button battery ingestion in children.
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Prospective experience of percutaneous endoscopic gastrostomy tubes placed by otorhinolaryngologist—head and neck surgeons: safe and efficacious
Abstract
Percutaneous endoscopic gastrostomy (PEG) is often the treatment of choice in head and neck cancer (HNC) patients needing long-term nutritional support. Prospective studies on PEG tube placement in an otorhinolaryngologist service are lacking. At our hospital, otolaryngologist—head and neck (ORL-HN) surgeons—have performed PEG insertions for HNC patients since 2008. We prospectively analyzed 127 consecutive HNC patients who received their PEG tubes at the Department of Otorhinolaryngology—head and neck surgery, and evaluated the outcome of PEG tube insertions performed by ORL-HN surgeons. To compare time delays before and after, PEG placement service was transferred from gastrointestinal surgeons to ORL-HN surgeons, and we retrospectively analyzed a separate group of 110 HNC patients who had earlier received PEG tubes at the Department of Gastrointestinal Surgery. ORL-HN surgeons' success rate in PEG insertion was 97.6%, leading to a final prospective study group of 124 patients. Major complications occurred in four (3.2%): two buried bumper syndromes, one subcutaneous hemorrhage leading to an abscess in the abdominal wall, and one metastasis at the PEG site. The most common minor complication was peristomal granulomatous tissue affecting 23 (18.5%) patients. After the change in practice, median time delay before PEG insertion decreased from 13 to 10 days (P < 0.005). The proportion of early PEG placements within 0–3 days increased from 3.6 to 14.6% (P < 0.005). PEG tube insertion seems to be a safe procedure in the hands of an ORL-HN surgeon. Independence from gastrointestinal surgeons' services reduced the time delay and improved the availability of urgent PEG insertions.
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Proximal nail plate destruction in subungual melanoma could be a possible predictor of invasiveness thicker than 1.25 mm
Abstract
Various patterns of nail plate destruction are common features in subungual melanoma (SUM), but there has been no reports regarding their clinical significance according to the pattern of nail plate destruction in terms of tumor thickness. We tried to find a relationship between dermal invasion of SUM and proximal nail plate destruction (PNPD). Clinical information of patients with SUM was reviewed retrospectively from seven dermatology training hospitals in Korea. The PNPD was defined as a visible loss of full thickness of nail plate touching the eponychium at the most proximal part of it. We evaluated whether there are correlations between patients' age, sex, location of SUM, Breslow thickness (BT) and the presence of PNPD. Among 93 patients with SUM, 36 (38.7%) showed PNPD. Sex and BT showed significant correlations with the presence of PNPD in univariate analysis (P < 0.05). BT, age and sex showed significant correlations with the presence of PNPD in multivariate analysis (P < 0.05). Among them, BT showed the strongest correlation with the presence of PNPD (area under the curve, 0.722) and the cut-off value was 1.25 mm. In conclusion, we suggest that PNPD in SUM could be a possible predictor of invasiveness of more than 1.25 mm BT.
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Simple Tips for Ear Lobule Reconstruction-“Lobuloplasty Revisited”
Abstract
Lobuloplasty is one of the most common procedures done in outpatient basis. It is performed by a wide spectrum of surgeons ranging from general surgeon to plastic surgeon. Inspite of the innumerous techniques used, the ultimate aim of the surgery is to obtain a soft supple lobule with a well-hidden cosmetic scar without the complication of recurrence. Single site local anaesthesia, scoring and undermining of the lobule flaps and the introduction of a small cartilage piece in recurrent cases can change the outcome of the patients result. This article explains how subtle changes are still possible in common procedures like lobuloplasty to change the outcome of the results.
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Light-emitting diode aspiration: Distinct radiographic features and approach to management
Publication date: November 2017
Source:International Journal of Pediatric Otorhinolaryngology, Volume 102
Author(s): Jeffrey C. Yeung, C. Jason Smithers, David W. Roberson
Foreign body aspiration is a potentially life threatening problem. The successful removal of airway foreign bodies is dependent on positively identifying the object and developing a solution to its extraction prior to attempts at retrieval. Thus, pre-operative radiographic evaluation is essential in the diagnosis and management of foreign body aspiration. The current case report describes the unique challenges in the evaluation and management of an unusual foreign body within the airway. The distinctive radiographic appearance of this foreign body allows it to be easily identified pre-operatively, and this may decrease the likelihood of operative complications and patient morbidity.
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Evaluation and management of cytomegalovirus-associated congenital hearing loss.
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Button battery ingestion in children.
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Abnormalities of T cell receptor repertoire in CD4+ regulatory and conventional T cells in patients with RAG mutations: implications for autoimmunity
Source:Journal of Allergy and Clinical Immunology
Author(s): Jared H. Rowe, Brian D. Stadinski, Lauren A. Henderson, Lisa Ott de Bruin, Ottavia Delmonte, Yu Nee Lee, M. Teresa de la Morena, Rakesh K. Goyal, Anthony Hayward, Huang Chiung-Hui, Maria Kanariou, Alejandra King, Taco W. Kuijpers, Jian Yi Soh, Benedicte Neven, Jolan E. Walter, Eric S. Huseby, Luigi D. Notarangelo
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The Accuracy of a Handheld Ultrasound Device for Neuraxial Depth and Landmark Assessment: A Prospective Cohort Trial.
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Patients Undergoing Cesarean Delivery After Exposure to Oxytocin During Labor Require Higher Postpartum Oxytocin Doses.
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