Association between serum autotaxin or phosphatidylserine-specific phospholipase A1 levels and melanoma

Abstract

Autotaxin (ATX), a producing enzyme for lysophosphatidic acids, was first identified from the medium of a melanoma cell line and has been considered to be one of the candidate targets to treat melanoma; however, the association between serum ATX and melanoma in human subjects has not been elucidated. Along with ATX, phosphatidylserine-specific phospholipase A1 (PS-PLA₁) is a producing enzyme for lysophosphatidylserine, a similar glycero-lysophospholipid mediator to lysophosphatidic acids. In the present study, we aimed to investigate the association between serum ATX or PS-PLA₁ levels and melanoma. We measured the serum levels of ATX, ATX isoforms and PS-PLA₁ in subjects with melanoma (n = 57) and healthy subjects (n = 58). We further investigated the existence of trends according to the clinical stages of melanoma. We observed that serum total ATX and classical ATX levels were significant higher and serum novel ATX levels tended to be higher in male subjects with melanoma, while no significant difference was observed between the two groups in female subjects. The trend test revealed that the serum total ATX and ATX isoforms were significantly associated with the clinical stages of female subjects with melanoma. Regarding PS-PLA₁, serum PS-PLA₁ levels were significantly higher in the melanoma subjects and associated with the clinical stages. The present study is the first study which revealed the association between ATX or PS-PLA₁ and melanoma, suggesting the possible involvement of ATX/lysophosphatidic acids or PS-PLA₁/lysophosphatidylserine axis in the pathogenesis of melanoma.

http://ift.tt/2CUFCrG

Case of punctate palmoplantar keratoderma type I treated with combination of low-dose oral acitretin and topical salicylic acid and steroid

Abstract

Palmoplantar keratodermas (PPK) are heterogeneous disorders characterized by abnormal keratinization. Especially, punctate PPK (PPPK), one of the subtypes of hereditary PPK, is a rare punctate keratoderma characterized by tiny "raindrop" keratoses having a tendency to coalesce on the edge of soles, which are exposed to sustained pressure. If typical punctate lesions are confined to the palms and soles and the patient has a family history and late onset, it can be considered as PPPK type I (PPKP1), also called Buschke–Fisher–Brauer disease. The exact etiology of PPPK has not been fully understood. Furthermore, no standardized treatment for PPPK has been established and treatment options are limited. Above all, traditional systemic retinoids have been used in several cases, but dose-related adverse effects are common. Therefore, combination of low-dose systemic retinoids and adjuvant topical therapy can be an alternative treatment option for PPPK. Herein, we report a case of PPKP1 treated with combination of low-dose oral acitretin (10 mg/day) and topical salicylic acid and steroid. Despite low capacity, low-dose acitretin showed excellent regression of the lesions by combined use of topical ointments. The supplementary topical therapy may be useful in reducing the dose of systemic retinoids and preventing potential toxicity.

http://ift.tt/2FMOum4

Changes in salivary chromogranin A levels in adults with atopic dermatitis are correlated with changes in their condition

Abstract

Stress-induced scratching is an issue in patients with adult atopic dermatitis (AD). Symptoms of stress-induced AD are common in clinical practise. Salivary chromogranin A (CgA) level has research value as a possible index related to a patient's psychological stress. Using saliva, which is easily collectable, we compared two assessments of the severities of AD and stress with the levels of stress proteins in the saliva of 30 patients with AD in the Department of Dermatology of Shimane University between April 2015 and May 2017. The severities of AD and stress were assessed using the Scoring Atopic Dermatitis (SCORAD) score and State–Trait Anxiety Inventory score, respectively. Additionally, the assessments included those of personality using the Tokyo University Egogram (TEG)-II score and quality of life using the Dermatology Life Quality Index score. Simultaneously, we measured their salivary CgA levels. The change in salivary CgA per protein in patients with AD was correlated with their changes in SCORAD score (correlation coefficient, r = 0.596, P = 0.001) and objective SCORAD (r = 0.608, P < 0.001). The changes in CgA per protein correlated with those in TEG-II A (r = 0.370, P = 0.022), while the changes in SCORAD score correlated with those in DLQI (r = 0.309, P = 0.048). Our results suggest that changes in a patient's condition are reflective of the changes in the patient's stress. The changes in salivary CgA level in patients with AD correlated with the changes in their condition.

http://ift.tt/2CTTUsw

Cutaneous sarcoidosis in a patient with rheumatoid arthritis receiving tocilizumab

http://ift.tt/2FNrJhL

Splice site mutation in COL7A1 resulting in aberrant in-frame transcripts identified in a case of recessive dystrophic epidermolysis bullosa, pretibial

Abstract

Dystrophic epidermolysis bullosa (DEB), pretibial, a rare subtype of epidermolysis bullosa (EB), is characterized by recurrent blisters and erosions predominantly on the pretibial region. We report the case of a 60-year-old Japanese woman with persistent blistering eruptions and scar formation on the pretibial region and elbows. Mutational analysis revealed a previously reported c.5797C>T mutation in exon 70 (p.R1933X) and a novel c.6348+1G>A mutation in intron 76 of COL7A1. Reverse transcription polymerase chain reaction revealed that the c.6348+1G>A mutation resulted in the skipping of exon 76 (69 bp) and the retention of intron 76 (75 bp), and both transcripts were in-frame. From these results, we diagnosed the patient as having recessive DEB, pretibial. A review of previously reported mutations in DEB, pretibial, revealed that one-third of DEB, pretibial, cases showed a recessive inheritance pattern, and no case had a combination of premature termination codon (PTC)/PTC mutations. The DEB, pretibial, case described herein is the first reported case of a compound heterozygote with PTC/in-frame mutations. Although no special characteristic features of the mutations were identified, a high diversity of COL7A1 mutations was shown even in DEB, pretibial.

http://ift.tt/2FbCitV

Overexpression of PIK3CA in head and neck squamous cell carcinoma is associated with poor outcome and activation of the YAP pathway

Publication date: April 2018
Source:Oral Oncology, Volume 79
Author(s): Ramón García-Escudero, Carmen Segrelles, Marta Dueñas, María Pombo, Claudio Ballestín, Marina Alonso-Riaño, Pablo Nenclares, Roberto Álvarez-Rodríguez, Gregorio Sánchez-Aniceto, Ana Ruíz-Alonso, José Luis López-Cedrún, Jesús M. Paramio, Corina Lorz
ObjectivesPhosphatidylinositol 3-kinase catalytic subunit alpha (PIK3CA) is commonly altered in many human tumors, leading to the activation of p110α enzymatic activity that stimulates growth factor-independent cell growth. PIK3CA alterations such as mutation, gene amplification and overexpression are common in head and neck squamous cell carcinoma (HNSCC) and. We aim to explore how these alterations and clinical outcome are associated, as well as the molecular mechanisms involved.Material and methodsMutation and copy-number variation in PIK3CA, and whole-genome expression profiles, were analyzed in primary HNSCC tumors from The Cancer Genome Atlas (TCGA) cohort (n = 243). The results were validated in an independent cohort form the University Hospital of A Coruña (UHAC, n = 62). Expression of the PIK3CA gene protein product (PI3K p110α) and nuclear YAP were assessed in tissue microarrays in a cohort from the University Hospital 12 de Octubre (UH12O, n = 91).ResultsOnly high expression of the PIK3CA gene was associated with poor clinical outcome. The study of gene expression, transcription factor and protein signatures suggested that the activation of the Hippo-YAP pathway, involved in organ size, stem cell maintenance and tumorigenesis, could underlie tumor progression in PI3KCA overexpressing tumors. Tissue arrays showed that PI3K p110α levels correlated with YAP nuclear localization in HNSCC tumors.ConclusionsHigh expression of PIK3CA in HNSCC primary tumors identifies patients at high risk for recurrence. In these tumors, progression could rely on the Hippo-YAP pathway instead of the canonical Akt/mTOR pathway. This observation could have important implications in the therapeutic options for patients.

Graphical abstract

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La azatioprina reduce el riesgo de recaída audiométrica en hipoacusia inmunomediada

Publication date: Available online 2 March 2018
Source:Acta Otorrinolaringológica Española
Author(s): Nieves Mata-Castro, Javier Gavilanes-Plasencia, Rafael Ramírez-Camacho, Alfredo García-Fernández, José Ramón García-Berrocal
IntroducciónLos esquemas actuales de tratamiento de la hipoacusia inmunomediada con corticoides, a dosis baja y pauta corta, son insuficientes.MétodosPara determinar el papel de la azatioprina en el control del deterioro auditivo se ha llevado a cabo un estudio observacional descriptivo longitudinal con 20 pacientes tratados con azatioprina por vía oral (1,5-2,5mg/kg/día en dos dosis) durante 1año. Se consideró recaída la pérdida de 10dB en dos frecuencias consecutivas o de 15dB en una frecuencia aislada.ResultadosLa edad media de los pacientes fue de 52,50años (IC95%: 46,91-58,17), y la mitad fueron mujeres. La afectación bilateral fue del 65%. Un 75% presentaban enfermedad organoespecífica y un 25%, enfermedad autoinmune sistémica. La diferencia entre la PTA basal (46,49 dB; DE18,90) y la PTA a los 12meses (45,47dB; DE18,88) no alcanzó significación estadística (p=0,799). Existía una correlación positiva moderada entre sexo femenino y presencia de enfermedad sistémica (R=0,577). Aplicando t de Student para datos apareados se obtuvo una diferencia significativa (p=0,042) entre el descenso de la PTA en frecuencias hasta 1.000Hz (PTA125-1.000Hz). La tasa relativa de incidencia de recaída por año fue de 0,52 recaídas/año (IC95%: 0,19-1,14). El tiempo medio de supervivencia libre de recaída audiométrica fue de 9,70meses (DE1,03).ConclusionesLa azatioprina mantiene el umbral de audición, disminuye el riesgo de recaída y frena la velocidad con la que los pacientes recaen, alterando el curso de la enfermedad inmunomediada del oído interno.IntroductionCurrent schemes for treatment of immune-mediated hearing loss with sporadic short-course, low-dose corticosteroids, are insufficient.MethodsTo determine the role of azathioprine in the control of auditory impairment, a longitudinal, observational, descriptive study was performed with 20 patients treated with azathioprine (1.5-2.5mg/kg/day into two doses) for 1year. The loss of 10dB on two consecutive frequencies or 15dB on an isolated frequency was considered as relapse.ResultsThe mean age of the patients was 52.50years (95%CI: 46.91-58.17), half were women. Bilateral affectation was 65%. 75% had organ specific disease and 25% had systemic autoimmune disease. The difference between baseline PTA (46.49dB; DS18.90) and PTA at 12months (45.47dB; DS18.88) did not reach statistical significance (P=.799). There was a moderate positive correlation between female sex and the presence of systemic disease (R=.577). By applying Student's t for paired data, a significant difference (P=.042) was obtained between the PTA in frequencies up to 1000 Hz (PTA125-1000Hz). The relative incidence rate of relapse per year was .52 relapses/year (95%CI: .19-1.14]). The median time to audiometric relapse-free was 9.70months (DS1.03).ConclusionsAzathioprine maintains the hearing threshold, decreases the risk of relapse, and slows down the rate at which patients relapse, altering the course of immune-mediated inner ear disease.



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Discriminatory miRNAs for the Management of Papillary Thyroid Carcinoma and Noninvasive Follicular Thyroid Neoplasms with Papillary-Like Nuclear Features

Thyroid , Vol. 0, No. 0.


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Radial nerve injury following dry needling

Robin McManus<br />Jan 26, 2018; 2018:bcr-2017-221302-bcr-2017-221302<br />Case report

http://ift.tt/2oNOha7

Uvulopalatopharyngoplasty vs CN XII stimulation for treatment of obstructive sleep apnea: A single institution experience

Hypoglossal nerve stimulation (HNS) therapy is an emerging surgical treatment for select patients with obstructive sleep apnea (OSA). This study aims to compare outcomes in patients with moderate to severe OSA who underwent HNS surgery (Inspire Medical Systems) and those who underwent traditional airway reconstructive surgery, specifically uvulopalatopharyngoplasty (UPPP).

http://ift.tt/2Febrkv

Partial parotidectomy under local anesthesia for benign parotid tumors - An experience of 50 cases

Benign parotid tumors are adequately treated with partial parotidectomy, which is often performed under general anesthesia (GA). We have reported our preliminary results on the feasibility to perform parotidectomy under local anesthesia (LA). We hereby present our accumulated experience of partial parotidectomy under LA to further consolidate its feasibility, efficacy and safety in a larger series.

http://ift.tt/2CVhrJq

Impact of Topical Fluorouracil Cream on Costs of Treating Keratinocyte Carcinoma (Nonmelanoma Skin Cancer) and Actinic Keratosis

It is unknown whether treatment costs of Keratinocyte Carcinoma (KC) and Actinic Keratosis (AK) can be lowered by spending more on chemoprevention.

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Inflammatory response and cytokine levels induced by intralesional photodynamic therapy and 630nm laser in a case series of basal cell carcinoma

http://ift.tt/2HZaBX5

Modified purse-string closure: a lymphatic channel and tissue sparing technique for biopsy of suspicious pigmented lesions on extremities

http://ift.tt/2oLAsZV

Patients prioritize local recurrence risk over other attributes for surgical treatment of facial melanomas - results of a stated preference survey and choice-based conjoint analysis

Surgical treatment options for facial melanomas include conventional excision with postoperative margin assessment (CE-POMA), Mohs micrographic surgery with immunostains (MMS-I) or slow Mohs. Patient preferences for these surgical options have not been studied.

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Loss of function NFKB1 variants are the most common monogenic cause of CVID in Europeans

Publication date: Available online 2 March 2018
Source:Journal of Allergy and Clinical Immunology
Author(s): Paul Tuijnenburg, Hana Lango Allen, Siobhan O. Burns, Daniel Greene, Machiel H. Jansen, Emily Staples, Jonathan Stephens, Keren J. Carss, Daniele Biasci, Helen Baxendale, Moira Thomas, Anita Chandra, Sorena Kiani-Alikhan, Hilary J. Longhurst, Suranjith L. Seneviratne, Eric Oksenhendler, Ilenia Simeoni, Godelieve J. de Bree, Anton T.J. Tool, Ester M.M. van Leeuwen, Eduard H.T.M. Ebberink, Alexander B. Meijer, Salih Tuna, Deborah Whitehorn, Matthew Brown, Ernest Turro, Adrian J. Thrasher, Kenneth G.C. Smith, James E. Thaventhiran, Taco W. Kuijpers
BackgroundThe genetic etiology of primary immunodeficiency disease (PID) carries prognostic information.ObjectiveWe conducted a whole-genome sequencing study assessing a large proportion of the NIHR-BioResource – Rare Disease cohort.MethodsIn the predominantly European study population of principally sporadic unrelated PID cases (n=846), a novel Bayesian method identified NFKB1 as one most strongly associated with PID, and the association was explained by 16 novel heterozygous truncating, missense and gene deletion variants. This accounted for 4% of common variable immunodeficiency (CVID) cases (n=390) in the cohort. Amino-acid substitutions predicted to be pathogenic were assessed by analysis of structural protein data. Immunophenotyping, immunoblotting and ex vivo stimulation of lymphocytes determined the functional effects of these variants. Detailed clinical and pedigree information was collected for genotype-phenotype co-segregation analyses.ResultsBoth sporadic and familial cases demonstrated evidence of the non-infective complications of CVID, including massive lymphadenopathy (24%), unexplained splenomegaly (48%) and autoimmune disease (48%), features prior studies correlate with worse clinical prognosis. Although partial penetrance of clinical symptoms was noted in certain pedigrees, all carriers have a deficiency in B lymphocyte differentiation. Detailed assessment of B lymphocyte numbers, phenotype and function identifies the presence of a raised CD21^low B cell population: combined with identification of the disease-causing variant, this distinguishes between healthy individuals, asymptomatic carriers and clinically affected cases.ConclusionWe show that heterozygous loss-of-function variants in NFKB1 are the most common known monogenic cause of CVID that results in a temporally progressive defect in the formation of immunoglobulin-producing B cells.

Graphical abstract

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Role of lipid mediators and control of lymphocyte responses in type 2 immunopathology

Publication date: Available online 2 March 2018
Source:Journal of Allergy and Clinical Immunology
Author(s): Sachin K. Samuchiwal, Joshua A. Boyce
Type 2 immunopathology is a cardinal feature of allergic diseases, and involves cooperation between adaptive immunity and innate effector responses. Virtually all cell types relevant to this pathology generate leukotriene and/or prostaglandin mediators that derive from arachidonic acid, and/or express receptors for such mediators. Recent studies highlight prominent functions for these mediators in communication between innate and adaptive immune systems, as well as amplification or suppression of type 2 effector responses. This review focuses on recent advances and insights, and highlights existing and potential therapeutic applications of drugs that target these mediators or their receptors, with a special emphasis on their regulation of the innate and adaptive lymphocytes relevant to type 2 immunopathology.



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Prevalence and clinical challenges among adult primary immunodeficiency patients with RAG deficiency

Publication date: Available online 2 March 2018
Source:Journal of Allergy and Clinical Immunology
Author(s): Dylan Lawless, Christoph B. Geier, Jocelyn R. Farmer, Hana Allen Lango, Daniel Thwaites, Faranaz Atschekzei, Matthew Brown, David Buchbinder, Siobhan O. Burns, Manish J. Butte, Krisztian Csomos, Sri V.V. Deevi, William Egner, Stephan Ehl, Martha M. Eibl, Olajumoke Fadugba, Zsofia Foldvari, Deanna M. Green, Sarah E. Henrickson, Steven M. Holland, Tami John, Christian Klemann, Taco W. Kuijpers, Fernando Moreira, Alexander Piller, Paula Rayner-Matthews, Neil D. Romberg, Ravishankar Sargur, Reinhold E. Schmidt, Claudia Schröder, Catharina Schuetz, Svetlana O. Sharapova, Ken G.C. Smith, Georgios Sogkas, Carsten Speckmann, Kathleen Stirrups, Adrian J. Thrasher, Hermann M. Wolf, Luigi D. Notarangelo, Rashida Anwar, Joan Boyes, Boglarka Ujhazi, James Thaventhiran, Jolan E. Walter, Sinisa Savic




http://ift.tt/2oCDobX

Mast Cell-Derived PAI-1 Promotes Airway Inflammation and Remodeling in a Murine Model of Asthma

Publication date: Available online 2 March 2018
Source:Journal of Allergy and Clinical Immunology
Author(s): Ara Jo, Sun H. Lee, Dong-Young Kim, Seung-Jae Hong, Michael N. Teng, Narasaiah Kolliputi, Richard F. Lockey, Robert P. Schleimer, Seong H. Cho




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Personalized and rapid test for food-related allergy

Publication date: Available online 2 March 2018
Source:Journal of Allergy and Clinical Immunology
Author(s): Mikaël Frossard, Natalia Gasilova, Lionel Arlettaz, Eric Dayer, Hubert H. Girault




http://ift.tt/2FkOEmO

Innate lymphoid cells 3 induce psoriasis in xenotransplanted healthy human skin

Publication date: Available online 2 March 2018
Source:Journal of Allergy and Clinical Immunology
Author(s): Aviad Keren, Avner Shemer, Alex Ginzburg, Yehuda Ullmann, Adam G. Schrum, Ralf Paus, Amos Gilhar




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Epigenome analysis links gene regulatory elements in group 2 innate lymphocytes to asthma susceptibility

Publication date: Available online 2 March 2018
Source:Journal of Allergy and Clinical Immunology
Author(s): Ralph Stadhouders, Bobby W.S. Li, Marjolein J.W. de Bruijn, Antonio Gomez, Tata Nageswara Rao, Hans Jörg Fehling, Wilfred F.J. van IJcken, Ai Ing Lim, James P. Di Santo, Thomas Graf, Rudi W. Hendriks
BackgroundGroup 2 innate lymphoid cells (ILC2s) are major producers of cytokines driving allergic asthma and elevated numbers of ILC2s have been detected in blood and sputum of asthma patients. Asthma susceptibility has a strong genetic component, but the underlying mechanisms and whether asthma genetics impact ILC2 biology remains unclear.ObjectiveTo study the ILC2 transcriptome and epigenome during airway inflammation (AI) in order to couple these to genes and genetic variants associated with asthma pathogenesis.MethodsMice harboring a reporter for the key ILC2 transcription factor GATA3 were subjected to IL-33-driven AI and ILC2s were isolated from bronchoalveolar lavage fluid and mediastinal lymph nodes. Human ILC2s were purified from peripheral blood and activated in vitro. We employed RNA-Seq, genome-wide identification of histone-3 lysine-4 dimethylation (H3K4me2) marked chromatin and computational approaches to study the ILC2 transcriptome and epigenome.ResultsActivated ILC2s in mice displayed a tissue-specific gene expression signature that emerged from remarkably similar epigenomes. We identified superenhancers implicated in controlling ILC2 identity and asthma-associated genes. Over 300 asthma-associated genetic polymorphisms identified in genome-wide association studies localized to H3K4Me2⁺ gene regulatory elements in ILC2s. A refined set of candidate causal asthma-associated variants was uniquely enriched in ILC2 - but not Th2 cell - regulatory regions.ConclusionsILC2s in AI employ a flexible epigenome that couples adaptation to new microenvironments with functional plasticity. Importantly, we reveal strong correlations between gene regulatory mechanisms in ILC2s and the genetic basis of asthma, supporting a pathogenic role for ILC2s in allergic asthma.

Graphical abstract

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Pharmacological Inhibition of Caspase-8 Suppresses Inflammation-Induced Lymphangiogenesis and Allograft Rejection in the Cornea

Publication date: Available online 2 March 2018
Source:Journal of Allergy and Clinical Immunology
Author(s): Wenru Su, Longhui Han, Xiaoqing Chen, Jianfeng Yu, Xiaokang Cheng, Jingwen Huang, Yichen Xiao, Yunzhe Tian, Nancy Olsen, Song Guo Zheng, Dan Liang




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Recurrent Wheezing in Children Following Human Metapneumovirus Infection

Publication date: Available online 2 March 2018
Source:Journal of Allergy and Clinical Immunology
Author(s): Andrea M. Coverstone, Bradley S. Wilson, Dana Burgdorf, Kenneth B. Schechtman, Gregory A. Storch, Michael J. Holtzman, Mario Castro, Leonard B. Bacharier, Kaharu Sumino




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inhaled Corticosteroids in Infants and Toddlers Attenuate Linear Growth

Publication date: Available online 2 March 2018
Source:Journal of Allergy and Clinical Immunology
Author(s): Antti Saari, Lauri J. Virta, Leo Dunkel, Ulla Sankilampi




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New human combined immunodeficiency caused by interferon regulatory factor 4 (IRF4) deficiency inherited by uniparental isodisomy

Publication date: Available online 2 March 2018
Source:Journal of Allergy and Clinical Immunology
Author(s): María Bravo García-Morato, Francisco Javier Aracil Santos, Alejandro Contreras Briones, Alfonso Blázquez Moreno, Ángela del Pozo Maté, Ángeles Domínguez-Soto, María José Beato Merino, Lucía del Pino Molina, Juan Torres Canizales, Ana Victoria Marin, Elena Vallespín García, Marta Feito Rodríguez, Diego Plaza López Sabando, Anaïs Jiménez-Reinoso, Yasmina Mozo del Castillo, Francisco José Sanz Santaeufemia, Raúl de Lucas-Laguna, Paula P. Cárdenas, Laura Casamayor Polo, María Coronel Díaz, Mar Valés-Gómez, Ernesto Roldán Santiago, Antonio Ferreira Cerdán, Julián Nevado Blanco, Ángel L. Corbí, Hugh T. Reyburn, José Ramón Regueiro, Eduardo López-Granados, Rebeca Rodríguez Pena




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A critical role of IL-18 in transformation and maturation of naïve eosinophils to pathogenic eosinophils

Publication date: Available online 2 March 2018
Source:Journal of Allergy and Clinical Immunology
Author(s): Sathisha Upparahalli Venkateshaiah, Akanksha Mishra, Murli Manohar, Alok K. Verma, Priya Rajavelu, Rituraj Niranjan, Laurianne G. Wild, Nereida A. Parada, Uwe Blecker, Joseph A. Lasky, Anil Mishra.

Teaser

The current studies demonstrate a critical role of IL-18 in transforming IL-5 generated naïve eosinophils into the distinct inflammatory CD101⁺CD274⁺ expressing mature and activated tissue eosinophils that promote disease pathogenesis.


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Reply

Publication date: Available online 2 March 2018
Source:Journal of Allergy and Clinical Immunology
Author(s): Masashi Akiyama




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Hidradenitis suppurativa as an autoinflammatory keratinization disease

Publication date: Available online 2 March 2018
Source:Journal of Allergy and Clinical Immunology
Author(s): Valerio De Vita, Dennis McGonagle




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CD40LG duplication-associated autoimmune disease is silenced by non-random X-chromosome inactivation

Publication date: Available online 2 March 2018
Source:Journal of Allergy and Clinical Immunology
Author(s): Carole Le Coz, Melissa Trofa, Camille M. Syrett, Anna Martin, Harumi Jyonouchi, Soma Jyonouchi, Montserrat C. Anguera, Neil Romberg

Teaser

An inherited syndrome of autoimmunity associated with CD40LG duplication is silenced by non-random X-chromosome inactivation and is treatable with CD40L directed therapy.


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Proximity to traffic and asthma among Mexican American children: Independent and interactive effects

Publication date: Available online 2 March 2018
Source:Journal of Allergy and Clinical Immunology
Author(s): Kamal Eldeirawi, Colin Kunzweiler, Natalya Rosenberg, Shannon Zenk, Elizabeth Tarlov, Jennifer Hebert-Beirne, Victoria Persky

Teaser

Our results show deleterious effects of living closer to traffic on respiratory outcomes among Mexican American children. Some of these effects are exacerbated in children with parental history of asthma or allergies.


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SLAM family member 8 is involved in oncogenic KIT-mediated signaling in human mastocytosis

Abstract

The signaling lymphocytic activation molecule family member 8 (SLAMF8)/CD353 is a member of the CD2 family of proteins. Its ligand has not been identified. SLAMF8 is expressed by macrophages and suppresses cellular functions. No study has yet explored SLAMF8 expression or function in human mastocytosis, which features oncogenic KIT-mediated proliferation of human mast cells. SLAMF8 protein was expressed in human mastocytosis cells, immunohistochemically. SLAMF8 expression was also evident in the human mast cell lines, HMC1.2 (expressing oncogenic KIT) and LAD2 (expressing wild-type KIT) cells. SLAMF8-knockdown significantly reduced the KIT-mediated growth of HMC1.2 cells but not that of LAD2 cells. SLAMF8-knockdown HMC1.2 cells exhibited significant attenuation of SHP-2 activation and oncogenic KIT-mediated RAS–RAF–ERK signaling. An interaction between SLAMF8 and SHP-2 was confirmed in HMC1.2 cells and all pathological mastocytosis specimens examined (19 of 19 cases, 100%). Thus, SLAMF8 is involved in oncogenic KIT-mediated RAS–RAF–ERK signaling and the subsequent growth of human neoplastic mast cells mediated by SHP-2. SLAMF8 is a possible therapeutic target in human mastocytosis patients.

This article is protected by copyright. All rights reserved.

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IL-26 in allergic contact dermatitis: resource in a state of readiness

Abstract

In this study, we investigated the role of IL-26 in allergic contact dermatitis (ACD), highlighting its contribute in the cytotoxic mechanism responsible of the tissue injury. IL-26 is a signature Th17 cytokine, and immune cells are its predominant sources. Recently, it has shown that Th17 cell-derived-IL-26 functions like an antimicrobial peptide. Here, we hypothesized that IL-26 could be involved in cytotoxicity mechanism, that underlies ACD. Indeed, we have attributed a role to IL-26 in this context, through PBMC cytotoxicity assays versus Hacat. In order to demonstrate that IL-26 was effectively involved in this activity, we performed the assay using transfected ACD PBMCs by siRNA for IL-26. Indeed, we demonstrated that these cells were less able to kill keratinocytes compared to ACD PBMC (p<0.01). In conclusion, our findings support the idea that this emergent cytokine, IL-26, is implicated in the killing mechanisms of KC observed during ACD.

This article is protected by copyright. All rights reserved.

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Dermal fibroblasts can activate matrix metalloproteinase-1 independent of keratinocytes via plasmin in a 3D collagen model

Abstract

Photoaging of the skin is marked by obvious wrinkles and mainly depends on degradation of the extracellular matrix (ECM) in the dermis. Matrix metalloproteinase (MMP)-1 is one of the most important factors involved in degradation of the ECM, however, its mechanism of activation is not fully understood. It has been thought that MMP-1 is expressed by dermal fibroblasts as an inactive precursor protein that is activated by proteinases produced by keratinocytes in the epidermis. In this study, we constructed a 3D model of the dermis using collagen-embedded fibroblasts with or without ultraviolet (UV)-A exposure to mimic photoaging in the dermis. Collagen lattices embedded with UV-A irradiated fibroblasts miniaturized and collagen was degraded to a greater extent than collagen lattices embedded with non-irradiated fibroblasts. The results demonstrate that fibroblasts in this 3D model express activated-MMP-1 in the absence of keratinocytes. Moreover, the results confirm that activation of MMP-1 depends on increased plasmin activity in this model and lattice miniaturization was inhibited by the plasmin inhibitor tranexamic acid. Our results suggest that plasmin acts as an activator of MMP-1 and the inhibition of plasmin prevents collagen degradation.

This article is protected by copyright. All rights reserved.

http://ift.tt/2t9cvBb

Innate immunity gene expression by epithelial cells of upper respiratory tract in children with adenoid hypertrophy

Publication date: Available online 2 March 2018
Source:Auris Nasus Larynx
Author(s): Lyudmila Viktorovna Gankovskaya, Valentina Pavlovna Bykova, Leila Seimurovna Namasova-Baranova, Alexander Viktorovich Karaulov, Irina Viktorovna Rahmanova, Viktor Anatolievich Gankovskii, Catherine Dmitrievna Merkushova, Oksana Anatolievna Svitich
BackgroundA major role of the innate immunity in the defence of mucosal tissue is well established. However, a balance between the main components of the immunity such as toll-like receptors (TLRs) and defensins in the pathology of upper respiratory tract in children has not been addressed yet. Our aim was to investigate the gene expression of some TLRs as well as alpha and beta-defensins in children suffered from adenoid hyperthrophy in comparison with healthy children.MethodsSamples (nasal epithelium and adenoids) from patients with hypertrophic adenoids (n = 77) and control group (n = 33) were investigated. Quantification of HBD-1 and 2 mRNA, alpha-defensin-HNP1 and toll-like receptors (TLR) 2, 4 and 9 mRNA expression was performed by real-time polymerase chain reaction (PCR). The detection of TLR4 and TLR9 was performed by immunohistochemistry.ResultsThe main finding of the study is a dramatic up-regulation of TLR2 and TLR4 expression (but down-regulation of TLR9) along with a significant reduction in the expression of the defensins in children with adenoid hyperthrophy.ConclusionThe data suggest that one of the mechanisms of mucosal involvement in the pathogenesis of upper respiratory tract infection might by a disbalance between TLRs and defensins revealed in our study.



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Uvulopalatopharyngoplasty vs CN XII stimulation for treatment of obstructive sleep apnea: A single institution experience

Publication date: Available online 2 March 2018
Source:American Journal of Otolaryngology
Author(s): Janki Shah, Jonathon O. Russell, Tina Waters, Alan H. Kominsky, Douglas Trask
ObjectivesHypoglossal nerve stimulation (HNS) therapy is an emerging surgical treatment for select patients with obstructive sleep apnea (OSA). This study aims to compare outcomes in patients with moderate to severe OSA who underwent HNS surgery (Inspire Medical Systems) and those who underwent traditional airway reconstructive surgery, specifically uvulopalatopharyngoplasty (UPPP).MethodsPatients who underwent HNS implantation (n = 20), all with moderate to severe OSA, inability to adhere to positive pressure therapy, and compliant with previously published inclusion criteria, were compared to a historical cohort that were intolerant of CPAP with similar inclusion criteria who all underwent UPPP (n = 20) with some also undergoing additional procedures such as septoplasty/turbinate reduction. Data including body mass index (BMI), pre- and post-implant apnea-hypopnea index (AHI) were assessed.ResultsFor patients who underwent HNS, mean preoperative BMI was 28.0. Mean AHI decreased significantly from 38.9 ± 12.5 to 4.5 ± 4.8. All patients achieved an AHI < 20 post implant with 65% (13/20) with an AHI ≤ 5. For patients who underwent traditional airway surgery, mean preoperative BMI was 27.5; mean AHI decreased from 40.3 ± 12.4 to 28.8 ± 25.4.ConclusionWhile both traditional surgery and HNS are effective treatments for patients with moderate to severe OSA with CPAP intolerance, our study demonstrates that HNS is "curative" in normalizing the AHI to <5 in the majority of patients. For select patients, HNS therapy provides excellent objective improvement in outcome measures.



http://ift.tt/2Fa5IIT

Transoral robotic surgery for the treatment of laryngeal chondrosarcoma: A case report

Publication date: Available online 1 March 2018
Source:American Journal of Otolaryngology
Author(s): Ashley J. Guthrie, Raymond L. Chai
ImportanceTransoral robotic surgery has revolutionized the practice of head and neck surgery over the past decade, with indications now expanding to include laryngeal pathology. Although laryngeal chondrosarcoma is a rare entity, trends in otolaryngology literature suggest that it can frequently be managed with conservative approaches. We hope to inspire other head and neck surgeons to consider transoral robotic surgery for the treatment of such tumors so that outcomes data can be collected and studied.ObservationAn 87-year-old male presented with a left-sided supraglottic mass that was found incidentally on imaging performed for an unrelated reason. Flexible fiberoptic laryngoscopy revealed a cystic-appearing supraglottic mass with near complete obstruction of the glottis airway. Internal review of the imaging demonstrated a 3.5 by 2.3 by 2.8 cm centrally hypodense lesion with a rim of calcification in the left paraglottic space, with resultant narrowing of the laryngeal airway.InterventionThe patient underwent awake tracheostomy and transoral robotic subtotal resection of the mass using the Da Vinci surgical system (Intuitive Surgical Inc., Sunnyvale, CA, USA). The vocal folds, ventricular mucosa, and arytenoid cartilage were grossly preserved. Final pathology revealed a low-grade cartilaginous neoplasm consistent with low-grade chondrosarcoma.Conclusions and relevanceChondrosarcoma of the larynx is a rare entity that accounts for roughly 1% of laryngeal neoplasms. Recent trends in otolaryngology literature and practice suggest that low-grade chondrosarcomas can be effectively treated with organ-preserving, conservative surgery. Transoral robotic surgery offers numerous advantages over both open and endoscopic approaches. To our knowledge, this is the first report in the literature describing the successful use of transoral robotic surgery for the resection of a laryngeal chondrosarcoma.



http://ift.tt/2FNvkwg

Partial parotidectomy under local anesthesia for benign parotid tumors - An experience of 50 cases

Publication date: Available online 2 March 2018
Source:American Journal of Otolaryngology
Author(s): Siu Hong Cheung, Wai Yin Wilson Kwan, Kwan Pok Tsui, Tam Lin Chow
PurposeBenign parotid tumors are adequately treated with partial parotidectomy, which is often performed under general anesthesia (GA). We have reported our preliminary results on the feasibility to perform parotidectomy under local anesthesia (LA). We hereby present our accumulated experience of partial parotidectomy under LA to further consolidate its feasibility, efficacy and safety in a larger series.Materials and methodsCase series review was carried out using our own center patients' database. 50 patients with parotid tumors were treated with partial parotidectomy under LA, using a retrograde nerve dissection approach from January 2006 to October 2016. The inclusion criteria encompassed mobile parotid nodules primarily operated after non-suspicious fine-needle aspiration cytology. Their demographics, complications and outcomes were evaluated.ResultsNo procedure required conversion to GA. There were 48 benign tumors and 2 lymphomas based on the histopathologic examination. The commonest pathologies were pleomorphic adenoma (40%), Warthin's tumor (38%) and cyst (8%). The mean tumor size was 2.68 ± 1.5 cm and the mean operative time was 91.5 ± 34.7 min. Forty-two operations were done as day cases. Ten (20%) patients suffered from transient ear lobe numbness and two (4%) patients had sialocele. Transient facial palsy occurred in 2(4%) patients while another patient (2%) had persistent House-Brackmann grade II facial palsy. No tumor recurrence was noted during follow-up period.ConclusionsPartial parotidectomy adopting a retrograde nerve dissection under LA is feasible and minimally invasive in treating benign parotid tumors. It can avoid the adverse effects of general anesthesia and promote day surgery or minimize hospital stay.



http://ift.tt/2Fa5yBh

SYNERGY-AI: Artificial Intelligence Based Precision Oncology Clinical Trial Matching and Registry

Conditions:   Cancer, Metastatic;   Cancer;   Cancer of Pancreas;   Cancer of Liver;   Cancer of Stomach;   Cancer Liver;   Cancer of Rectum;   Cancer of Kidney;   Cancer of Esophagus;   Cancer of Cervix;   Cancer of Colon;   Cancer of Larynx;   Cancer, Lung;   Cancer, Breast;   Cancer, Advanced;   Cancer Prostate;   Cancer of Neck;   Cancer of Skin;   Neuroendocrine Tumors;   Carcinoma;   Mismatch Repair Deficiency;   BRCA Gene Rearrangement;   Non Hodgkin Lymphoma;   Leukemia;   Non Small Cell Lung Cancer;   Cholangiocarcinoma;   Glioblastoma;   Central Nervous System Tumor;   Melanoma;   Urothelial Carcinoma;   Bladder Cancer;   Ovarian Cancer;   Endometrial Cancer;   Testicular Cancer;   Breast Cancer
Intervention:   Other: Clinical Trial Matching
Sponsor:   Massive Bio, Inc.
Recruiting

http://ift.tt/2GYL4vM

A Study of Atezolizumab (Anti−Pd-L1 Antibody) as Adjuvant Therapy After Definitive Local Therapy in Patients With High-Risk Locally Advanced Squamous Cell Carcinoma of the Head and Neck

Condition:   Locally Advanced Squamous Cell Carcinoma of the Head and Neck (SCCHN)
Interventions:   Drug: Atezolizumab;   Drug: Placebo
Sponsor:   Hoffmann-La Roche
Not yet recruiting

http://ift.tt/2F9bXAP

Three Plastic Rooms of One's Own

Zusi, PA; (2017) Three Plastic Rooms of One's Own. In: Three Plastic Rooms. Jantar Publishing: London, United Kindom.

http://ift.tt/2GYWAaJ

The Icatibant Outcome Survey: experience of hereditary angioedema management from six European countries

Caballero, T; Aberer, W; Longhurst, HJ; Maurer, M; Zanichelli, A; Perrin, A; Bouillet, L; Caballero, T; Aberer, W; Longhurst, HJ; Maurer, M; Zanichelli, A; Perrin, A; Bouillet, L; Andresen, I; - view fewer (2017) The Icatibant Outcome Survey: experience of hereditary angioedema management from six European countries. Journal of the European Academy of Dermatology and Venereology , 31 (7) pp. 1214-1222. 10.1111/jdv.14251 . Green open access

http://ift.tt/2F5OHDJ

Local production of pharmaceuticals and health system strengthening in Africa

Mackintosh, M; Banda, G; Tunguhole, J; (2017) Local production of pharmaceuticals and health system strengthening in Africa. [Review]. German Health Practice Collection

http://ift.tt/2GZZJqW

Magnetic resonance imaging in active surveillance-a modern approach

Giganti, F; Moore, CM; (2018) Magnetic resonance imaging in active surveillance-a modern approach. [Review]. Translational Andrology and Urology , 7 (1) pp. 116-131. 10.21037/tau.2017.12.23 .

http://ift.tt/2oDg1yX

Noncovalent Interactions of pi Systems with Sulfur: The Atomic Chameleon of Molecular Recognition

Motherwell, WB; Moreno, RB; Pavlakos, I; Arendorf, JRT; Arif, T; Tizzard, GJ; Coles, SJ; Motherwell, WB; Moreno, RB; Pavlakos, I; Arendorf, JRT; Arif, T; Tizzard, GJ; Coles, SJ; Aliev, AE; - view fewer (2018) Noncovalent Interactions of pi Systems with Sulfur: The Atomic Chameleon of Molecular Recognition. [Rapid communication]. Angewandte Chemie International Edition , 57 (5) pp. 1193-1198. 10.1002/anie.201708485 .

http://ift.tt/2GZXJP5

Advances and challenges in umbilical cord blood and tissue bioprocessing: procurement and storage

Parker, L; Mansfield, S; Sneddon, K; Charles, B; Rafiq, Q; (2017) Advances and challenges in umbilical cord blood and tissue bioprocessing: procurement and storage. Cell and Gene Therapy Insights , 3 (6) pp. 489-508. 10.18609/cgti.2017.042 . Green open access

http://ift.tt/2F4gZ1D

Computer modelling of halocarbon sorption in zeolite Y

Ramsahye, Naseem Ahmed; (2003) Computer modelling of halocarbon sorption in zeolite Y. Doctoral thesis (Ph.D), UCL (University College London). Green open access

http://ift.tt/2td7s2N

Drop in CD4(+) Counts Below 200 Cells/mu L After Reaching (or Starting From) Values Higher than 350 Cells/mu L in HIV-Infected Patients With Virological Suppression

Gianotti, N; Marchetti, G; Antinori, A; Saracino, A; Gori, A; Rizzardini, G; Lichtner, M; ... Cozzi-Lepri, A; + view all Gianotti, N; Marchetti, G; Antinori, A; Saracino, A; Gori, A; Rizzardini, G; Lichtner, M; Bandera, A; Mussini, C; Girardi, E; Monforte, AD; Cozzi-Lepri, A; - view fewer (2017) Drop in CD4(+) Counts Below 200 Cells/mu L After Reaching (or Starting From) Values Higher than 350 Cells/mu L in HIV-Infected Patients With Virological Suppression. JAIDS: Journal of Acquired Immune Deficiency Syndromes , 76 (4) pp. 417-422. 10.1097/QAI.0000000000001522 .

http://ift.tt/2F6tauL

Harnessing inclusive urban knowledge for the implementation of the New Urban Agenda

Robin, E; Steenmans, K; Acuto, M; (2017) Harnessing inclusive urban knowledge for the implementation of the New Urban Agenda. Urban Research and Practice 10.1080/17535069.2017.1414870 . (In press).

http://ift.tt/2GWzVf1

Discontinuous Galerkin Finite Element Approximation of Nondivergence Form Elliptic Equations With Cordes Coefficients

Smears, I; Sueli, E; (2013) Discontinuous Galerkin Finite Element Approximation of Nondivergence Form Elliptic Equations With Cordes Coefficients. SIAM: Journal on Numerical Analysis , 51 (4) pp. 2088-2106. 10.1137/120899613 . Green open access

http://ift.tt/2F5OvnZ

On the Convergence of Finite Element Methods for Hamilton-Jacobi-Bellman Equations

Jensen, M; Smears, I; (2013) On the Convergence of Finite Element Methods for Hamilton-Jacobi-Bellman Equations. SIAM: Journal on Numerical Analysis , 51 (1) pp. 137-162. 10.1137/110856198 . Green open access

http://ift.tt/2t8W2x3

Acceptability of intranasal live attenuated influenza vaccine, influenza knowledge and vaccine intent in The Gambia

Armitage, E; Camara, J; Bah, S; Forster, AS; Clarke, E; Beate, K; de Silva, T; (2018) Acceptability of intranasal live attenuated influenza vaccine, influenza knowledge and vaccine intent in The Gambia. Vaccine 10.1016/j.vaccine.2018.02.037 . (In press). Green open access

http://ift.tt/2F5Octl

Probing the critical point of the Jaynes–Cummings second-order dissipative quantum phase transition

Mavrogordatos, TK; (2017) Probing the critical point of the Jaynes–Cummings second-order dissipative quantum phase transition. JETP Letters 10.1134/S002136401724002X . (In press).

http://ift.tt/2GX1IMs

Bio-Inspired Octopus Robot Based on Novel Soft Fluidic Actuator

Fras, J; Noh, Y; Maciaś, M; Wurdemann, HA; Althoefer, K; (2018) Bio-Inspired Octopus Robot Based on Novel Soft Fluidic Actuator. In: (Proceedings) IEEE International Conference on Robotics and Automation. IEEE (In press).

http://ift.tt/2F5NUTh

Towards a modular suturing catheter for minimally invasive vascular surgery

Murai, EH; Homer-Vanniasinkam, S; Silveira, PG; Dai, JS; Martins, D; Wurdemann, HA; (2018) Towards a modular suturing catheter for minimally invasive vascular surgery. In: (Proceedings) IEEE International Conference on Robotics and Automation. IEEE (In press).

http://ift.tt/2td7pUF

AirExGlove – A Novel Pneumatic Exoskeleton Glove for Adaptive Hand Rehabilitation in Post-Stroke Patients

Stilli, A; Cremoni, A; Bianchi, M; Ridolf, A; Gerli, F; Vannetti, F; Wurdemann, HA; ... Althoefer, K; + view all Stilli, A; Cremoni, A; Bianchi, M; Ridolf, A; Gerli, F; Vannetti, F; Wurdemann, HA; Allotta, B; Althoefer, K; - view fewer (2018) AirExGlove – A Novel Pneumatic Exoskeleton Glove for Adaptive Hand Rehabilitation in Post-Stroke Patients. In: (Proceedings) IEEE International Conference on Soft Robotics. IEEE (In press).

http://ift.tt/2F3Ezvg

Global science for city policy

Acuto, M; (2018) Global science for city policy. [Editorial comment]. Science , 359 (6372) pp. 165-166. 10.1126/science.aao2728 .

http://ift.tt/2GYWksh

Children's rights and power

Alderson, P; (2017) Children's rights and power. In: Jones, S, (ed.) 30 Years of Social Change. (pp. 80-84). Jessica Kingsley: London, United Kindom.

http://ift.tt/2oC87G2

Crystal structure tuning in organic nanomaterials for fast response and high sensitivity visible-NIR photo-detector

Zou, T; Wang, X; Ju, H; Wu, Q; Guo, T; Wu, W; Wang, H; (2018) Crystal structure tuning in organic nanomaterials for fast response and high sensitivity visible-NIR photo-detector. Journal Of Materials Chemistry C , 6 (6) pp. 1495-1503. 10.1039/c7tc04663d .

http://ift.tt/2thGbfJ

Private sector participation in health care in Zimbabwe: what's the value added?

Mugwagwa, JT; Chinyadza, J; Banda, G; (2017) Private sector participation in health care in Zimbabwe: what's the value added? Journal of Healthcare Communications , 2 (2) , Article 10. 10.4172/2472-1654.100050 . Green open access

http://ift.tt/2oC6msw

Activation of RhoA, Smad2, c-Src, PKC-βII/δ and JNK in atopic dermatitis

Abstract

Atopic dermatitis is a multifactorial skin disease characterised by chronic and relapsing inflammation whose pathogenesis is incompletely understood. We found that the expression of TGFβR1 and the activation of SMAD2, RhoA, JNK, PKC-βII/δ and c-Src were upregulated in the infiltrated inflammatory cells, fibroblasts and vasculatures in the dermis and epidermis. In addition, increases in the expression of TGFβR1 and phosphorylation levels of JNK and c-Src were positively correlated with the inflammatory progression of atopic dermatitis severity.

http://ift.tt/2FOuoYQ

Dental Follicle Mesenchymal Stem Cells Downregulate Th2 Mediated Immune Response in Asthmatic Patients Mononuclear Cells

Abstract

Background

Asthma is a chronic inflammatory disease in which inflammatory responses have the polarization of CD4⁺ T cells to Th2 cells. Dental follicle mesenchymal stem cells (DFSCs) have strong anti-inflammatory properties comparable to other mesenchymal stem cells.

Objective

We investigated the immunomodulatory effects of DFSCs on CD4⁺ T helper cell responses of asthmatic patients and compared the results with those obtained with asthmatic subjects on immunotherapy and with healthy individuals.

Method

Peripheral blood mononuclear cells (PBMC) were isolated from immunotherapy naïve asthmatics, asthmatics on subcutaneous Der p1 immunotherapy and from healthy individuals. PBMC were pre-conditioned with anti-CD3/anti-CD28 mAbs, Der p1 or IFN-γ in the presence and absence of DFSCs and analyzed for T cell viability and proliferation, CD4⁺CD25⁺FOXP3⁺ regulatory T cell frequencies, cytokine expression, and GATA3, T bet and FoxP3 expressions. Neutralization of TGF-β and blockade of IDO and PGE2 pathways were performed to determine suppressive signaling pathways of DFSCs.

Results

DFSCs suppressed proliferative responses of CD4⁺ T lymphocytes and increased the frequency of Treg cells. DFSCs decreased effector and effector memory CD4+ T cell phenotypes in favor of naïve T cell markers. DFSCs decreased IL-4 and GATA3 expression and increased IFN-γ, T-bet and IL-10 expression in asthmatics. Costimulatory molecules were suppressed in monocytes with DFSCs in the cocultures. DFSCs downregulated inflammatory responses via IDO and TGF-β pathways in asthmatic patients.

Conclusion

DFSCs suppressed allergen-induced Th2-cell polarization in favor of Th1 responses and attenuated antigen-presenting cell co-stimulatory activities. These studies suggest that DFSC-based cell therapy may provide pro-tolerogenic immunomodulation relevant to allergic diseases such as asthma.

This article is protected by copyright. All rights reserved.

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Daratumumab augments alloreactive natural killer cell cytotoxicity towards CD38+ multiple myeloma cell lines in a biochemical context mimicking tumour microenvironment conditions

Abstract

Natural killer (NK) cell-based immunotherapy is a promising novel approach to treat cancer. However, NK cell function has been shown to be potentially diminished by factors common in the tumor microenvironment (TME). In this study, we assessed the synergistic potential of antibody-dependent cell-mediated cytotoxicity (ADCC) and killer immunoglobin-like receptor (KIR)-ligand mismatched NK cells to potentiate NK cell antitumor reactivity in multiple myeloma (MM). Hypoxia, lactate, prostaglandin E2 (PGE2) or combinations were selected to mimic the TME. To investigate this, NK cells from healthy donors were isolated and NK cell ADCC capacity in response to MM cells was assessed in flow cytometry-based cytotoxicity and degranulation (CD107a) assays in the presence of TME factors. Hypoxia, lactate and PGE2 reduced cytotoxicity of NK cells against myeloma target cells. The addition of daratumumab (anti-CD38 antibody) augmented NK-cell cytotoxicity against target cells expressing high CD38, but not against CD38 low or negative target cells also in the presence of TME. Co-staining for inhibitory KIRs and NKG2A demonstrated that daratumumab enhanced degranulation of all NK cell subsets. Nevertheless, KIR-ligand mismatched NK cells were slightly better effector cells than KIR-ligand matched NK cells. In summary, our study shows that combination therapy using strategies to maximize activating NK cell signaling by triggering ADCC in combination with an approach to minimize inhibitory signaling through a selection of KIR-ligand mismatched donors, can help to overcome the NK-suppressive TME. This can serve as a platform to improve the clinical efficacy of NK cells.

http://ift.tt/2tcIwbH

Clinical implications of CD4+ T cell subsets in adult atopic asthma patients

T cells play a central role in chronic inflammation in asthma. However, the roles of individual subsets of T cells in the pathology of asthma in patients remain to be better understood.

http://ift.tt/2CUFXug

The Third Institute for Cancer Vaccines and Immunotherapy (ICVI) symposium on immunotherapy: May 12–13, 2017, Royal Society, London, UK

http://ift.tt/2FgLvEJ

Diagnostic and Management Tool for Monitoring Patients Implanted with a Shape-Changing Corneal Inlay

We report two cases that underwent corneal inlay implantation under a femtosecond flap. The first case had no complications during the postoperative period. Pentacam maps remained stable over time: corneal densitometry (CD) values had less than 2 units of change across visits up to 3 years. Axial/sagittal topography maps showed a stable K_max (∼47 D), i.e., less than 1 D change between visits. The second case developed haze at 8 months postoperatively. CD increased by more than 4 units from 3 to 8 M and K_max increased by 2.6 D. The patient was prescribed steroids and 1 month later the haze regressed, resulting in pre-haze CD and K_max values, similar to the ones at 3 months.
Case Rep Ophthalmol 2018;9:190–196

http://ift.tt/2ta0xHE

Amnwiotic Membrane Transplantation as a Treatment for Sterile Infiltration and Corneal Melting after Corneal Crosslinking for Keratoconus

We report a case of corneal melting through sterile infiltration presumably due to excessive use of nonsteroidal anti-inflammatory eye drops after corneal crosslinking (CXL). It was treated using steroids combined with amniotic membrane transplantation (AMT). A 33-year-old man with progressing keratoconus underwent left eye CXL. We prescribed betamethasone and levofloxacin eye drops 5 times daily and diclofenac sodium eye drops 3 times daily for 3 days. Three days after CXL, there was a persistent epithelial defect and the left corneal stroma was clouded. His visual acuity remained unchanged. We prescribed betamethasone hourly and 20 mg prednisolone daily. At 1 week after CXL, the corneal epithelial defect and infiltration were unchanged. He had not stopped using diclofenac at the prescribed time. On day 17, we performed AMT and his cornea and visual acuity improved. AMT may be effective against persistent epithelial defects and corneal melting after CXL.
Case Rep Ophthalmol 2018;9:185–189

http://ift.tt/2F6WAJa

Intraocular Lens Dislocation after Cataract Surgery in Tambolaka, Southwest Sumba, Indonesia: A Case Report

Intraocular lens (IOL) dislocation is a rare complication of cataract extraction requiring prompt surgery. This case report aims to raise awareness of such cases and the importance of post-surgery follow-up. A 58-year-old female patient was found with anterior IOL dislocation a week after phacoemulsification surgery in her right eye. Visual acuity of the right eye was 1/60 with ciliary injection and IOL dislocation to the anterior chamber of the right eye. The patient underwent surgery of the right eye and the IOL haptic was found to be broken. In this case report, the factors affecting IOL dislocation are axis length, broken IOL haptic, and patient activity that increased intraocular pressure. Cataract extraction surgery, although common, needs to be conducted carefully, and it is important for ophthalmologists and general practitioners to detect this condition, especially in rural areas where facilities are limited, as IOL dislocation could occur and requires immediate treatment to achieve a better result.
Case Rep Ophthalmol 2018;9:179–184

http://ift.tt/2tcA8sJ

One-Year Outcome of Aflibercept and Photodynamic Therapy in a Caucasian Patient with Polypoidal Choroidal Vasculopathy Refractory to Ranibizumab and Photodynamic Therapy

Polypoidal choroidal vasculopathy (PCV) is a subtype of neovascular age-related macular degeneration characterised by an abnormal branching vascular network with aneurysmal polypoidal choroidal vascular lesions. PCV is more prevalent in Asian populations than in Caucasians, which may explain its underdiagnosis in Western countries. Evidence regarding the efficacy of different anti-vascular endothelial growth factor (anti-VEGF) agents on PCV is scarce, with most of these studies being conducted in Asian treatment-naïve patients. Ranibizumab was the first anti-VEGF agent to demonstrate the superiority of a combination of photodynamic therapy (PDT) and anti-VEGF over PDT or anti-VEGF monotherapy for inducing polyp regression in Asian patients with PCV. The efficacy of other anti-VEGF agents has been less studied. Resistance to ranibizumab has been described. Aflibercept offers another mechanism of targeting choroidal neovascular lesions. A 75-year-old Caucasian woman presenting to our office was diagnosed with PCV using indocyanine green angiography. Combination therapy with a loading dose of 0.5 mg intravitreal ranibizumab followed by PDT at standard fluence at month 4 and a fourth dose of ranibizumab at month 5 yielded no visual or anatomic outcomes. Treatment was switched to intravitreal aflibercept at month 6 (3 monthly loading doses of 2.0 mg) followed by half-fluence PDT (month 9). Optical coherence tomography revealed remission of the anatomic lesions. Right-eye visual acuity increased to 0.6. Aflibercept injections were administered bimonthly afterwards. Follow-up during 1 year has shown functional and anatomic stability.
Case Rep Ophthalmol 2018;9:172–178

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Posterior Wall Collapse in High Myopia following Cataract Surgery

Purpose: We report a case of posterior globe collapse of an eye after initial recovery from uncomplicated cataract surgery in a patient with high myopia and discuss the course of management involving recognition and emergent air injection with globe reformation. Case Report: A 64-year-old functionally monocular male with a history of high myopia presented for follow-up after uncomplicated cataract surgery. Uncorrected distance visual acuity (UCDVA) at postoperative day 1 was 20/150 with an intraocular pressure (IOP) of 19 mm Hg. At the week 1 visit, UCDVA had decreased to 20/200 with an IOP at 9 mm Hg. After preliminary exam, the keratome site suture was removed, after which the patient reported vision changes. A dilated fundus exam was performed revealing posterior scleral wall collapse. A clinical diagnosis of hypotony was made and a pars plana injection of 1 mL air was performed. This resulted in immediate subjective improvement of vision. Exam the next day revealed UCDVA 20/50 with pinhole improvement to 20/30 and IOP 15 mm Hg. Conclusion: This case demonstrates postoperative hypotony in a patient with pathologic myopia, following cataract surgery. Pathologically myopic eyes may have greater propensity to collapse in the setting of reduced IOP.
Case Rep Ophthalmol 2018;9:167–171

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Erratum

Case Rep Ophthalmol 2018;9:166

http://ift.tt/2F5qaii

Robust Automatic 3D Point Cloud Registration and Object Detection

Selviah, DR; (2018) Robust Automatic 3D Point Cloud Registration and Object Detection. Geomatics World (In press).

http://ift.tt/2CTv1gk

A Nonparametric Revealed Preference Approach to Measuring the Value of Environmental Quality

Blundell, R; Blow, L; (2018) A Nonparametric Revealed Preference Approach to Measuring the Value of Environmental Quality. Environmental and Resource Economics 10.1007/s10640-018-0229-9 . (In press). Green open access

http://ift.tt/2F7gJPc

Epigenome-based cancer risk prediction: rationale, opportunities and challenges

Widschwendter, M; Jones, A; Evans, I; Reisel, D; Dillner, J; Sundström, K; Steyerberg, EW; ... FORECEE (4C) Consortium, .; + view all Widschwendter, M; Jones, A; Evans, I; Reisel, D; Dillner, J; Sundström, K; Steyerberg, EW; Vergouwe, Y; Wegwarth, O; Rebitschek, FG; Siebert, U; Sroczynski, G; de Beaufort, ID; Bolt, I; Cibula, D; Zikan, M; Bjørge, L; Colombo, N; Harbeck, N; Dudbridge, F; Tasse, A-M; Knoppers, BM; Joly, Y; Teschendorff, AE; Pashayan, N; FORECEE (4C) Consortium, .; - view fewer (2018) Epigenome-based cancer risk prediction: rationale, opportunities and challenges. Nature Reviews Clinical Oncology 10.1038/nrclinonc.2018.30 . (In press).

http://ift.tt/2CUkYYx

Heterostructured WO3@CoWO4 bilayer nanosheets for enhanced visible-light photo, electro and photoelectro-chemical oxidation of water

Li, Y; (2018) Heterostructured WO3@CoWO4 bilayer nanosheets for enhanced visible-light photo, electro and photoelectro-chemical oxidation of water. Journal of Materials Chemistry A , 2018 10.1039/C8TA00555A . (In press).

http://ift.tt/2FcBAAb

Quadratic forms and systems of forms in many variables

Rydin Myerson, SL; (2018) Quadratic forms and systems of forms in many variables. Inventiones Mathematicae 10.1007/s00222-018-0789-x . (In press). Green open access

http://ift.tt/2CQqqvr

Abnormal wiring of CCK+ basket cells disrupts spatial information coding

del Pino, I; Brotons-Mas, JR; Marques-Smith, A; Marighetto, A; Frick, A; Marin, O; Rico, B; (2017) Abnormal wiring of CCK+ basket cells disrupts spatial information coding. Nature Neuroscience , 20 (6) pp. 784-792. 10.1038/nn.4544 . Green open access

http://ift.tt/2FgPdOA

Organic remains in late Palaeoproterozoic granular iron formations and implications for the origin of granules

Dodd, MS; Papineau, D; She, Z; Fogel, M; Nederbragt, S; Pirajno, F; (2018) Organic remains in late Palaeoproterozoic granular iron formations and implications for the origin of granules. Precambrian Research (In press).

http://ift.tt/2CRR3jO

When algorithms go wrong, who is liable?

Olhede, S; Wolfe, P; (2017) When algorithms go wrong, who is liable? Significance , 14 (6) pp. 8-9. 10.1111/j.1740-9713.2017.01085.x .

http://ift.tt/2FeC8We

Hierarchically designed hybrid nanoparticles for combinational photochemotherapy against a pancreatic cancer cell line

Joubert, F; Pasparakis, G; (2018) Hierarchically designed hybrid nanoparticles for combinational photochemotherapy against a pancreatic cancer cell line. Journal of Materials Chemistry B , 6 (7) pp. 1095-1104. 10.1039/c7tb03261g . Green open access

http://ift.tt/2CSg8Lq

The Potential of Mobility as a Service Bundles as a Mobility Management Tool

Matyas, MB; Kamargianni, M; (2018) The Potential of Mobility as a Service Bundles as a Mobility Management Tool. In: (Proceedings) 97th Transportation Research Board Annual Meeting. Transportation Research Board: Washington DC, US. Green open access

http://ift.tt/2FgOYTG

Durability of Second Antiretroviral Regimens in the Italian Cohort Naive Antiretrovirals Foundation Study and Factors Associated with Discontinuation

Di Biagio, A; Lorenzini, P; Gustinetti, G; Rusconi, S; De Luca, A; Lapadula, G; Lo Caputo, S; ... Monforte, AD; + view all Di Biagio, A; Lorenzini, P; Gustinetti, G; Rusconi, S; De Luca, A; Lapadula, G; Lo Caputo, S; Cicalini, S; Castelli, F; Marchetti, G; Antinori, A; Monforte, AD; - view fewer (2017) Durability of Second Antiretroviral Regimens in the Italian Cohort Naive Antiretrovirals Foundation Study and Factors Associated with Discontinuation. AIDS Patient Care and STDS , 31 (12) pp. 487-494. 10.1089/apc.2017.0140 .

http://ift.tt/2CRR1bG

Optimized Protocol for Imaging Cleared Neural Tissues Using Light Microscopy

Isogai, Y; Richardson, DS; Dulac, C; Bergan, J; (2016) Optimized Protocol for Imaging Cleared Neural Tissues Using Light Microscopy. Methods in Molecular Biology , 1538 pp. 137-153. 10.1007/978-1-4939-6688-2_11 . Green open access

http://ift.tt/2F7NrjO

Studies of cortical connectivity using optical circuit mapping methods

Anastasiades, PG; Marques-Smith, A; Butt, SJB; (2018) Studies of cortical connectivity using optical circuit mapping methods. [Review]. Journal of Physiology , 596 (2) pp. 145-162. 10.1113/JP273463 .

http://ift.tt/2CRwVhL

Performing the Past: Identity, Civic Culture and Historical Pageants in Twentieth-Century English Small Towns

Bartie, A; Fleming, L; Freeman, M; Hulme, T; Readman, P; (2018) Performing the Past: Identity, Civic Culture and Historical Pageants in Twentieth-Century English Small Towns. In: Small Towns in Europe and Beyond: 20th-21st Century. Karolinum Press, Charles University: Prague, Czech Republic. (In press).

http://ift.tt/2F5Yh9N

An allogeneic 'off the shelf' therapeutic strategy for peripheral nerve tissue engineering using clinical grade human neural stem cells

O'Rourke, C; Day, AGE; Murray-Dunning, C; Thanabalasundaram, L; Cowan, J; Stevanato, L; Grace, N; ... Phillips, JB; + view all O'Rourke, C; Day, AGE; Murray-Dunning, C; Thanabalasundaram, L; Cowan, J; Stevanato, L; Grace, N; Cameron, G; Drake, RAL; Sinden, J; Phillips, JB; - view fewer (2018) An allogeneic 'off the shelf' therapeutic strategy for peripheral nerve tissue engineering using clinical grade human neural stem cells. Scientific Reports , 8 (1) , Article 2951. 10.1038/s41598-018-20927-8 . Green open access

http://ift.tt/2CSCsV9

Personal Informatics Tools Benefit from Combining Automatic and Manual Data Capture in the Long-Term

Ptakauskaite, N; Cox, AL; Musolesi, M; Mehrotra, A; Cheshire, J; Garattini, C; (2018) Personal Informatics Tools Benefit from Combining Automatic and Manual Data Capture in the Long-Term. In: (Proceedings) The ACM Conference on Human Factors in Computing Systems (CHI 2018). (In press).

http://ift.tt/2F5Y0DN

Linkage to HIV care following diagnosis in the WHO European Region: A systematic review and meta-analysis, 2006-2017

Croxford, S; Yin, Z; Burns, F; Copas, A; Town, K; Desai, S; Skingsley, A; ... OptTEST project, ; + view all Croxford, S; Yin, Z; Burns, F; Copas, A; Town, K; Desai, S; Skingsley, A; Delpech, V; OptTEST project, ; - view fewer (2018) Linkage to HIV care following diagnosis in the WHO European Region: A systematic review and meta-analysis, 2006-2017. PLoS One , 13 (2) , Article e0192403. 10.1371/journal.pone.0192403 . Green open access

http://ift.tt/2CUmvOg

Evaluation of food photographs assessing the dietary intake of children up to 10 years old

Valanou, E; Naska, A; Barbouni, A; Katsoulis, M; Peppa, E; Vidalis, P; Trichopoulou, A; (2017) Evaluation of food photographs assessing the dietary intake of children up to 10 years old. Public Health Nutrition 10.1017/S1368980017003354 . (In press).

http://ift.tt/2FiWVrq

Personalisation of warfarin therapy using thermal ink-jet printing

Vuddanda, PR; Alomari, M; Dodoo, CC; Trenfield, SJ; Velaga, S; Basit, AW; Gaisford, S; (2018) Personalisation of warfarin therapy using thermal ink-jet printing. European Journal of Pharmaceutical Sciences , 117 pp. 80-87. 10.1016/j.ejps.2018.02.002 .

http://ift.tt/2CSCcWb

Induced hypothermia in patients with septic shock and respiratory failure (CASS): a randomised, controlled, open-label trial

Itenov, TS; Johansen, ME; Bestle, M; Thormar, K; Hein, L; Gyldensted, L; Lindhardt, A; ... Cooling and Surviving Septic Shock (CASS) Trial Collaboration, .; + view all Itenov, TS; Johansen, ME; Bestle, M; Thormar, K; Hein, L; Gyldensted, L; Lindhardt, A; Christensen, H; Estrup, S; Pedersen, HP; Harmon, M; Soni, UK; Perez-Protto, S; Wesche, N; Skram, U; Petersen, JA; Mohr, T; Waldau, T; Poulsen, LM; Strange, D; Juffermans, NP; Sessler, DI; Tønnesen, E; Møller, K; Kristensen, DK; Cozzi-Lepri, A; Lundgren, JD; Jensen, J-U; Cooling and Surviving Septic Shock (CASS) Trial Collaboration, .; - view fewer (2018) Induced hypothermia in patients with septic shock and respiratory failure (CASS): a randomised, controlled, open-label trial. Lancet Respiratory Medicine , 6 (3) pp. 183-192. 10.1016/S2213-2600(18)30004-3 .

http://ift.tt/2F5kVz8

Reconstruction of a chronically ruptured Achilles tendon using an internal brace: a case report

We reconstructed a chronically ruptured Achilles tendon and the associated scar tissue using braided polyblend polyethylene sutures (FiberWire; Arthrex Inc.; Naples, FL, USA) and anchors.

http://ift.tt/2oCOjCr

Voice Analysis in Post Tonsillectomy Patients

Abstract

The main aim of this study was to analyse the change in voice in terms of acoustic parameters and its perceptual impact in patients who have undergone tonsillectomy. A prospective study was conducted in our institution—JSS Hospital and JSS institute of speech and hearing, Mysore for a duration of 1 year (December 2015–December 2016). 50 post tonsillectomy cases were selected randomly and subjected to acoustic analysis. It was inferred that situation of vocal analysis and assessment for the vowels 'a', 'i' and 'u' under the categories hoarse, harsh and breathy remain more or less the same during preoperative stages, first preoperative follow up and the second post operative follow up. It was concluded that tonsillectomy did not appear to change the acoustic features of vowels remarkably. It was assumed that the subject may adjust the shape of the vocal tract to produce consistent speech sound after surgery using auditory feedback.

http://ift.tt/2oObAkr

Comparison of Endoscopic Turbinoplasty Versus Conventional Partial Inferior Turbinectomy in Chronic Hypertrophic Rhinitis

Abstract

To compare the benefits of endoscopic turbinoplasty and conventional partial inferior turbinectomyin terms of symptomatic relief of the post operative subjective and objective outcomes in patients with chronic hypertrophic rhinitis. A comparative longitudinal study was carried out over a period of 18 months. 60 cases were assigned randomly into two groups—group A (endoscopic turbinoplasty) and group B (conventional partial inferior turbinectomy) and post-operatively were followed up to 6 months to look for postoperative subjective and objective outcomes. Symptomatic improvement in nasal obstruction was found to be consistently better in group A patients at postoperative days 7 and 15, but both groups achieved comparable symptom relief at the third month. A statistically significant difference in SNOT 22 scores were observed between the two groups at all follow-ups. Relatively higher rates of post-operative morbidities were observed in group B at the 7th and 15th postoperative days. Endoscopic turbinoplasty may be better than conventional partial inferior turbinectomy in terms of improvement in nasal obstruction and decreased postoperative morbidity in the immediate postoperative period.

http://ift.tt/2HUafkJ

Barosinusitis: Comprehensive review and proposed new classification system

http://ift.tt/2FIFQVH

Evaluation of a new and simple classification for endoscopic sinus surgery

http://ift.tt/2F8HpLJ

The study of a possible correlation between serum levels of interleukin 17 and clinical severity in patients with allergic rhinitis

http://ift.tt/2FLB1v0

Prenatal maternal depression related to allergic rhinoconjunctivitis in the first 5 years of life in children of the EDEN mother-child cohort study

http://ift.tt/2tbivt5

Characterization of T-cell subpopulations in patients with chronic rhinosinusitis with nasal polyposis

http://ift.tt/2FNZ2l6

A multicenter, prospective, noninterventional study in a Norwegian cohort of patients with moderate-to-severe allergic rhinitis treated with MP-AzeFlu

http://ift.tt/2F6E93e

Oral immunotherapy in children with IgE-mediated hen's egg allergy: Follow-ups at 2.5 and 7 years

http://ift.tt/2FNYTOA

Long-term omalizumab use in the treatment of exercise-induced anaphylaxis

http://ift.tt/2F5AsL7

Long-term follow-up of a patient with malignant transformation of inverted papilloma into sinonasal undifferentiated carcinoma

http://ift.tt/2FMItWM

Multiple doses of icatibant used during pregnancy

http://ift.tt/2F9sHDY

An ergonomic assessment of operating table and surgical stool heights for seated otolaryngology procedures

http://ift.tt/2FKcUwu

Satisfaction rate of patients undergoing sleeve gastrectomy as day-case surgery compared to conventional hospitalization: a prospective non-randomized study

Abstract

Purpose

Day-case surgery (DCS) has boomed over recent years. However, day-case bariatric surgery remains controversial due to a lack of evaluation. The objective of this study was to compare the experiences and satisfaction with general anesthesia of patients undergoing sleeve gastrectomy (SG) as DCS compared to conventional hospitalization.

Methods

Between January 2015 and June 2016, all patients undergoing primary SG as day-case surgery or with conventional hospitalization were prospectively included in this non-randomized, non-inferiority study comparing the level of satisfaction of patients undergoing SG with conventional hospitalization (CH group, gold standard) versus SG as DCS (DCS group). The primary efficacy endpoint was comparison of the overall satisfaction rate using the EVAN-G questionnaire. The secondary endpoints were evaluation of the 6 dimensions of the EVAN-G questionnaire, discharge from hospital, adhesion with SG management and overall satisfaction with SG.

Results

One-hundred and twenty-four patients met the inclusion criteria (62 in both groups). The DCS group was younger with fewer comorbidities (p ≤ 0.01) and had a lower BMI (p ≤ 0.01). Overall, the mean EVAN-G questionnaire score was 66.4 (63.9–68.9) for the DCS group and 68.9 (65.9–71.8) for the CH group (non-inferiority of DCS group). In the DCS group, 19% of patients would have preferred to spend the night in hospital, while 82% of patients in the CH group would have preferred DCS and a total of 75% of patients reported a high level of satisfaction.

Conclusion

Overall satisfaction of patients undergoing SG as day-case surgery was not inferior to that of patients managed by conventional hospitalization.

http://ift.tt/2CTqx9y

Helicobacter pylori and gastric cancer in Alaskan Natives: Time to stop studyingH. pylori and to eradicate it

http://ift.tt/2HWEzuX

Impaired lymphocyte function in patients with hepatic malignancies after selective internal radiotherapy

Abstract

The purpose of our study was to assess the immune function of patients with inoperable hepatic malignancies after treatment with selective internal radiotherapy (SIRT) and to identify possible correlations with clinical parameters. In 25 patients receiving SIRT lymphocyte proliferation and the production of pro- and anti-inflammatory cytokines (interferon-γ and interleukin-10) after stimulation with mitogens and microbial antigens were tested prior to therapy, directly after therapy (day 1) and at day 2, 7 and 28 post therapy using the lymphocyte transformation test and enzyme-linked immunospot assays. Absolute counts and percentages of leukocyte and lymphocyte subsets were determined by flow cytometry. The most prominent finding was an immediate and significant (p < 0.05) decrease of lymphocyte proliferation and interferon-γ production directly after therapy which lasted until day 28 and was stronger upon stimulation with microbial antigens than with mitogens. Moreover, lymphopenia was revealed, affecting all lymphocyte subsets (CD3+, CD4+, CD8+ T cells, CD4+ CD8+ T cells, B cells and NK cells). SIRT led to a reduction in the percentage of activated HLA-DR+ monocytes and of CD45R0+ memory T cells. Higher radiation activity, the presence of liver cirrhosis, chronic kidney disease, diabetes mellitus and metastases were unfavorable factors for immunocompetence, while a better Eastern Cooperative Oncology Group performance status was associated with stronger immunological reactions. In conclusion, SIRT leads to severe impairment of cellular in vitro immune responses. Further studies are needed to assess a potential clinical impact.

http://ift.tt/2oBxCY5

An undergraduate Otolaryngology curriculum comparison in the United Kingdom using a curriculum evaluation framework

http://ift.tt/2oI9z92

Bile acids: a potential role in the pathogenesis of pharyngeal malignancy

http://ift.tt/2HURnSq

Assessment of carotid artery intima‐media thickness in patients with obstructive sleep apnoea

http://ift.tt/2oNRCpY

Single‐sided deafness affects language and auditory development – a case–control study

http://ift.tt/2HWpxW7

Local extension staging of sinonasal tumours: retrospective comparison between CT/MRI assessment and pathological findings

http://ift.tt/2oKuIPV

Adaptation and validation of the Singing Voice Handicap Index into Polish

http://ift.tt/2HUwtD4

An evaluation of the quality of evidence available to inform current bone conducting hearing device national policy

http://ift.tt/2oGhpQG

Effect on mortality of elective parathyroid surgery in one hundred and three patients with chronic kidney disease: our experience

http://ift.tt/2HWpnOv

The value of direct audiology access for magnetic resonance imaging: an audit of 40 cases

http://ift.tt/2oI9su8

Dura and sinus compression with a transcutaneous bone conduction device – hearing outcomes and safety in 38 patients

http://ift.tt/2HW7Upi

Congenital anosmia: our experience of eleven patients with aplasia or hypoplasia of the olfactory tract

http://ift.tt/2oJrRHi

UK tertiary centre experience of outcomes from osseointegrated transcutaneous magnetic bone conduction hearing system implanted in twenty‐five patients using a linear incision technique

http://ift.tt/2HWq627

Short‐term results from seventy‐six patients receiving a bone‐anchored hearing implant installed with a novel minimally invasive surgery technique

http://ift.tt/2oKuzfl

Squamous cell carcinoma associated with inverted papilloma of the maxillary sinus: our experience with 21 patients

http://ift.tt/2HWpRnJ

Functional magnetic resonance imaging in seven borderline cochlear implant candidates: a preliminary research study

http://ift.tt/2oJrtIQ

Balloon tuboplasty in patients with Eustachian tube dysfunction: a prospective study in 39 patients (55 ears)

http://ift.tt/2HVzCm2

A retrospective analysis of seven patients with acquired immunodeficiency syndrome and pharyngeal and/or laryngeal Talaromyces marneffei infection

http://ift.tt/2oJrbSg

Workload and costs of audiological assessment on tertiary settings: Data analysis and audit

http://ift.tt/2HWpwBt

The role of sentinel lymph node biopsy in the management of laryngeal carcinoma

http://ift.tt/2oJgNd3

Insulin effect on hearing recovery in idiopathic sudden sensorineural hearing loss: Retrospective study of 145 patients

http://ift.tt/2HWkAN2

Plant-based diets for children as a means of improving adult cardiometabolic health

Desmond, MA; Sobiecki, J; Fewtrell, MS; Wells, JCK; (2018) Plant-based diets for children as a means of improving adult cardiometabolic health. Nutrition Reviews 10.1093/nutrit/nux079 . (In press).

http://ift.tt/2GSM3xN

Pathogenesis of bat rabies in a natural reservoir: comparative susceptibility of the straw-colored fruit bat (Eidolon helvum) to three strains of Lagos bat virus.

Suu-Ire, R; Begeman, L; Banyard, AC; Breed, AC; Drosten, C; Eggerbauer, E; Freuling, C; ... Cunningham, AA; + view all Suu-Ire, R; Begeman, L; Banyard, AC; Breed, AC; Drosten, C; Eggerbauer, E; Freuling, C; Gibson, L; Goharriz, H; Horton, DL; Jennings, D; Kuzmin, IV; Marston, D; Ntiamoa-Baidu, Y; Sbarbaro, SR; Selden, D; Wise, EL; Kuiken, T; Fooks, AR; Mueller, T; Wood, JLN; Cunningham, AA; - view fewer (2018) Pathogenesis of bat rabies in a natural reservoir: comparative susceptibility of the straw-colored fruit bat (Eidolon helvum) to three strains of Lagos bat virus. PLOS Neglected Tropical Diseases (In press).

http://ift.tt/2FcadpV

High prevalence of chigger mite infection in a forest-specialist frog with evidence of parasite-related granulomatous myositis

Alvarado-Rybak, M; Valenzuela-Sánchez, A; Cevidanes, A; Peñafiel-Ricaurte, A; Uribe-Rivera, D; Cunningham, AA; Soto-Azat, C; (2018) High prevalence of chigger mite infection in a forest-specialist frog with evidence of parasite-related granulomatous myositis. Parasitology Research (In press).

http://ift.tt/2F5ZTfF

Support for viral persistence in bats from age-specific serology and models of maternal immunity

Peel, AJ; Baker, KS; Hayman, DTS; Broder, CC; Cunningham, AA; Fooks, AR; Garnier, R; ... Restif, O; + view all Peel, AJ; Baker, KS; Hayman, DTS; Broder, CC; Cunningham, AA; Fooks, AR; Garnier, R; Wood, JLN; Restif, O; - view fewer (2018) Support for viral persistence in bats from age-specific serology and models of maternal immunity. Scientific Reports , 8 , Article 3859. 10.1038/s41598-018-22236-6 . Green open access

http://ift.tt/2oCosun

Glucocorticoid use and factors associated with variability in this use in the Systemic Lupus International Collaborating Clinics Inception Cohort

Little, J; Parker, B; Lunt, M; Hanly, JG; Urowitz, MB; Clarke, AE; Romero-Diaz, J; ... Bruce, IN; + view all Little, J; Parker, B; Lunt, M; Hanly, JG; Urowitz, MB; Clarke, AE; Romero-Diaz, J; Gordon, C; Bae, S-C; Bernatsky, S; Wallace, DJ; Merrill, JT; Buyon, J; Isenberg, DA; Rahman, A; Ginzler, EM; Petri, M; Dooley, MA; Fortin, P; Gladman, DD; Steinsson, K; Ramsey-Goldman, R; Khamashta, MA; Aranow, C; Mackay, M; Alarcón, GS; Manzi, S; Nived, O; Jönsen, A; Zoma, AA; van Vollenhoven, RF; Ramos-Casals, M; Ruiz-Irastorza, G; Sam Lim, S; Kalunian, KC; Inanc, M; Kamen, DL; Peschken, CA; Jacobsen, S; Askanase, A; Sanchez-Guerrero, J; Bruce, IN; - view fewer (2018) Glucocorticoid use and factors associated with variability in this use in the Systemic Lupus International Collaborating Clinics Inception Cohort. Rheumatology 10.1093/rheumatology/kex444 . (In press).

http://ift.tt/2F6ZlGy

UK's role in global health research innovation

Mannell, J; Abubakar, I; Bastawrous, A; Osrin, D; Patel, P; Piot, P; Prince, M; ... Horton, R; + view all Mannell, J; Abubakar, I; Bastawrous, A; Osrin, D; Patel, P; Piot, P; Prince, M; Smith, J; Wilkinson, RJ; Horton, R; - view fewer (2018) UK's role in global health research innovation. The Lancet , 391 (10122) pp. 721-723. 10.1016/S0140-6736(18)30303-9 .

http://ift.tt/2FddXaF

Rheumatology: Introduction

Isenberg, DA; Rahman, A; (2018) Rheumatology: Introduction. Medicine , 46 (3) p. 151. 10.1016/j.mpmed.2017.12.006 .

http://ift.tt/2F7RQ1T

Jeremy Bentham and Equity: The Court of Chancery, Lord Eldon, and the Dispatch Court Plan

Riley, C; (2018) Jeremy Bentham and Equity: The Court of Chancery, Lord Eldon, and the Dispatch Court Plan. The Journal of Legal History , 39 (1) pp. 29-57. 10.1080/01440365.2018.1434966 .

http://ift.tt/2FjLQGV

Variability and bias in active and passive ground-based measurements of effective plant, wood and leaf area index

Calders, K; Origo, N; Disney, M; Nightingale, J; Woodgate, W; Armston, J; Lewis, P; (2018) Variability and bias in active and passive ground-based measurements of effective plant, wood and leaf area index. Agricultural and Forest Meteorology , 252 pp. 231-240. 10.1016/j.agrformet.2018.01.029 . Green open access

http://ift.tt/2F6YcyK

The Role of Research Evidence in Improving Parliamentary Democracy

Kenny, CA; (2015) The Role of Research Evidence in Improving Parliamentary Democracy. In: Michalek, T and Scherz, C and Hennen, L and Hebáková, L and Hahn, J and Seitz, S, (eds.) The next horizon of technology assessment. (pp. pp. 19-22). Informatorium: Prague, Czech Republic.

http://ift.tt/2oBzd05

Clinical round up

Haddow, L; Herbert, S; (2017) Clinical round up. Sexually Transmitted Infections , 93 (2) p. 148. 10.1136/sextrans-2016-052881 . Green open access

http://ift.tt/2GWs2WW

A multicomponent matched filter cluster confirmation tool for eROSITA: initial application to the RASS and DES-SV data sets

Klein, M; Mohr, JJ; Desai, S; Israel, H; Allam, S; Benoit-Levy, A; Brooks, D; ... Tarle, G; + view all Klein, M; Mohr, JJ; Desai, S; Israel, H; Allam, S; Benoit-Levy, A; Brooks, D; Buckley-Geer, E; Rosell, AC; Kind, MC; Cunha, CE; da Costa, LN; Dietrich, JP; Eifler, TF; Evrard, AE; Frieman, J; Gruen, D; Gruendl, RA; Gutierrez, G; Honscheid, K; James, DJ; Kuehn, K; Lima, M; Maia, MAG; March, M; Melchior, P; Menanteau, F; Miquel, R; Plazas, AA; Reil, K; Romer, AK; Sanchez, E; Santiago, B; Scarpine, V; Schubnell, M; Sevilla-Noarbe, I; Smith, M; Soares-Santos, M; Sobreira, F; Suchyta, E; Swanson, MEC; Tarle, G; - view fewer (2017) A multicomponent matched filter cluster confirmation tool for eROSITA: initial application to the RASS and DES-SV data sets. Monthly Notices of the Royal Astronomical Society (MNRAS) , 474 (3) pp. 3324-3343. 10.1093/mnras/stx2929 . Green open access

http://ift.tt/2Fe6WGC

Enrichment by hybridisation of long DNA fragments for Nanopore sequencing

Eckert, SE; Chan, JZ-M; Houniet, D; PATHSEEK consortium,, ; Breuer, J; Speight, G; (2016) Enrichment by hybridisation of long DNA fragments for Nanopore sequencing. Microbial Genomics , 2 (9) , Article e000087. 10.1099/mgen.0.000087 . Green open access

http://ift.tt/2FdzQXl

COPD exacerbations: transforming outcomes through research.

Hurst, JR; Bafadhel, M; Bolton, CE; Quint, JK; Sapey, E; Wilkinson, TMA; (2018) COPD exacerbations: transforming outcomes through research. [Editorial comment]. Lancet Respir Med , 6 (3) pp. 172-174. 10.1016/S2213-2600(18)30049-3 .

http://ift.tt/2F63cDA

Reasonable Citizens and Epistemic Peers: A Skeptical Problem for Political Liberalism

Van Wietmarschen, JG; (2018) Reasonable Citizens and Epistemic Peers: A Skeptical Problem for Political Liberalism. Journal of Political Philosophy 10.1111/jopp.12152 . (In press).

http://ift.tt/2FdZMC6

Learning to Share: Engineering Adaptive Decision-Support for Online Social Networks

Rafiq, Y; Dickens, L; Russo, A; Bandara, AK; Yang, M; Stuart, A; Levine, M; ... Nuseibeh, B; + view all Rafiq, Y; Dickens, L; Russo, A; Bandara, AK; Yang, M; Stuart, A; Levine, M; Calikli, G; Price, BA; Nuseibeh, B; - view fewer (2017) Learning to Share: Engineering Adaptive Decision-Support for Online Social Networks. In: Rosu, G and DiPenta, M and Nguyen, TN, (eds.) (Proceedings) 32nd IEEE/ACM International Conference on Automated Software Engineering (ASE). (pp. pp. 280-285). IEEE Green open access

http://ift.tt/2GVybmk

Calnexin is necessary for T-cell transmigration into the central nervous system

Jung, J; Eggleton, P; Robinson, A; Wang, J; Gutowski, N; Holley, J; Newcombe, J; ... Michalak, M; + view all Jung, J; Eggleton, P; Robinson, A; Wang, J; Gutowski, N; Holley, J; Newcombe, J; Dudek, E; Paul, AM; Zochodne, D; Kraus, A; Power, C; Agellon, LB; Michalak, M; - view fewer (2018) Calnexin is necessary for T-cell transmigration into the central nervous system. JCI Insight (In press).

http://ift.tt/2oBhIwO

Clinical round-up

Haddow, L; Herbert, S; (2016) Clinical round-up. [Letter]. Sexually Transmitted Infections , 92 (8) p. 632. 10.1136/sextrans-2016-052784 . Green open access

http://ift.tt/2GW44vf

A quorum-sensing system that regulates Streptococcus pneumoniae biofilm formation and surface polysaccharide production

Junges, R; Salvadori, G; Shekhar, S; Åmdal, HA; Periselneris, JN; Chen, T; Brown, JS; Junges, R; Salvadori, G; Shekhar, S; Åmdal, HA; Periselneris, JN; Chen, T; Brown, JS; Petersen, FC; - view fewer (2017) A quorum-sensing system that regulates Streptococcus pneumoniae biofilm formation and surface polysaccharide production. mSphere , 2 (5) , Article e00324-17. 10.1128/mSphere.00324-17 . Green open access

http://ift.tt/2oAL4LW

Demonstrating Clean Burning Future Fuels at a Public Engagement Event

Eveleigh, A; Hellier, P; Karcher, V; Talibi, M; (2017) Demonstrating Clean Burning Future Fuels at a Public Engagement Event. Journal of Chemical Education 10.1021/acs.jchemed.7b00554 . (In press). Green open access

http://ift.tt/2oyDul6