Ebraheim L
from #MedicinebyAlexandrosSfakianakis via xlomafota13 on Inoreader http://ift.tt/1oMyGpD
via IFTTT
Medicine by Alexandros G.Sfakianakis,Anapafseos 5 Agios Nikolaos,Crete 72100,Greece,tel :00302841026182 & 00306932607174
Πέμπτη 18 Φεβρουαρίου 2016
Structural Insights of the Glycogen Body in Domestic Chicken
Mammary Neoplasms in Female Dogs Identification of CytopathologicalCriteria for Malignancy
Kamiguchi IE, Moreira IM, Da Silva Trindade F, Zahn FS, Hataka A, Sousa Rocha N
from #MedicinebyAlexandrosSfakianakis via xlomafota13 on Inoreader http://ift.tt/20Gkevg
via IFTTT
Alterations in Tissue Structure and Morphology in Apo EKO Mice The Impact of Dietary Lipids and Heart Transplantation
Moghadasian MH
from #MedicinebyAlexandrosSfakianakis via xlomafota13 on Inoreader http://ift.tt/1oMyFSM
via IFTTT
The Duplication of the Appendix A Look Crossed Between the Surgeonand the Pathologist
Doh K, Thiam I, Nigobora J, Ndour M, WotoGaye G
from #MedicinebyAlexandrosSfakianakis via xlomafota13 on Inoreader http://ift.tt/20Gk8DZ
via IFTTT
Comparative Effectiveness of Cyanoacrylate Bioadhesives and MonofilamentSuture in Wound Healing A Histopathological and Physicochemical Study inNew Zealand White Rabbit
Angulo AJ, Sebastiaacuten I, Martiacutenez FJ, Torregrosa R, MartiacutenMartiacutenez JM, Madariaga AM
from #MedicinebyAlexandrosSfakianakis via xlomafota13 on Inoreader http://ift.tt/1oMyFCf
via IFTTT
Undoing Gender in Housework? Participation in Domestic Chores by Italian Fathers and Children of Different Ages
Abstract
The present article questions whether and to what extent daughters and sons learn how to "do gender" in housework in Italy, a country with low levels of societal gender equality. Using nationally representative time use survey data from Italy (Italian National Institute of Statistics, 2014, waves 2002–2003 and 2008–2009), where daily time use diaries are collected for entire households, logistic models investigate to what extent children (age 6–12), teenagers (age 13–19), and young adults (ages 20–25) participate in domestic chores and whether paternal involvement in housework (controlling for parental education and employment status) is positively associated with children's participation in domestic chores. The results indicate that daughters are more likely to engage in domestic chores than are sons at all ages and that the gender gap is wider among young adults and teenagers than among children. Moreover, although both sons and daughters are more likely to engage in housework if their father does so, the effect of paternal involvement is much stronger for sons than daughters. These patterns suggest that the learning of housework is a gendered process—a finding that has important implications for the reproduction of gender inequalities in Italy and possibly elsewhere.
from #MedicinebyAlexandrosSfakianakis via xlomafota13 on Inoreader http://ift.tt/1otypbu
via IFTTT
Neurotensin changes propulsive activity into a segmental motor pattern in the rat colon.
| Related Articles |
Neurotensin changes propulsive activity into a segmental motor pattern in the rat colon.
J Neurogastroenterol Motil. 2016 Feb 16;
Authors: Li H, Chen JH, Yang Z, Huang M, Yu Y, Tan S, Luo H, Huizinga JD
Abstract
Background/Aims: Neurotensin is a gut-brain peptide with both inhibitory and excitatory actions on the colonic musculature; our objective was to understand the implications of this for motor patterns occurring in the intact colon of the rat.
Methods: The effects of neurotensin with concentrations ranging from 0.1-100 nM were studied in the intact rat colon in vitro, by investigating spatio-temporal maps created from video recordings of colonic motility before and after neurotensin.
Results: Low concentration of neurotensin (0.1-1 nM) inhibited propagating long distance contractions and rhythmic propagating motor complexes; in its place a slow propagating rhythmic segmental motor pattern developed. The neurotensin receptor 1 antagonist SR-48692 prevented the development of the segmental motor pattern. Higher concentrations of neurotensin (10 nM and 100 nM) were capable of restoring long distance contraction activity and inhibiting the segmental activity. The slow propagating segmental contraction showed a rhythmic contraction-relaxation cycle at the slow wave frequency originating from the interstitial cells of Cajal associated with the myenteric plexus pacemaker. High concentrations given without prior additions of low concentrations did not evoke the segmental motor pattern. These actions occurred when neurotensin was given in the bath solution or intraluminally. The segmental motor pattern evoked by neurotensin was inhibited by the neural conduction blocker lidocaine.
Conclusions: Neurotensin (0.1-1 nM) inhibits the dominant propulsive motor patterns of the colon and a distinct motor pattern of rhythmic slow propagating segmental contractions develops. This motor pattern has the hallmarks of haustral boundary contractions.
PMID: 26882114 [PubMed - as supplied by publisher]
from #MedicinebyAlexandrosSfakianakis via xlomafota13 on Inoreader http://ift.tt/1PTeDN0
via IFTTT
We Care Your Valuable Shipment of Packers and Movers Delhi
They then are usually offering solutions within the area as well as companies for additional significant locations across the nation regarding almost any shifting. They're offering solutions in regards to to home shifting and in many cases as solutions and products in regards to to organization ...
from #MedicinebyAlexandrosSfakianakis via xlomafota13 on Inoreader http://ift.tt/1Q3a0U1
via IFTTT
Fast & Secure Domestic Relocation Servcre in Hydrabad
There're moving companies offering secured and guarded splitting companies from inexpensive price. They have a tendency to be offering providers based on regional splitting within the area, new home buy around the world to all key cities and areas as well as globally splitting for certain areas....
from #MedicinebyAlexandrosSfakianakis via xlomafota13 on Inoreader http://ift.tt/1Q3a0DJ
via IFTTT
Short & Long Term Storage and Moving services in Bangalore
A lot of the Packers along with Movers with Bangalore provide their particular providers with 24*7 conditions. They are often prepared to last. Professional companies have got their exclusive divisions in equipment for organization lovers within towns from the nation. They've got the plants in a...
from #MedicinebyAlexandrosSfakianakis via xlomafota13 on Inoreader http://ift.tt/1Q3a0DF
via IFTTT
Endoluminal loco-regional resection by TEM after R1 endoscopic removal or recurrence of rectal tumors.
| Related Articles |
Endoluminal loco-regional resection by TEM after R1 endoscopic removal or recurrence of rectal tumors.
Minim Invasive Ther Allied Technol. 2016 Feb 16;:1-7
Authors: Quaresima S, Balla A, D'Ambrosio G, Bruzzone P, Ursi P, Lezoche E, Paganini AM
Abstract
Purpose The aim of this study is to evaluate the safety and efficacy of endoluminal loco-regional resection (ELRR) by transanal endoscopic microsurgery (TEM) after R1 endoscopic resection or local recurrence of early rectal cancer after operative endoscopy. Material and methods Twenty patients with early rectal cancer were enrolled, including patients with incomplete endoscopic resection, or complete endoscopic resection of a tumor with unfavorable prognostic factors (group A, ten patients), and local recurrence after endoscopic removal (group B, ten patients). At admission, histology after endoscopic polypectomy was: TisR1(4), T1R0G3(1), T1R1(5) in group A, and TisR0(8), T1R0(2) in group B. All patients underwent ELRR by TEM with nucleotide-guided mesorectal excision (NGME). Results Mean operative time was 150 minutes. Complications occurred in two patients (10%). Definitive histology was: moderate dysplasia(4), pT0N0(3), pTisN0(5), pT1N0(6), pT2N0(2). Mean number of lymph-nodes was 3.1. Mean follow-up was 79.5 months. All patients are alive and disease-free. Conclusions ELRR by TEM after R1 endoscopic resection of early rectal cancer or for local recurrence after operative endoscopy is safe and effective. It may be considered as a diagnostic procedure, as well as a curative treatment option, instead of a more invasive TME.
PMID: 26882538 [PubMed - as supplied by publisher]
from #MedicinebyAlexandrosSfakianakis via xlomafota13 on Inoreader http://ift.tt/1SBpi4E
via IFTTT
Human amniotic epithelial stem cells promote wound healing by facilitating migration and proliferation of keratinocytes via ERK, JNK and AKT signaling pathways
Abstract
Wound healing is a highly orchestrated physiological process consisting in a complex interaction of cellular and biochemical events. Human amniotic epithelial stem cells (HAESCs) have been shown to be an attractive resource for wound healing because they are primitive stem cells. However, the exact effects of amnion-derived stem cells on the migration or proliferation of keratinocytes and their potential mechanism are not fully understood. We have found that HAESCs accelerate the migration of keratinocytes and induce a remarkable increase in the activity of phospho-ERK, phospho-JNK, and phospho-AKT, the blockade of which by their specific inhibitors significantly inhibits migration induced by HAESC-conditioned medium (CM). Furthermore, the co-culture of keratinocytes with HAESCs up-regulates the expression levels of cell proliferation proteins Cyclin D1, Cyclin D3 and Mdm2. In vivo animal experiments have shown that HAESC-CM improves wound healing, whereas blockade with ERK, JNK and AKT inhibitors significantly impairs wound healing. Taken together, these results reveal, for the first time, that HAESCs promote wound healing by facilitating the migration and proliferation of keratinocytes via ERK, JNK and AKT signaling pathways and might be a potential therapy in skin wound healing.
from #MedicinebyAlexandrosSfakianakis via xlomafota13 on Inoreader http://ift.tt/1OhU2Qy
via IFTTT
Hand Preference for a Novel Bimanual Coordinated Task During Termite Feeding in Wild Western Gorillas ( Gorilla gorilla gorilla )
Abstract
Although the level of handedness in humans varies cross-culturally, humans are generally described as right-handed, which has been considered a uniquely human trait. Recently, captive chimpanzees (Pan troglodytes) have been shown to exhibit right-hand preference when performing bimanual but not unimanual tasks. Less clear is whether this pattern also occurs in wild chimpanzees and other African apes. Using videos (N = 49) of six wild western gorillas (Gorilla gorilla gorilla) feeding on termites at the Mondika Research Center (Republic of Congo), we tested whether they exhibit hand preference when performing unimanual, i.e., reaching for termite mound pieces; bimanual, i.e., "termite tapping": rhythmically shaking a piece of termite mound with the dominant hand and collecting the termites in the other hand tasks; or hand transfer prior to bimanual tasks, i.e., transferring a piece of termite mound from one hand to the other. All individuals exhibited exclusive hand preference when performing the bimanual tasks, with five of six gorillas preferring the right hand. Conversely, most individuals did not show any manual preference during the unimanual task. In addition, hand preference during hand transfer revealed clear hand dominance of similar strength and direction of those shown for the bimanual task, suggesting that this measure is as sensitive as the bimanual task itself. Thus, we propose "termite feeding" as a novel task to be considered in future hand-preference studies in wild western gorillas. Our results are in concordance with those for chimpanzees and captive gorillas showing hemispheric specialization for bimanual actions in apes.
from #MedicinebyAlexandrosSfakianakis via xlomafota13 on Inoreader http://ift.tt/1SBnyIE
via IFTTT
Impact of the LSVT on vowel articulation and coarticulation in Parkinson's disease.
| Related Articles |
Impact of the LSVT on vowel articulation and coarticulation in Parkinson's disease.
Clin Linguist Phon. 2015 Jun;29(6):424-40
Authors: Sauvageau VM, Roy JP, Langlois M, Macoir J
Abstract
The purpose of this study was to investigate the impact of the Lee Silverman Voice Treatment (LSVT®) on vowel articulation and consonant-vowel (C-V) coarticulation in dysarthric speakers with Parkinson's disease (PD). Nine Quebec French speakers diagnosed with idiopathic PD underwent the LSVT®. Speech characteristics were compared before and after treatment. Vowel articulation was measured using acoustic vowel space and calculated with the first (F1) and second formant (F2) of the vowels /i/, /u/ and /a/. C-V coarticulation was measured using locus equations, an acoustic metric based on the F2 transitions within vowels in relation to the preceding consonant. The relationship between these variables, speech loudness and vowel duration was also analysed. Results showed that vowel contrast increased in F1/F2 acoustic space after administration of the LSVT®. This improvement was associated with the gain in speech loudness and longer vowel duration. C-V coarticulation patterns between consonant contexts showed greater distinctiveness after the treatment. This improvement was associated with the gain in speech loudness only. These results support the conclusions of previous studies investigating the relationship between the LSVT®, speech loudness and articulation in PD. These results expand clinical understanding of the treatment and indicate that loud speech changes C-V coarticulation patterns. Clinical applications and theoretical considerations are discussed.
PMID: 25688915 [PubMed - indexed for MEDLINE]
from #MedicinebyAlexandrosSfakianakis via xlomafota13 on Inoreader http://ift.tt/1Ua789y
via IFTTT
The effect of age and vocal task on cepstral/spectral measures of vocal function in adult males.
| Related Articles |
The effect of age and vocal task on cepstral/spectral measures of vocal function in adult males.
Clin Linguist Phon. 2015 Jun;29(6):415-23
Authors: Watts CR, Ronshaugen R, Saenz D
Abstract
This study investigated the effect of aging on cepstral/spectral acoustic measures calculated from clinical stimuli (vowels and sentences from the Consensus Auditory Perceptual Evaluation of Voice). Thirty younger adult males (20-49 years of age) and thirty older males (50-79 years of age) produced sustained vowels and read a connected speech stimulus which were applied to cepstral/spectral acoustic analyses to derive the multiparametric measure of Cepstral/Spectral Index of Dysphonia (CSID). Results indicated that older males exhibited significantly greater CSID measures than younger males in connected speech (p=0.001; d=0.98), but not the vowel. Linear regression revealed a moderate correlation between age and CSID in connected speech. These results further inform our understanding of how aging influences voice production in varied contexts and how commonly utilised clinical voice tasks subjected to cepstral/spectral acoustic analyses might differentially inform our knowledge of underlying vocal physiology.
PMID: 25651197 [PubMed - indexed for MEDLINE]
from #MedicinebyAlexandrosSfakianakis via xlomafota13 on Inoreader http://ift.tt/1QLuNIf
via IFTTT
Reflections on clinical expertise and silent know-how in voice therapy.
| Related Articles |
Reflections on clinical expertise and silent know-how in voice therapy.
Logoped Phoniatr Vocol. 2015 Jul;40(2):66-71
Authors: Iwarsson J
Abstract
The concept of 'clinical expertise' is described as a part of evidence-based practice (EBP) together with 'external scientific evidence' and 'patient values and perspectives'. However, clinical expertise in the management of voice disorders has not been described or discussed in much detail. The expertise seems to consist partly of silent know-how that, from the outside, may seem improperly related to the personality of the speech-language pathologist or exclusively dependent on the number of years in the field. In this paper, it is suggested that clinical expertise in voice therapy consists of specific skills that can be explicitly described and trained. These skills are discussed together with educational aspects that contribute to the development of clinical expertise. The skills are also discussed from the perspectives of the past, present, and future.
PMID: 25110954 [PubMed - indexed for MEDLINE]
from #MedicinebyAlexandrosSfakianakis via xlomafota13 on Inoreader http://ift.tt/1QLuNI2
via IFTTT
Mesenchymal Stem/Stromal Cells Derived From a Reproductive Tissue Niche Under Oxidative Stress Have High Aldehyde Dehydrogenase Activity.
| Related Articles |
Mesenchymal Stem/Stromal Cells Derived From a Reproductive Tissue Niche Under Oxidative Stress Have High Aldehyde Dehydrogenase Activity.
Stem Cell Rev. 2016 Feb 15;
Authors: Kusuma GD, Abumaree MH, Pertile MD, Perkins AV, Brennecke SP, Kalionis B
Abstract
The use of mesenchymal stem/stromal cells (MSC) in regenerative medicine often requires MSC to function in environments of high oxidative stress. Human pregnancy is a condition where the mother's tissues, and in particular her circulatory system, are exposed to increased levels of oxidative stress. MSC in the maternal decidua basalis (DMSC) are in a vascular niche, and thus would be exposed to oxidative stress products in the maternal circulation. Aldehyde dehydrogenases (ALDH) are a large family of enzymes which detoxify aldehydes and thereby protect stem cells against oxidative damage. A subpopulation of MSC express high levels of ALDH (ALDH(br)) and these are more potent in repairing and regenerating tissues. DMSC was compared with chorionic villous MSC (CMSC) derived from the human placenta. CMSC reside in vascular niche and are exposed to the fetal circulation, which is in lower oxidative state. We screened an ALDH isozyme cDNA array and determined that relative to CMSC, DMSC expressed high levels of ALDH1 family members, predominantly ALDH1A1. Immunocytochemistry gave qualitative confirmation at the protein level. Immunofluorescence detected ALDH1 immunoreactivity in the DMSC and CMSC vascular niche. The percentage of ALDH(br) cells was calculated by Aldefluor assay and DMSC showed a significantly higher percentage of ALDH(br) cells than CMSC. Finally, flow sorted ALDH(br) cells were functionally potent in colony forming unit assays. DMSC, which are derived from pregnancy tissues that are naturally exposed to high levels of oxidative stress, may be better candidates for regenerative therapies where MSC must function in high oxidative stress environments.
PMID: 26880140 [PubMed - as supplied by publisher]
from #MedicinebyAlexandrosSfakianakis via xlomafota13 on Inoreader http://ift.tt/1SU6P3C
via IFTTT
VATTEL's Le droit des gens
Presentation of Emer de Vattel's Le droit des gens (London [Neuchâtel], 1758), a classic work on the law of nations. Vattel defended the principality of Neuchâtel's interests above all. He tried to combine the natural law tradition (Grotius, Pufendorf, must mostly Wolf, whom he acknowledges being in debt to) and state practice. The result is an often contradictory fundgrube, providing posterity with numerous arguments suitable to any position in trials. Hence Vattel's 19th century popularity and frequent use by the US Supreme court.
from #MedicinebyAlexandrosSfakianakis via xlomafota13 on Inoreader http://ift.tt/1PTeCbO
via IFTTT
Niklas Luhmann: Fashion between the Fashionable and Old-fashioned
The work of German sociologist Niklas Luhmann has been fashionable in the social sciences and humanities since the 1990s. Sociologists, philosophers and literary scholars see the values of his universalistic project to explain all things social. Indeed, his 'super theory' offers a general theory of social systems. Luhmann's project renewed the sociological systems theory tradition through the idea of 'autopoiesis' or self-producing systems, by which he aimed to embrace both constructivism and universalism. Luhmann's thought in a fashion context has been relatively understudied in the field of fashion studies, and Aurélie van der Peer sets out to show how Luhmann's systems theory can offer a fruitful approach to fashion. Rather than explaining in detail Luhmann's framework, which he has gradually developed in over fifty books, she discusses several key ideas that are central to his thought and can be of particular relevance to the study of fashion: modern society as a functionally differentiated social system, the centrality of communication, the rise of fashion as an autonomous and autopoietic subsystem, the operative closure of the fashion subsystem, and its paradoxes. In addition, she wonders whether fashion operates following its own rationality, as every other subsystem does. This observation has the potential to re-address what numerous fashion scholars have argued before: fashion has been unrightfully treated with contempt.
from #MedicinebyAlexandrosSfakianakis via xlomafota13 on Inoreader http://ift.tt/1SBpctN
via IFTTT
Assessing translation and interpretation skills
In this chapter on the assessment of translation and interpreting skills we will briefly outline the origins of translation and interpretation testing and we will review current assessment practices in the educational as well as the professional domain along reliability and validity criteria. In view of the scope of this volume, we will focus on the relationship between language testing and the assessment of translation and interpreting skills. Both translation assessment and interpreting assessment have an obvious link with the measurement of foreign language competence since knowledge of the target language (understanding the source language and mastering the target language) is prerequisite for delivering a successful translation or interpreting performance. However, both translation competence and interpreting competence encompass more than foreign language competence as is evidenced by the definitions of translation and interpreting competence in the literature and the test constructs that have been proposed in reported assessment practices. A sound assessment practice within this domain should therefore take the different dimensions of translation and interpreting competence into account.
from #MedicinebyAlexandrosSfakianakis via xlomafota13 on Inoreader http://ift.tt/1SBpcdd
via IFTTT
Application of FDS to under-ventilated enclosure fires with external flaming
Numerical simulations are conducted to investigate the accuracy of the fire dynamics simulator (FDS 6.0.1) for under-ventilated enclosure fires with external flaming. The accuracy is discussed first in terms of mass balance at the steady-state stage for the enclosure volume. The required fineness of the grid is determined by analyzing the mass balance using two non-dimensional length scales. The first considers the ratio of the ventilation factor to the grid cell size, and the second considers the ratio of the hydraulic diameter of the opening to the grid cell size. When these two length scales are larger than 10, the corresponding mass balance error as obtained from post-processing the output is lower than 4%. The simulation results, including flow through the vertical opening, heat release rate inside the enclosure, gas temperature inside the enclosure, neutral plane height, and external flame height are compared with experimental data and empirical correlations. The air inflow rate through the opening is found to correlate linearly to the ventilation factor as m˙=0.41*A*H^(1/2). For the heat release rate inside the enclosure, the predictions follow the empirical correlation Qin=1131*A*H^(1/2). This is directly related to the air inflow rate and incomplete combustion with the inflowing oxygen. Time-averaged gas temperatures inside the enclosure are under-predicted by maximum 13.1% compared with experimental data at the corner near the opening. Neutral plane height values at the opening, determined from the velocity profile of the vent flow, show good agreement with empirical estimations (Zf=0.4⋅H). Two methods are employed to determine the external flame height, namely a temperature based method and a volume heat release rate based method. The trends are captured correctly.
from #MedicinebyAlexandrosSfakianakis via xlomafota13 on Inoreader http://ift.tt/1PTezwO
via IFTTT
OncomicroRNAs-mediated tumorigenesis: implication in cancer diagnosis and targeted therapy.
| Related Articles |
OncomicroRNAs-mediated tumorigenesis: implication in cancer diagnosis and targeted therapy.
Curr Cancer Drug Targets. 2016 Feb 16;
Authors: Zheng N, Yang P, Wang Z, Zhou Q
Abstract
MicroRNAs (miRNAs) control the expression of approximately 60% of protein-coding genes and regulate cell metabolism, proliferation, differentiation, and apoptosis. Notably, aberrant expression of miRNAs contributes to several diseases including cancer. Accumulating evidence indicates that miRNAs play important roles in EMT, genesis of cancer stem cells, cancer metabolism and carcinogenesis. Aberrant expression of miRNAs triggers tumor initiation，progression and poor prognosis of cancer patients. Accordingly, oncogenic miRNAs have emerged as diagnostic biomarkers and targets for novel anti-cancer drug discovery. However, the mechanisms of miRNAs contriving tumorigenesis are not completely understood. This review aims to clarify the identification of tumor-specific miRNAs, verification of oncogenic miRNA signatures, and dynamic study of oncogenic miRNAs in cancer initiation and development. Despite sound progress in miRNA-mediated anti-cancer therapy, several barriers like drug stability, immunogenicity, off-target effects and toxicities still remain. We hope our review could stimulate the further study of miRNAs in cancer research field, which may lead to new insights into the mechanisms of carcinogenesis and create new avenues for targeted cancer therapy.
PMID: 26881930 [PubMed - as supplied by publisher]
from #MedicinebyAlexandrosSfakianakis via xlomafota13 on Inoreader http://ift.tt/1Wtzien
via IFTTT
E2F1 and NF-κB: Key Mediators of Inflammation-associated Cancers and Potential Therapeutic Targets.
| Related Articles |
E2F1 and NF-κB: Key Mediators of Inflammation-associated Cancers and Potential Therapeutic Targets.
Curr Cancer Drug Targets. 2016 Feb 16;
Authors: Huang Y, Chen R, Zhou J
Abstract
Inflammation is the fundamental protective immuno-response and frequently occurred in organisms including humen beings. However, disordered response can cause chronic human disease, including cancer. Inflammatory cells and mediators are essential to the tumor microenvironment, and dissection of this complex molecular and cellular milieu may elucidate a connection between cancer and inflammation. Thus, focusing on transcription factor NF-κB and E2F1 in inflammation-associated cancer is urgent. NF-κB activation is prevalent in carcinomas, mainly driven by inflammatory cytokines in the tumor microenvironment. E2F1 is also involved in regulating immune responses. Understanding the crosstalk between the two pathways and the behind regulating mechanisms may contribute to the development of novel anti-cancer therapeutics.
PMID: 26881929 [PubMed - as supplied by publisher]
from #MedicinebyAlexandrosSfakianakis via xlomafota13 on Inoreader http://ift.tt/1SBft6Q
via IFTTT
Significance and therapeutic implications of endothelial progenitor cells in angiogenic-mediated tumour metastasis
Publication date: Available online 18 February 2016
Source:Critical Reviews in Oncology/Hematology
Author(s): Valentina Flamini, Wen G. Jiang, Jane Lane, Yu-Xin Cui
Cancer conveys profound social and economic consequences throughout the world. Metastasis is responsible for approximately 90% of cancer-associated mortality and, when it occurs, cancer becomes almost incurable. During metastatic dissemination, cancer cells pass through a series of complex steps including the establishment of tumour-associated angiogenesis. The human endothelial progenitor cells (hEPCs) are a cell population derived from the bone marrow which are required for endothelial tubulogenesis and neovascularization. They also express abundant inflammatory cytokines and paracrine angiogenic factors. Clinically hEPCs are highly correlated with relapse, disease progression, metastasis and treatment response in malignancies such as breast cancer, ovarian cancer and non-small-cell lung carcinoma. It has become evident that the hEPCs are involved in the angiogenesis-required progression and metastasis of tumours. However, it is not clear in what way the signalling pathways, controlling the normal cellular function of human BM-derived EPCs, are hijacked by aggressive tumour cells to facilitate tumour metastasis. In addition, the actual roles of hEPCs in tumour angiogenesis-mediated metastasis are not well characterised. In this paper we reviewed the clinical relevance of the hEPCs with cancer diagnosis, progression and prognosis. We further summarised the effects of tumour microenvironment on the hEPCs and underlying mechanisms. We also hypothesized the roles of altered hEPCs in tumour angiogenesis and metastasis. We hope this review may enhance our understanding of the interaction between hEPCs and tumour cells thus aiding the development of cellular-targeted anti-tumour therapies.
from #MedicinebyAlexandrosSfakianakis via xlomafota13 on Inoreader http://ift.tt/20Gacdq
via IFTTT
Wear Behavior of Aluminium Metal Matrix Composite Prepared from Industrial Waste
With an increase in the population and industrialization, a lot of valuable natural resources are depleted to prepare and manufacture products. However industrialization on the other hand has waste disposal issues, causing dust and environmental pollution. In this work, Aluminium Metal Matrix Composite is prepared by reinforcing 10 wt% and 20 wt% of wet grinder stone dust particles an industrial waste obtained during processing of quarry rocks which are available in nature. In the composite materials design wear is a very important criterion requiring consideration which ensures the materials reliability in applications where they come in contact with the environment and other surfaces. Dry sliding wear test was carried out using pin-on-disc apparatus on the prepared composites. The results reveal that increasing the reinforcement content from 10 wt% to 20 wt% increases the resistance to wear rate.
from #MedicinebyAlexandrosSfakianakis via xlomafota13 on Inoreader http://ift.tt/1otmla7
via IFTTT
Insertion of CO2 into the Carbon-Boron Bond of a Boronic Ester Ligand
Chem. Commun., 2016, Accepted Manuscript
DOI: 10.1039/C6CC01257D, Communication
DOI: 10.1039/C6CC01257D, Communication
Trevor Janes, Kimberly M. Osten, Adam Pantaleo, Ellen Yan, Yanxin Yang, Datong Song
New Ru and Zn diazafluorenyl complexes undergo C-H borylation of the diazafluorenyl ligand to form the corresponding diazafluorenylboronic ester complexes, which can insert CO2 into their C-B bonds to form...
The content of this RSS Feed (c) The Royal Society of Chemistry
New Ru and Zn diazafluorenyl complexes undergo C-H borylation of the diazafluorenyl ligand to form the corresponding diazafluorenylboronic ester complexes, which can insert CO2 into their C-B bonds to form...
The content of this RSS Feed (c) The Royal Society of Chemistry
from #MedicinebyAlexandrosSfakianakis via xlomafota13 on Inoreader http://ift.tt/210Pnil
via IFTTT
Neuregulin 1 confers neuroprotection in SOD1-linked amyotrophic lateral sclerosis mice via restoration of C-boutons of spinal motor neurons
Abstract
Introduction
Increasing evidence implicates the role of the cell types surrounding motor neurons, such as interneurons and glial cells, in non-cell autonomous neurodegeneration of amyotrophic lateral sclerosis (ALS). C-boutons, the large cholinergic synapses that innervate spinal α-motor neurons to control their excitability, are progressively lost from motor neurons in both human ALS and mutant Cu/Zn superoxide dismutase 1 (SOD1)-ALS mice. Neuregulin-1 (NRG1), a trophic factor implicated in neural development, transmission, and synaptic plasticity, has been reported to localize in the synapse of C-boutons. However, the roles of NRG1 in maintenance of motor neuron health and activity, as well as the functional consequences of its alteration in motor neuron disease, are not fully understood.
Results
NRG1 was localized to the post-synaptic face of C-boutons and its expression was significantly lost in SOD1-ALS mice and human ALS patients. Losses of NRG1 expression and C-boutons occured almost contemporaneously in SOD1-ALS mice. In addition, expressions of ErbB3 and ErbB4, receptors for NRG1, were reduced in the motor neurons of SOD1-ALS mice. Furthermore, viral-mediated delivery of type III-NRG1 to the spinal cord restored the number of C-boutons and extended the survival time of SOD1-ALS mice.
Conclusions
These results suggest that maintenance of NRG1-ErbB4/3 axis by supplementation of NRG1 confers neuroprotection in motor neuron disease, partly through the maintenance of C-boutons of spinal motor neurons.
from #MedicinebyAlexandrosSfakianakis via xlomafota13 on Inoreader http://ift.tt/1QnU9Lu
via IFTTT
Toll-like receptor 5 and 7 expression in adenoid cystic carcinoma of major salivary glands
Abstract
Adenoid cystic carcinoma (ACC) of the salivary glands has a poor long-term prognosis and high metastatic rate. Toll-like receptors (TLRs) have been related to tumour progression but have also tumour growth-inhibiting responses. To the best of our knowledge, they have not been studied previously in ACC. We studied the immunoexpression of TLR 5 and 7 in ACC of the major salivary glands. From a cohort of 54 patients with ACC of the major salivary glands treated at the Department of Otorhinolaryngology-Head and Neck Surgery, Helsinki University Hospital, Helsinki, Finland in 1974–2009, there were 34 primary tumours and six metastases available for immunohistochemical analysis. Immunohistochemical expression of TLR 5 and 7 were correlated to clinicopathological findings and patient survival. Both TLR 5 and 7 were expressed in ACCs and their metastases, mostly on the cell membranes. The expression was heterogeneous in individual tumours. TLR 5 was expressed less in male samples, and TLR 7 had lower expression in ACCs with solid growth pattern. No correlation with survival was found. In the normal salivary gland, the TLR 5 and 7 expression was mainly negative. Both TLR 5 and 7 are expressed in salivary adenoid cystic carcinoma on the cell membranes as well as in cytoplasm.
from #MedicinebyAlexandrosSfakianakis via xlomafota13 on Inoreader http://ift.tt/1otkVfF
via IFTTT
Low temperature plasmas as emerging cancer therapeutics: the state of play and thoughts for the future
Abstract
The field of plasma medicine has seen substantial advances over the last decade, with applications developed for bacterial sterilisation, wound healing and cancer treatment. Low temperature plasmas (LTPs) are particularly suited for medical purposes since they are operated in the laboratory at atmospheric pressure and room temperature, providing a rich source of reactive oxygen and nitrogen species (RONS). A great deal of research has been conducted into the role of reactive species in both the growth and treatment of cancer, where long-established radio- and chemo-therapies exploit their ability to induce potent cytopathic effects. In addition to producing a plethora of RONS, LTPs can also create strong electroporative fields. From an application perspective, it has been shown that LTPs can be applied precisely to a small target area. On this basis, LTPs have been proposed as a promising future strategy to accurately and effectively control and eradicate tumours. This review aims to evaluate the current state of the literature in the field of plasma oncology and highlight the potential for the use of LTPs in combination therapy. We also present novel data on the effect of LTPs on cancer stem cells, and speculatively outline how LTPs could circumvent treatment resistance encountered with existing therapeutics.
from #MedicinebyAlexandrosSfakianakis via xlomafota13 on Inoreader http://ift.tt/1PT4pMx
via IFTTT
Peripheral activities of growth hormone-releasing hormone
Abstract
Growth hormone (GH)-releasing hormone (GHRH) is produced by the hypothalamus and stimulates GH synthesis and release in the anterior pituitary gland. In addition to its endocrine role, GHRH exerts a wide range of extrapituitary effects which include stimulation of cell proliferation, survival and differentiation, and inhibition of apoptosis. Accordingly, expression of GHRH, as well as the receptor GHRH-R and its splice variants, has been demonstrated in different peripheral tissues and cell types. Among the direct peripheral activities, GHRH regulates pancreatic islet and β-cell survival and function and endometrial cell proliferation, promotes cardioprotection and wound healing, influences the immune and reproductive systems, reduces inflammation, indirectly increases lifespan and adiposity and acts on skeletal muscle cells to inhibit cell death and atrophy. Therefore, it is becoming increasingly clear that GHRH exerts important extrapituitary functions, suggesting potential therapeutic use of the peptide and its analogs in a wide range of medical settings.
from #MedicinebyAlexandrosSfakianakis via xlomafota13 on Inoreader http://ift.tt/1mJGtTF
via IFTTT
Intracochlear Position of Cochlear Implants Determined Using CT Scanning versus Fitting Levels: Higher Threshold Levels at Basal Turn
Objectives: In this study, the effects of the intracochlear position of cochlear implants on the clinical fitting levels were analyzed. Design: A total of 130 adult subjects who used a CII/HiRes 90K cochlear implant with a HiFocus 1/1J electrode were included in the study. The insertion angle and the distance to the modiolus of each electrode contact were determined using high-resolution CT scanning. The threshold levels (T-levels) and maximum comfort levels (M-levels) at 1 year of follow-up were determined. The degree of speech perception of the subjects was evaluated during routine clinical follow-up. Results: The depths of insertion of all the electrode contacts were determined. The distance to the modiolus was significantly smaller at the basal and apical cochlear parts compared with that at the middle of the cochlea (p Conclusions: The stimulation levels of the cochlear implants were affected by the intracochlear position of the electrode contacts, which were determined using postoperative CT scanning. Interestingly, these levels depended on the insertion depth, whereas the distance to the modiolus did not affect the stimulation levels. The T-levels increased toward the basal end of the cochlea. The level profiles were independent of the overall stimulation levels and were not affected by the biographical data of the patients, such as the duration of deafness, age at implantation or time since implantation. Further research is required to elucidate how fitting using level profiles with an increase toward the basal end of the cochlea benefits speech perception. Future investigations may elucidate an explanation for the effects of the intracochlear electrode position on the stimulation levels and might facilitate future improvements in electrode design.
Audiol Neurotol 2016;21:54-67
from #MedicinebyAlexandrosSfakianakis via xlomafota13 on Inoreader http://ift.tt/1Ks0gT1
via IFTTT
Audiol Neurotol 2016;21:54-67
from #MedicinebyAlexandrosSfakianakis via xlomafota13 on Inoreader http://ift.tt/1Ks0gT1
via IFTTT
"Zhonghua Yi Xue Za Zhi"[jour]; +29 new citations
29 new pubmed citations were retrieved for your search. Click on the search hyperlink below to display the complete search results:
"Zhonghua Yi Xue Za Zhi"[jour]
These pubmed results were generated on 2016/02/18
PubMed comprises more than 24 million citations for biomedical literature from MEDLINE, life science journals, and online books. Citations may include links to full-text content from PubMed Central and publisher web sites.
from #MedicinebyAlexandrosSfakianakis via xlomafota13 on Inoreader http://ift.tt/1mJGtD4
via IFTTT
1471 delTTCT a Common Mutation of Tunisian Patients with Lysinuric Protein Intolerance.
1471 delTTCT a Common Mutation of Tunisian Patients with Lysinuric Protein Intolerance.
Clin Lab. 2015;61(12):1973-7
Authors: Esseghir N, Bouchlaka CS, Fredj SH, Ben Chehida A, Azzouz H, Fontaine M, Tebib N, Briand G, Messaoud T, Elgaaied AB, Kaabachi N
Abstract
BACKGROUND: Lysinuric protein intolerance is an inherited aminoaciduria caused by defective cationic amino acid transport. It is an autosomal recessive disease caused by mutations in the SLC7A 7 gene. The objective of this study was to identify the mutations of Tunisians patients in order to offer the genetic counseling and the prenatal diagnosis to families.
METHODS: Five affected Tunisian children (4 girls and 1 boy) belonging to four consanguineous families were considered. The diagnosis was made based on the plasma for amino acids quantification by Ion Exchange chromatography, the DNA for mutational analysis by DHPLC and sequencing, and the amniotic fluid for prenatal diagnosis.
RESULTS: For the 5 patients, clinical features were dominated by failure to thrive, bone marrow abnormalities, hepatosplenomegaly, and mental retardation. The diagnosis for all patients was confirmed by biochemical analysis with hyperammonemia, hyperexcretion of urinary dibasic amino acids, and a high amount of orotic acid in the urine. The 1471 delTTCT mutation was identified in exon 9 in the homozygous state for all Tunisian patients. Genetic counseling was performed for three out of four families, four heterozygous and two homozygous healthy siblings were identified. The result of prenatal diagnosis showed the presence of the 1471 de1TTCT mutation in the homozygous state for the third pregnancy and heterozygous state for the fourth.
CONCLUSIONS: The 1471 deITTCT mutation seems to be a common mutation of Tunisian population. The identification of this specific mutation provides a tool for confirmatory diagnosis, genetic counseling, and prenatal diagnosis.
PMID: 26882824 [PubMed - in process]
from #MedicinebyAlexandrosSfakianakis via xlomafota13 on Inoreader http://ift.tt/1otloPb
via IFTTT
Molecular Characterization of a Novel Missense Mutation (Asp538Asn) in a Chinese Patient with Factor XII Deficiency.
Molecular Characterization of a Novel Missense Mutation (Asp538Asn) in a Chinese Patient with Factor XII Deficiency.
Clin Lab. 2015;61(12):1967-71
Authors: Li M, Xie H, Wang M, Ding H
Abstract
BACKGROUND: Congenital factor XII (FXH) deficiency is an autosomal recessive disorder whose genetic basis has been described in a relatively small number of cases.
METHODS: Recently, we studied a Chinese family in which the proband had obviously prolonged activated partial thromboplastin time (APTT) associated with low functional and antigen FXII levels, 5% and 6.8%, respectively. To investigate the molecular defects in this FXII-deficient patient, we performed FXII mutation screening and invitro expression studies.
RESULTS: Sequence analysis of the FXII gene revealed a heterozygous G>A transition at nucleotide 8597 in exon 13, causing a novel Asp538Asn mutation in the catalytic domain.
CONCLUSIONS: From the results above, we reasoned that this mutation must confer a cross-reacting material (CRM) negative phenotype. Additional expression studies in COS-7 cells showed that the antigen level of mutant FXII (FXII-Asp538Asn) was lower compared to the wild type in culture media, whereas the corresponding level of FXII antigen in cell lysates was equivalent roughly to that of the wild type. These findings indicated that the Asp538Asn mutation results in intracellular degradation of the mutant FXII and causes FXII deficiency.
PMID: 26882823 [PubMed - in process]
from #MedicinebyAlexandrosSfakianakis via xlomafota13 on Inoreader http://ift.tt/1mJGqHl
via IFTTT
Sustained Appearance of Urinary Podocytes Suggests Poor Renal Prognosis in Kidney Transplant Patients with Focal Segmental Glomerulosclerosis: Case Reports and Review of Literature.
Sustained Appearance of Urinary Podocytes Suggests Poor Renal Prognosis in Kidney Transplant Patients with Focal Segmental Glomerulosclerosis: Case Reports and Review of Literature.
Clin Lab. 2015;61(12):1961-6
Authors: Yokoyama C, Usui J, Kobayashi M, Hiwatashi A, Hagiwara M, Okubo R, Kai H, Nanmoku T, Kawakami Y, Nagata M, Hara M, Yamagata K
Abstract
Recent studies have indicated that the detection of urinary podocytes holds major significance for focal segmental glomerulosclerosis (FSGS). We present two cases of FSGS after kidney transplantation, focusing on urinary podocytes. In Case 1, treatment led to incomplete remission with the reduction of urinary podocytes, and his renal function was preserved. Case 2, however, showed continuous increase in proteinuria with loss of renal function despite apheresis. Urinary podocytes remained high throughout. On the basis of this experience, we suggest the significance of the detection of urinary podocytes for determining renal prognosis in FSGS following renal allograft.
PMID: 26882822 [PubMed - in process]
from #MedicinebyAlexandrosSfakianakis via xlomafota13 on Inoreader http://ift.tt/1otloyz
via IFTTT
Optimization of the Original TRIzol-Based Technique Improves the Extraction of Circulating MicroRNA from Serum Samples.
Optimization of the Original TRIzol-Based Technique Improves the Extraction of Circulating MicroRNA from Serum Samples.
Clin Lab. 2015;61(12):1953-60
Authors: Lv W, Ma W, Yin X, Chai Z, Li B, Situ B, Lin L, Wang Q, Zheng L
Abstract
BACKGROUND: MicroRNAs (miRNAs) are a group of small endogenous, non-coding RNA molecules that have been demonstrated to be essential regulators of many critical biological and pathological processes. Because of their high stability in blood and the strong implication of miRNA expression profiles for human diseases, miRNAs are currently emerging as promising circulating biomarkers for the diagnosis or prognosis of a variety of human diseases. The TRIzol-based technique has been widely used for cell or tissue RNA extraction because of its economy and reliability. However, the original TRIzol-based RNA isolation protocol was not specifically designed for microRNA extraction from serum samples. When it was used to extract serum microRNAs, due to the short sequence and low level of microRNAs, the isolation efficiency in most cases could not meet the requirement. To address this issue, in this study, an improved TRIzol-based protocol was established by modifying the extraction procedure of the original TRIzol-based protocol. The performance of the improved TRIzol-based protocol was evaluated by comparison with other methods.
METHODS: Total RNA was isolated by the improved TRIzol-based method, the original method, and two other commonly used methods. RT-qPCR and spectrophotometry were used to examine the quality and yield of total RNA.
RESULTS: The improved method was more efficient than the original protocol and more suitable for real-time PCR-based profiling experiments.
CONCLUSIONS: The optimized TRIzol-based method described in this report is suitable for the extraction of serum microRNAs and useful for the development of microRNAs as diagnostic biomarkers.
PMID: 26882821 [PubMed - in process]
from #MedicinebyAlexandrosSfakianakis via xlomafota13 on Inoreader http://ift.tt/1mJGt68
via IFTTT
A Comparison of Three Widely Used Immunoassay Systems in Cortisol Measurement.
A Comparison of Three Widely Used Immunoassay Systems in Cortisol Measurement.
Clin Lab. 2015;61(12):1947-52
Authors: Yücel N, Madenci ÖÇ, Bölük A, Köroğlu L, Temel Y, Orçun A
Abstract
BACKGROUND: Interassay variability is one of the challenging issues of routine clinical laboratory practice. Commercial plasma cortisol immunoassays are also subject to this issue. In this study, we intended to evaluate the interchangeability of cortisol results of three widely used immunoassay systems.
METHODS: The cortisol values of 150 serum samples measured by three immunoassay systems, Beckman Coulter DXI 800, Roche Modular E170, and Siemens Immulite 2500, were compared.
RESULTS: A degree of proportional biases was observed between all three methods; DXI 800 showed the worst biases with the other two systems (slope values 0.67 and 0.77 with E170 and Immulite 2500, respectively). DXI 800 showed poor agreement with other methods (CCC: 0.83 and 0.87, respectively). There was a moderate agreement between E170 and Immulite 2500 (CCC: 0.92).
CONCLUSIONS: All three methods showed a degree of variability among themselves. DXI 800 results were not interchangeable with the other two systems.
PMID: 26882820 [PubMed - in process]
from #MedicinebyAlexandrosSfakianakis via xlomafota13 on Inoreader http://ift.tt/1mJGqqP
via IFTTT
Relationship between Serum Resistin Level of Xinjiang Uygur and Han Subjects with Metabolic Syndrome.
Relationship between Serum Resistin Level of Xinjiang Uygur and Han Subjects with Metabolic Syndrome.
Clin Lab. 2015;61(12):1941-6
Authors: Han J, Yakup K, Yuan Q, Xu S, Li C, Rigeli T, Yu Y, Shen CL, Yang S
Abstract
BACKGROUND: Xinjiang is a multi-ethnic region of China. Many large population based cross-sectional studies have shown that Uygur, the largest ethnic group in the Xinjiang, had a higher incidence rate of metabolic syndrome (MetS) than other ethnic groups living in Xinjiang region, but the pathological mechanism is not yet clear. Insulin resistance plays a critical role in the pathogenesis of MetS. Resistin is an adipocyte- and monocyte-derived cytokine that represents a link between obesity, insulin resistance, and type 2 diabetes. The aim of this study is to examine the relationship between serum resistin levels and metabolic syndrome parameters in Uygur and Han in Xinjiang.
METHODS: Subjects came from a population based cross-sectional study, a total of 465 subjects were selected for the study. Blood resistin, fasting blood sugar (FBS), fasting insulin (FINS), total cholesterol (TC), low-density lipoprotein-cholesterol (LDL-C), high-density lipoprotein-cholesterol (HDL-C), triglycerides (TG), waist circumference (WC), body mass index (BMI), systolic blood pressures (SBP), diastolic blood pressures (DBP), and the homeostasis model assessment-estimated insulin resistance (HOMA-IR) indices were measured. Dietary intake data were obtained by food frequency questionnaire method. The International Diabetes Federation (IDF) criteria were adopted to define metabolic syndrome.
RESULTS: The MetS group has higher metabolic parameters of FPG, TG, TC, DBP, SBP, WC, BMI, LDL-C, and low HDL-C than the non-MetS group, Uygurs have higher FPG and WC than Hans, and Hans have higher TC, TG, and FINS than Uygurs, even after adjusting for age, gender, and BMI, indicating ethnic differences in MetS-related parameters. There was no significant difference in serum resistin levels between the MetS and non-MetS group, as well as between the Uygur and the Han ethnic groups, but a significant positive correlation was found between serum resistin levels with FIN, HOMA, and diet fat.
CONCLUSIONS: In our study, we did not observe significant differences in serum resitin levels between the MetS and non-MetS group, regardless of ethnicity. However, serum resistin was positively associated with insulin resistance markers, suggesting that resistin may be an independent determinant for insulin resistance, or indirectly contribute to the development of MetS. Therefore the role of resistin in MetS warrants further investigation.
PMID: 26882819 [PubMed - in process]
from #MedicinebyAlexandrosSfakianakis via xlomafota13 on Inoreader http://ift.tt/1mJGkiS
via IFTTT
Is There a Link Between Changes in Levels of Hepcidin and Stroke?
Is There a Link Between Changes in Levels of Hepcidin and Stroke?
Clin Lab. 2015;61(12):1935-9
Authors: Petrova JJ, Manolov VE, Hadjidekova SP, Hadjiev EA, Bogov BI, Marinov BM, Vasilev VG, Tzatchev KN
Abstract
BACKGROUND: Iron is an essential element for the living body. It is well known that iron homeostasis disorders are important in two ways--its deficiency and its overload lead to several pathologies.
METHODS: We measured 17 patients with iron deficiency anemia (IDA); 19 with anemia of chronic diseases (ACD); 15 with ischemic stroke (IS). The results were compared to a previously selected control group. For evaluation of iron metabolism status, we measured serum iron levels, ferritin, and soluble transferrin receptors. For inflammation, serum interleukin-6 and hsCRP were measured. Serum hepcidin quantification was performed using a previously validated immunosorbent method. Ferritin was measured by an ECLIA method; serum iron on AAS; hsCRP using a nephelometric analysis.
RESULTS: We found statistically significant elevated serum hepcidin levels in patients with ACD and IS compared to the control group (p < 0.001). Patients with IDA had statistically significant lower hepcidin levels compared to the control group (p < 0.001). Serum ferritin levels in the IS group was higher compared to the control and other groups (p < 0.001). The lowest ferritin concentrations were established in the IDA group compared to the control (p < 0.001). We found a strong correlation between serum hepcidin and ferritin levels in the IS group (r = 0.583; p < 0.001).
CONCLUSIONS: Quantification of serum hepcidin levels might be used as a link for prediction of acute ischemic stroke and future therapeutic influences.
PMID: 26882818 [PubMed - in process]
from #MedicinebyAlexandrosSfakianakis via xlomafota13 on Inoreader http://ift.tt/1Ua0eBs
via IFTTT
The Assessment of Liver Reserve Function by Spectrophotometry based on Determination of Phenacetin and Paracetamol.
The Assessment of Liver Reserve Function by Spectrophotometry based on Determination of Phenacetin and Paracetamol.
Clin Lab. 2015;61(12):1927-34
Authors: Ren R, Ma Y, Ma W, Lu S
Abstract
BACKGROUND: To establish a technical system for assessing liver reserve function based on spectrophotometry by detection of phenacetin and paracetamol in blood samples.
METHODS: Taking detected contents of phenacetin and paracetamol by high performance liquid chromatography (HPLC) as standard, which was proved to be able to detect drug concentrations with high resolution and accuracy, we established a technical system based on the spectrophotometric technique to assay phenacetin and paracetamol, including the color system, the maximum absorption wavelength, the influence factors of color system, and the optimal conditions for hydrolysis. Then we verified our established system compared with that under HPLC by recovery test.
RESULTS: This study established a technical system to detect phenacetin and paracetamol in blood samples using spectrophotometry. Mainly, 3 mol/L hydrochloric acid (HCl) was added to samples for hydrolysis for 30 minutes, then, adding 0.02% 1,2-naphthoquinone-4-sulfonate (NQS), 1% cetyltrimethyl ammonium bromide (CTA) and 2% sodium hydroxide (or 3% sodium carbonate) (ratio of 1:6:1:2 or 3), and the absorbance was measured at 500 nm and 570 nm to calculate their concentrations.
CONCLUSIONS: Using an established spectrophotometric system to detect phenacetin and paracetamol in blood samples could assess liver reserve function, which was proved comparable with HPLC in resolution and repeatability.
PMID: 26882817 [PubMed - in process]
from #MedicinebyAlexandrosSfakianakis via xlomafota13 on Inoreader http://ift.tt/1mJGhnk
via IFTTT
Decreased Serum miR-503 Level in Children with Nephrotic Syndrome.
Decreased Serum miR-503 Level in Children with Nephrotic Syndrome.
Clin Lab. 2015;61(12):1917-26
Authors: Wang H, Hu Z, Chen L
Abstract
BACKGROUND: Serum microRNAs (miRNA, miR) are suggested to have great potential to serve as biomarkers. In this study, we explored the clinical role of serum miRNAs which may play key roles in childhood nephrotic syndrome (NS).
METHODS: Serum samples were obtained from 150 NS children and 109 age/gender-matched healthy controls. The levels of serum miRNAs were analyzed using the TaqMan Low Density Array and then validated with RT-qPCR assay. Pathological changes were examined in NS children with immunohistochemical analysis. The effects of miR-503 on cell proliferation and cell cycle were explored using MTT assay and flow cytometry in rat mesangial cells (RMCs). Western blot analysis was applied to determine the effect of miR-503 on cell cycle-related proteins.
RESULTS: The concentration of serum miR-503 was highly decreased in NS children compared with controls (p < 0.0001). In NS children, we found obvious enhancement of cell proliferation marker Ki-67 by immunohisto- chemistry. Moreover miR-503 mimics significantly extended the G0/G1 phase and decreased the G2/M phase in comparison with the negative control group. Overexpression of miR-503 obviously reduced the levels of cyclin E. Luciferase reporter assay revealed that cyclin E was the target of miR-503.
CONCLUSIONS: In this study, we determined that serum miR-503 was significantly decreased in NS children. MiR-503 contributes to the aberrant proliferation of RMCs by targeting cyclin E, which may represent potential diagnostic and prognostic biomarkers for idiopathic pediatric NS.
PMID: 26882816 [PubMed - in process]
from #MedicinebyAlexandrosSfakianakis via xlomafota13 on Inoreader http://ift.tt/1Ua0fVT
via IFTTT
The Evaluation of Serum Pentraxin-3 and High-Sensitivity C-Reactive Protein Levels in Patients with Acute Attack of COPD.
The Evaluation of Serum Pentraxin-3 and High-Sensitivity C-Reactive Protein Levels in Patients with Acute Attack of COPD.
Clin Lab. 2015;61(12):1911-6
Authors: Duran L, Unsal M, Yardan T, Kati C, Bedir A, Turkeli S, Ekiz M
Abstract
BACKGROUND: Acute exacerbations of chronic obstructive pulmonary disease (COPD) are characterized by the release of inflammatory mediators. The aim of this study was to compare serum levels of pentraxin 3 (PTX3) and high-sensitivity C-reactive protein (hs-CRP) in patients with acute exacerbations of COPD with those of a healthy control group.
METHODS: The study included 107 men and 19 women, with mean age of 66.5 (32 - 87) years who were diagnosed with acute COPD exacerbations and 48 healthy individuals as a control group. The serum PTX3 and hs-CRP levels were measured and pulmonary function tests were performed.
RESULTS: The mean serum level of the hs-CRP was 39.56 mg/L (10.10 - 262), and it was higher in the COPD group than in the control group (p < 0.0001). The hs-CRP levels increased in accordance with the severity of the COPD (p < 0.0001). The serum PTX3 level was 0.52 pcg/dL (0.42 - 0.56) in acute exacerbations. There was a correlation between the PTX3 levels and the pulmonary function tests, including FEV1, FVC, and FEV1/FVC (r = 0.317, p < 0.001; r = 0.385, p < 0.0001, and r = 0.248, p = 0.001, respectively).
CONCLUSIONS: The short pentraxin hs-CRP is elevated in COPD patients with acute exacerbations and correlates with the severity of the disease compared with the long pentraxin PTX3. These results support the idea that hs-CRP can be used as an earlier determinant of inflammation in COPD acute exacerbations and that PTX3 cannot be used as a marker of acute exacerbation and disease severity.
PMID: 26882815 [PubMed - in process]
from #MedicinebyAlexandrosSfakianakis via xlomafota13 on Inoreader http://ift.tt/1Ua0eBd
via IFTTT
Association of Genetic Polymorphisms in UDP-Glucuronosyltransferases 2B17 with the Risk of Pancreatic Cancer in Chinese Han Population.
Association of Genetic Polymorphisms in UDP-Glucuronosyltransferases 2B17 with the Risk of Pancreatic Cancer in Chinese Han Population.
Clin Lab. 2015;61(12):1905-10
Authors: Che X, Yu D, Wu Z, Zhang J, Chen Y, Han Y, Wang C, Qi J
Abstract
BACKGROUND: This study is aimed to investigate the association between polymorphisms in UGT2B17 and the risk of developing pancreatic cancer in Chinese Han population.
METHODS: A hospital-based case-control study was conducted, and 1579 healthy controls and 406 pancreatic cancer patients were enrolled. Real-time PCR was applied to identify the genetic polymorphisms in the subjects, and multivariable logistic regression analysis was performed to investigate the association between UGT2B17 polymorphisms and susceptibility to pancreatic cancer.
RESULTS: The prevalence of the UGT2B17 del/del, del/ins, and ins/ins in cases were 72.9%, 24.0%, and 3.1%, respectively, and in controls 66.6%, 30.7%, and 2.7%, respectively. Multivariable logistic regression revealed that, compared with the del/del genotype, the del/ins genotype in UGT2B17 is related to a significant reduction in pancreatic cancer risk (OR = 0.77, 95% CI = 0.60 - 0.99; P = 0.04). In the female subjects, compared with the del/del genotype, the del/ins genotype was related to a substantial reduction in pancreatic cancer risk (OR = 0.59, 95% CI = 0.39 - 0.90, P = 0.01).
CONCLUSIONS: All these results indicate a higher ratio of UGT2B17 deletion polymorphisms in Asians. UGT2B17 deletion polymorphisms are associated with the risk of developing pancreatic cancer in Chinese Han population, especially in the female population.
PMID: 26882814 [PubMed - in process]
from #MedicinebyAlexandrosSfakianakis via xlomafota13 on Inoreader http://ift.tt/1Ua0ekR
via IFTTT
A Single Nucleotide Polymorphism in the FOXP3 Gene Associated with Behçet's Disease in an Iranian Population.
A Single Nucleotide Polymorphism in the FOXP3 Gene Associated with Behçet's Disease in an Iranian Population.
Clin Lab. 2015;61(12):1897-903
Authors: Hosseini A, Shanehbandi D, Estiar MA, Gholizadeh S, Khabbazi A, Khodadadi H, Sakhinia E, Babaloo Z
Abstract
UNLABELLED: Background: Behçet's Disease (BD) is a rare autoimmune disease that involves the dysfunction of regulatory T cells. FOXP3 is a key transcription factor in the development and function of T(reg) cells. Recent studies have shown SNPs in the FOXP3 contribute to the susceptibility to some autoimmune disorders.
METHODS: To clarify the association between the FOXP3 gene and the risk of BD, 50 patients diagnosed with BD and 50 healthy controls from north-western Iran were genotyped by PCR-RFLP (Mun I and Pst I) for two SNPs including rs3761547 (-3499T/C) and rs3761548 (-3279 C/A) in the promoter region of the FOXP3 gene. In addition, a 506 bp nucleotide sequence of FOXP3 promoter was analyzed.
RESULTS: The allele -3279 C/A was significantly associated with BD [p = 0.002; odds ratio (OR) = 3.841; 95% confidence interval (CI) 1.610 - 9.161]; whereas, there was no contribution of the FOXP3 polymorphism -3499T/C to BD [(p = 0.084); (OR = 0.348, 95% CI = 0.101 - 1.195)]. Meanwhile, sequence analysis showed 100% similarity in both controls and BD patient groups.
CONCLUSIONS: Therefore, the SNP rs3761548 in the FOXP3 gene appears to contribute to the risk of Behçet's disease among the north-western Iranian population.
PMID: 26882813 [PubMed - in process]
from #MedicinebyAlexandrosSfakianakis via xlomafota13 on Inoreader http://ift.tt/1mJGiYr
via IFTTT
Εγγραφή σε:
Αναρτήσεις (Atom)