Development of Hf4+-immobilized polydopamine-coated magnetic graphene for highly selective enrichment of phosphopeptides

Publication date: 1 March 2016
Source:Talanta, Volume 149
Author(s): Haizhu Lin, Chunhui Deng
In this work, we first designed and synthesized an IMAC material with Hf⁴⁺ immobilized on polydopamine-coated magnetic graphene and applied it to phosphopeptides enrichment successfully. The newly prepared material gathered the advantages of large specific surface area of graphene, good hydrophilicity and biocompatibility of polydopamine and superparamagnetism of Fe3O4. We investigated the performance of Hf⁴⁺-immobilized polydopamine-coated magnetic graphene (denoted as magG@PDA-Hf⁴⁺) in phosphopeptides enrichment and the results showed high selectivity and sensitivity of the new material. Finally, we successfully applied magG@PDA-Hf⁴⁺ to phosphopeptides enrichment from non-fat milk digests and human serum, further demonstrating excellent performance of this new material in phosphopeptides enrichment.

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