Detection of Quiescent Infections with Multiple Elephant Endotheliotropic Herpesviruses EEHV2, EEHV3, EEHV6 and EEHV7 within Lymphoid Lung Nodules or Lung and Spleen Tissue Samples from Five Asymptomatic Adult African Elephants.

Detection of Quiescent Infections with Multiple Elephant Endotheliotropic Herpesviruses EEHV2, EEHV3, EEHV6 and EEHV7 within Lymphoid Lung Nodules or Lung and Spleen Tissue Samples from Five Asymptomatic Adult African Elephants.

J Virol. 2015 Dec 30;

Authors: Zong JC, Heaggans SY, Long SY, Latimer EM, Nofs SA, Bronson E, Casares M, Fouraker MD, Pearson VR, Richman LK, Hayward GS

Abstract
More than 80 cases of lethal hemorrhagic disease associated with Elephant Endotheliotropic Herpesviruses (EEHV) have been identified in young Asian elephants worldwide. Diagnostic PCR tests detected six types of EEHV in blood of acute disease cases, although EEHV1A is the predominant pathogenic type. Previously, the presence of herpesvirus virions within benign lung and skin nodules from healthy African elephants led to suggestions that African elephants may be the source of EEHV disease in Asian elephants. Here, we used direct PCR DNA sequencing to detect EEHV genomes in necropsy tissue from five healthy adult African elephants. Two large lung nodules collected from culled wild South African elephants contained high levels of either EEHV3 alone or of both EEHV2 and EEHV3. Similarly, a euthanized USA elephant proved to harbor multiple EEHV types distributed non-uniformly across four small lung nodules, including high levels of EEHV6, lower levels of EEHV3 and EEHV2, and a new GC-rich branch type EEHV7. Several of the same EEHV types were also detected in random lung and spleen samples from two other elephants. Sanger PCR DNA sequence data totaling 100-kb was obtained amongst the total of 15 different strains identified with (except for a few hypervariable genes) the EEHV2, EEHV3, EEHV6 strains all being closely related to known genotypes from acute disease cases, whereas the seven loci (4.0-kb) obtained from EEHV7 averaged 18% divergence from their nearest relative EEHV3. Overall, we conclude that these four EEHV species, but probably not EEHV1, occur commonly as quiescent infections in African elephants.
IMPORTANCE: Acute hemorrhagic disease characterized by high level viremia with members of the Proboscivirus genus threatens the future breeding success of endangered Asian elephants worldwide. Although the genomes of six EEHV types from acute cases have been partially or fully characterized, lethal disease predominantly involves a variety of strains of EEHV1 whose natural host has been unclear. Here we carried out partial PCR sequencing genotype analyses on necropsy tissue from five asymptomatic African elephants and identified multiple simultaneous infections by several different EEHV types, including high concentrations in lymphoid lung nodules. Overall, the results provide strong evidence that EEHV2, EEHV3, EEHV6 and EEHV7 represent natural ubiquitous infections in African elephants, whereas Asian elephants harbor EEHV1A, EEHV1B, EEHV4 and EEHV5. Although a single case of fatal cross-species infection by EEHV3 is known, the results do not support a previous concept that highly pathogenic EEHV1A crossed from African to Asian elephants in zoos.

PMID: 26719245 [PubMed - as supplied by publisher]

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