Quantification of adipose transfer viability using a novel, bioluminescent murine model

Publication date: Available online 23 February 2016
Source:Journal of Plastic, Reconstructive & Aesthetic Surgery
Author(s): A.A. Gassman, K.K. Kao, J.P. Bradley, J.C. Lee
Fat grafting has highly variable long-term results. Research efforts to improve the reliability of fat grafting are limited by inefficient methods to evaluate fat engraftment. In this work, we describe a novel animal model for quantitative evaluation of fat grafting using in-vivo bioluminescence of adipocytes from luciferase expressing mice. Subcutaneous adipose tissue from GFP and luciferase-expressing FVB mice were obtained. The samples were homogenized, decanted, and injected into the dorsal skin folds of wildtype FVB mice. Viability of the transferred tissue was examined over a 28-day time period with quantitative bioluminescence after luciferin injection. All animals demonstrated viable adipose transfer with bioluminescence detectable on days 0, 1, 7, 14, and 28. This animal model may be used for non-invasive, longitudinal studies, to quantify the fat engraftment process.



from #MedicinebyAlexandrosSfakianakis via xlomafota13 on Inoreader http://ift.tt/1LFuD3f
via IFTTT