Cerium Oxide-Incorporated Calcium Silicate Coating Protects MC3T3-E1 Osteoblastic Cells from H 2 O 2 -Induced Oxidative Stress

Abstract

Oxidative stress regulates cellular functions in multiple pathological conditions, including bone formation by osteoblastic cells. In this work, the protective effects of cerium oxide (CeO₂)-incorporated calcium silicate (CeO₂-CS) coating on the response of osteoblasts to H₂O₂-induced oxidative stress and the related mechanism were examined. CeO₂ incorporation significantly improved osteoblast viability and reduced cell apoptosis caused by H₂O₂ when compared with the control. H₂O₂-induced reduction of differentiation marker alkaline phosphatase (ALP) was recovered in the presence of the CeO₂-CS coating. The above effects were mediated by the antioxidant effect of CeO₂. The CeO₂-CS coating immersed in 0.1 mM H₂O₂ aqueous solution was able to degrade 64 % of it in 1 week. In addition, CeO₂ incorporation decreased reactive oxygen species (ROS) production and suppressed malondialdehyde (MDA) formation in H₂O₂-treated osteoblasts. Taken together, CeO₂-CS biomedical coatings with antioxidant property would be promising for bone regeneration under oxidative stress.

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