Targeted silencing of survivin in cancer cells by siRNA loaded chitosan magnetic nanoparticles.

Targeted silencing of survivin in cancer cells by siRNA loaded chitosan magnetic nanoparticles.

Expert Rev Anticancer Ther. 2016 Apr 29;

Authors: Unsoy G, Gunduz U

Abstract
OBJECTIVE: The aim of this study was to silence Survivin expression, related with drug-resistance, via siRNA-loaded CS-MNPs.
METHODS AND RESULTS: The highest siRNA-loading efficiency was achieved at siRNA:CS-MNP ratio of 1:2. Nanoparticles had spherical morphology and homogenous size distribution in TEM. After siRNA loading, core sizes (3-5nm) of CS-MNPs didn't change significantly, however hydrodynamic diameters increased ~10nm, indicating swelling of chitosan coat due to efficient siRNA loading. 73% of siRNA was pH-dependently released at 24hrs, after 30% burst release at first 3.5hrs. Stability was high enough to keep siRNAs in CS-MNPs at pH7.2. Cellular-internalization of Survivin-siRNA-CS-MNPs was high and localized in cytoplasm of cells.
CONCLUSION: Although, mock-siRNA loaded/unloaded CS-MNPs weren't cytotoxic, cell-death of breast cancer cells was significantly increased, after the treatment of Survivin-siRNA-loaded CS-MNPs. This reveals, successful loading of Survivin-siRNA on CS-MNPs and significant silencing of Survivin expression by triggering cell-death. Consequently, CS-MNPs are highly efficient delivery systems for intact siRNAs.

PMID: 27130312 [PubMed - as supplied by publisher]

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