BREAKDOWN IN PERIPHERAL IMMUNE TOLERANCE IN EXPERIMENTAL DIABETES MELLITUS

2016-06-26T18-31-42Z
Source: Journal of Molecular Pathophysiology
Alex Kamyshny, Denis Putilin, Vita Kamyshna.
BREAKDOWN IN PERIPHERAL IMMUNE TOLERANCE IN EXPERIMENTAL DIABETES MELLITUS A.M.Kamyshnyi, D.A.Putilin, V.A.Kamyshna Zaporozhye State Medical University Abstract: We studied to determine the effect of the levels of Deaf1 mRNA expression on the nature of Foxp3+ Treg cells differentiation during experimental STZ-induced diabetes mellitus (EDM) in rats PLN. To determine the level of Deaf1 mRNA expression was performed RT-PCR in real-time by thermocycler CFX96 Real-Time PCR Detection Systems. The Foxp3+-immunopositive lymphocytes were determined using an indirect immunofluorescence technique with using a monoclonal rat antibody. We established that development of EDM was accompanied by decreased the expression levels of the transcriptional regulator Deaf1 4,2‐fold in rats PLN with 3-week EDM and 2,5‐fold in rats with 5-week EDM. Reduced Deaf1 mRNA expression during EDM associated with an decreased of total amount of Treg in the PLN, led to changes of distribution into individual classes of FoxP3+ lymphocytes and FoxP3 concentration in immunopositive cells. Key words: experimental diabetes mellitus; deformed autoregulatory factor 1 (Deaf1); Foxp3; Treg.


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