Escherichia coli probiotic strain ED1a in pigs has a limited impact on the gut carriage of extended-spectrum beta-lactamase-producing E. coli [PublishAheadOfPrint]

Four trials were conducted to evaluate the impact of Escherichia coli probiotic strain ED1a (EcED1a) administration to pigs on the gut carriage or survival in manure of extended-spectrum beta-lactamase-producing E. coli. Groups of pigs were orally inoculated with strain E. coli M63 (EcM63) carrying the bla_CTX-M-1 gene (n=84) or used as control (n=26). In the first two trials, eight days later, 24 of 40 EcM63 inoculated pigs were given EcED1a orally for six days. In the third trial, 10 EcM63 inoculated pigs were given either EcED1a or probiotic E. coli Nissle 1917 (EcN) for five days. In the fourth trial, EcED1a was given to a sow and its 12 piglets, the latter, as well as 12 piglets which did not received EcED1a, then being inoculated with EcM63. Fecal shedding of cefotaxime-resistant Enterobacteriaceae (CTX-RE) was studied by culture and bla_CTX-M-1 genes were quantified by PCR. The persistence of CTX-RE in manure samples from inoculated pigs, or inoculated in vitro with EcM63 with or without probiotics was studied. Results showed that EcM63and EcED1a were good gut colonizers. The reduction in fecal excretion of CTX-RE in EcED1a-treated pigs compared to non-treated pigs was usually inferior to 1 log10 CFU and mainly observed during the probiotic administration period. The results obtained with EcN did not differ significantly from those with EcED1a. CTX-RE survival did not differ significantly in manures with or without probiotics. In conclusion, in our experimental conditions, EcED1a and EcN could not durably prevent CTX-RE colonization of the pig gut.

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