pH-sensitive vibrational probe reveals a cytoplasmic protonated cluster in bacteriorhodopsin [Biophysics and Computational Biology]

Infrared spectroscopy has been used in the past to probe the dynamics of internal proton transfer reactions taking place during the functional mechanism of proteins but has remained mostly silent to protonation changes in the aqueous medium. Here, by selectively monitoring vibrational changes of buffer molecules with a temporal resolution...

http://ift.tt/2yYwhwY