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Δευτέρα 8 Ιανουαρίου 2018

Enzymatically cleavable traceless biotin tag for protein PEGylation and purification

Chem. Commun., 2018, Accepted Manuscript
DOI: 10.1039/C7CC05814D, Communication
Adam Marcin Wawro, Yusuke Aoki, Takahiro Muraoka, Kouhei Tsumoto, Kazushi Kinbara
Here we report an example of protein-PEG conjugate with a biotin tag cleavable by lipase-catalyzed hydrolysis. Very mild cleavage conditions, heterogeneous, easily separable catalyst, and traceless design make this method...
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