Accuracy of a TaqMan‐based real‐time polymerase chain reaction combined to a Novy‐MacNeal‐Nicolle medium culture for the diagnosis of American tegumentary leishmaniasis

Abstract

Dear editor, tests used for the diagnosis of tegumentary leishmaniasis (TL) have variable accuracy and in classic parasitological methods, sensitivity tends to vary according to examiner‐dependent factors¹. In vitro culture is a highly specific method but its sensitivity is limited^1,2. Real‐time PCR (qPCR) is considered a very efficient technique and it adds quantitative results to clinical analyses^3‐5. We aimed to test the accuracy of the combination of a Novy‐MacNeal‐Nicolle (NNN) medium culture and TaqMan‐based qPCR analysis for the diagnosis of TL.

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