In this issue

https://ift.tt/2IXo1q8

Vocal Cord Lateralization in Bilateral Abductor Paralysis by Extra-Endo Laryngeal Suture Technique: A Case Series

Abstract

Bilateral Abductor vocal cord paralysis is certainly amongst the most emergent situation an otorhinolaryngologist come across during his/her practice. The treatment of this condition has undergone prominent shift from a blind life-saving "Tracheostomy" to more exquisite and promising laryngeal function preserving surgeries. The objective of the study is to throw light on our experience of a novel method of lateralization of vocal cord in six patients. The procedure was found to be reliable as well as associated with numerous advantages including shorter operating time and lesser intra operative manipulation on larynx. The method is less irritant (knot on outer aspect of larynx), adjustable (knot can be tightened and loosened accordingly) and reversible (knot can be removed if patient recovers). Additional benefits of the practice are smooth learning curve and economic in nature. The procedure has also been modified from quadruple prick to triple prick technique by us. This development further led to decreased operative time as well as lowered risk of trauma to larynx.

https://ift.tt/2GDzjuk

Vocal Cord Lateralization in Bilateral Abductor Paralysis by Extra-Endo Laryngeal Suture Technique: A Case Series

Abstract

Bilateral Abductor vocal cord paralysis is certainly amongst the most emergent situation an otorhinolaryngologist come across during his/her practice. The treatment of this condition has undergone prominent shift from a blind life-saving "Tracheostomy" to more exquisite and promising laryngeal function preserving surgeries. The objective of the study is to throw light on our experience of a novel method of lateralization of vocal cord in six patients. The procedure was found to be reliable as well as associated with numerous advantages including shorter operating time and lesser intra operative manipulation on larynx. The method is less irritant (knot on outer aspect of larynx), adjustable (knot can be tightened and loosened accordingly) and reversible (knot can be removed if patient recovers). Additional benefits of the practice are smooth learning curve and economic in nature. The procedure has also been modified from quadruple prick to triple prick technique by us. This development further led to decreased operative time as well as lowered risk of trauma to larynx.

https://ift.tt/2GDzjuk

Microparticle Release from Cell Lines and Its Anti-influenza Activity

Viral Immunology, Ahead of Print.


https://ift.tt/2IH3hzh

Rethinking the prescription of biotin for dermatologic conditions

Dermatologic Therapy, EarlyView.


https://ift.tt/2IAzpZA

Carbapenem-non-Susceptible Haemophilus influenzae with Penicillin-Binding Protein 3 containing Amino Acid Insertion [PublishAheadOfPrint]

Prevalence of β-lactamase-negative ampicillin-resistant Haemophilus influenzae (BLNAR) has become a clinical concern. In BLNAR, amino acid substitutions in penicillin-binding protein 3 (PBP3) are relevant to the β-lactam resistance. Carbapenem-non-susceptible H. influenzae isolates have been rarely reported. Through antimicrobial susceptibility testing and nucleotide sequence analysis of ftsI, encoding PBP3, and utilizing a collection of H. influenzae clinical isolates in our laboratory, we obtained a carbapenem-non-susceptible clinical isolate (NUBL1772) that possessed an altered PBP3 containing V525_N526insM. The aim of this study was to reveal the effect of altered PBP3 containing V525_N526insM on reduced carbapenem susceptibility. After generating recombinant strains with altered ftsI, we performed antimicrobial susceptibility testing and competitive binding assays with fluorescent penicillin (Bocillin FL) and carbapenems. Elevated carbapenem MICs were found for the recombinant strain harboring the entire ftsI gene of NUBL1772. The recombinant PBP3 of NUBL1772 also exhibited reduced binding to carbapenem. These results demonstrate that altered PBP3 containing V525_N526insM influences reduced carbapenem susceptibility. The revertant mutant lacking the V525_N526insM exhibited lower MICs of carbapenem than NUBL1772, suggesting that this insertion affects reduced carbapenem susceptibility. MICs of β-lactam for NUBL1772 was higher than those for the recombinant possessing ftsI of NUBL1772. NUBL1772 harbored AcrR with early termination, resulting in low-level transcription of acrB and high efflux pump activity. These findings suggest that the disruption of AcrR also contributes to the reduced carbapenem susceptibility found in NUBL1772. Our results provide the first evidence that the altered PBP3 containing V525_N526insM is responsible for reduced susceptibility to carbapenem in H. influenzae.

https://ift.tt/2IEG14Y

Molecular Analysis of a blaIMP-1-Harboring Class 3 Integron in Multidrug-Resistant Pseudomonas fulva [PublishAheadOfPrint]

A multidrug-resistant (MDR) Pseudomonas fulva was isolated in 2006 from a urine sample. The isolate harbored the bla_IMP-1 gene, which was located in a chromosomal Tn402-like class 3 integron as a gene cassette array of aacA31-fosE-bla_IMP-1. Two mutations in gyrA and one mutation in parC were detected in quinolone resistance-determining regions (QRDRs). We report a full-length, novel bla_IMP-1-carrying class 3 integron. This integron and together with mutations in QRDRs could have influenced the MDR phenotypes.

https://ift.tt/2IA5JvD