Identification of cypermethrin induced proteins changes in green algae by iTRAQ quantitative proteomics

Publication date: Available online 4 March 2016
Source:Journal of Proteomics
Author(s): Yan Gao, Teck Kwang Lim, Qingsong Lin, Sam Fong Yau Li
Cypermethrin (CYP) is one of the most widely used pesticides in large scale for agricultural and domestic purpose and the residue often seriously affects aquatic system. Environmental pollutants induced protein changes in organisms could be detected by proteomics, leading to discovery of potential biomarkers and understanding of mode of action. While proteomics investigations of CYP stress in some animal models have been well studied, few reports about the effects of exposure to CYP on algae proteome were published. To determine CYP effect in algae, the impact of various dosages (0.001μg/L, 0.01μg/L and 1μg/L) of CYP on green algae Chlorella vulgaris for 24h and 96h were investigated by using iTRAQ quantitative proteomics technique. A total of 162 and 198 proteins were significantly altered after CYP exposure for 24h and 96h, respectively. Overview of iTRAQ results indicated that the influence of CYP on algae protein might be dosage-dependent. Functional analysis of differentially expressed proteins showed that CYP could induce protein alterations related to photosynthesis, stress responses and carbohydrates metabolism. This study provides a comprehensive view of complex mode of action of algae under CYP stress and highlights several potential biomarkers for further investigation of pesticides exposed plant and algae.Biological SignificanceThe effect of cypermethrin on algae proteome in term of exposure concentrations and durations has been determined using quantitative iTRAQ technique. The results showed that cypermethrin affects proteins related with photosynthesis, stress responsive and carbohydrate metabolism. Overall, this study provides comprehensive analysis of green algae protein profiles in response to cypermethrin exposure and presents some new information about plant and algae exposure to pesticide.

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