Molecular identification of miR-145 and miR-9 expression level as prognostic biomarkers for early stage cervical cancer detection.

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Molecular identification of miR-145 and miR-9 expression level as prognostic biomarkers for early stage cervical cancer detection.

QJM. 2016 Jun 26;

Authors: Azizmohammadi S, Safari A, Azizmohammadi S, Kaghazian M, Sadrkhanlo M, Yahaghi E, Farshgar R, Seifoleslami M

Abstract
BACKGROUND: MiRNAs may act as carcinogen or tumor suppressor genes by targeting various biological molecules. Therefore, it is important to identify significant markers for prognosis, diagnosis treatment strategies of cancers.
OBJECTIVE: To evaluate the clinical importance and prognostic value of miR-9 and miR-145 in cervical cancer.
METHOD: MiRNAs expression was detected using quantitative real-time PCR in cervical cancer specimens and adjacent normal tissues.
RESULTS: MiR-9 upregulated in cervical cancer specimens than adjacent normal tissues (9.743 ± 2.172vs. 2.131 ± 1.083; p<0.05). MiR-145 was decreased in cervical cancer specimens compared to corresponding normal tissues (2.189 ± 0. 724 vs. 7.173 ± 1.558 p<0.05). In addition increased expression of miR-9 were strongly linked to lymph node metastasis (P= 0.017), and vascular invasion (P= 0.011). On the other hand, the low expression of miR-145 was related to advanced FIGO stage (P= 0.007), lymph node metastasis (P= 0.02), and vascular invasion (0.026). Kaplan-Meier survival and log-rank analysis suggested that patients with high expression of miR-9 had shorter overall survival compared with those with low expression (log-rank test P= 0.028; P<0.001). In addition, shorter overall survival time was remarkably linked to decreased expression of miR-145 (log-rank test P<0.001). Multivariate Cox proportional hazards model analysis of miR-9 and miR-145 showed that FIGO stage (P= 0.011), high expression of miR-9 and low expression of miR-145 (P=0.023; P= 0.031) were independent prognostic factors for overall survival of patients.
CONCLUSIONS: MicroRNA-145 and 9 may be as potential prognostic marker in patients suffering from cervical cancer.

PMID: 27345415 [PubMed - as supplied by publisher]

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