Chromosome-encoded broad-spectrum Ambler class A {beta}-lactamase RUB-1 from Serratia rubidaea [PublishAheadOfPrint]

Whole genome sequencing of Serratia rubidaea CIP 103234^T revealed a chromosomally-located Ambler class A β-lactamase gene. The gene was cloned, and the β-lactamase, RUB-1, was characterized. RUB-1 displayed 74% and 73 % amino acid sequence identity with GIL-1 and TEM-1 penicillinases, respectively, and its substrate profile was similar to that of these latter β-lactamases. 5' RACE analysis revealed promoter sequences highly divergent from E. coli ⁷⁰ consensus sequence. This work further illustrates the heterogeneity of ß-lactamases among Serratia spp.

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