Cloning and Expression of Novel Aminoglycoside Phosphotransferase Genes from Campylobacter and Their Role in the Resistance to Six Aminoglycosides [PublishAheadOfPrint]

Nine aph genes, including aph(2'')-Ib, Ic, Ig, If, If1, If3, Ih, aac(6')-Ie/aph(2'')-Ia, and aac(6')-Ie/aph(2'')-If2 were previously identified in Campylobacter. To measure the contribution of these alleles to aminoglycoside resistance, we cloned nine genes into the pBluescript and expressed them in E. coli DH5α. The nine aph expressed in E.coli showed varying levels of resistance to gentamicin, kanamycin and tobramycin. Three genes, aac(6'')-Ie/aph(2'')-Ia, aph2''-If1 and aph2''-Ig showed increased MICs to amikacin and five aph genes were transferrable.

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