Constitutive activation of MexT by amino acid substitutions results in MexEF-OprN overproduction in clinical isolates of Pseudomonas aeruginosa [PublishAheadOfPrint]

When overproduced, the multidrug efflux system MexEF-OprN increases resistance of Pseudomonas aeruginosa to fluoroquinolones, chloramphenicol and trimethoprim. In this work, we demonstrate that gain-of-function mutations in regulatory gene mexT result in oligomerization of LysR regulator MexT, in constitutive upregulation of the efflux pump and increased resistance in clinical isolates.

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