Αρχειοθήκη ιστολογίου

Δευτέρα 22 Οκτωβρίου 2018

THE REACTION MECHANISM OF METALLO-{beta}-LACTAMASES IS TUNED BY THE CONFORMATION OF AN ACTIVE SITE MOBILE LOOP [Mechanisms of Resistance]

Carbapenems are "last resort" β-lactam antibiotics, used to treat serious and life-threatening healthcare-associated infections caused by multidrug resistant Gram-negative bacteria. Unfortunately, the worldwide spread of genes coding for carbapenemases among these bacteria is threatening these life-saving drugs. Metallo-β-Lactamases (MβLs) are the largest family of carbapenemases. These are Zn(II)-dependent hydrolases that are active against almost all β-lactam antibiotics. Their catalytic mechanism and the features driving substrate specificity have been matter of intense debate. The active sites of MβLs are flanked by two loops, one of which, loop L3, was shown to adopt different conformations upon substrate or inhibitor binding, and thus being expected to play a role in substrate recognition. However, the sequence heterogeneity observed in this loop in different MβLs has limited the generalizations about its role. Herein we report the engineering of different loops within the scaffold of the clinically relevant carbapenemase NDM-1. We find that the loop sequence dictates its conformation in the unbound form of the enzyme, eliciting different degrees of active site exposure. However, these structural changes have a minor impact on the substrate profile. Instead, we report that the loop conformation determines the protonation rate of key reaction intermediates accumulated during the hydrolysis of different β-lactams in all MβLs. This study demonstrates the existence of a direct link between the conformation of this loop and the mechanistic features of the enzyme, bringing to light an unexplored function of active site loops on MβLs.



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