Αρχειοθήκη ιστολογίου

Κυριακή 25 Νοεμβρίου 2018

Detection and quantification of Porphyromonas gingivalis and Aggregatibacter actinomycetemcomitans in bacteremia induced by interdental brushing in periodontally healthy and periodontitis patients

Publication date: Available online 24 November 2018

Source: Archives of Oral Biology

Author(s): Nagore Ambrosio, María José Marín, Estefanía Laguna, David Herrera, Mariano Sanz, Elena Figuero

Abstract
Objective

This study was aimed to compare the presence and amounts of bacteremia induced by interdental brushing in healthy (H) and periodontitis (P) individuals using culture based (direct culture [DC]) and molecular based techniques (real-time quantitative polymerase chain reaction [qPCR]) in a cross-sectional study model.

Materials and Methods

After a full mouth periodontal evaluation, blood samples were taken before and 1 min after professionally-administered interdental tooth brushing. These samples were analyzed by DC and qPCR, targeting Porphyromonas gingivalis and Aggregatibacter actinomycetemcomitans. Subgingival samples were also collected and analyzed. Student t-test, chi-square tests correlations were used for analyzing the data.

Results

Thirty individuals per group were included. P. gingivalis and A. actinomycetemcomitans were detected with qPCR methods, but not with DC. At baseline, bacteremia was observed in 5 P patients (16.7%) and in 2 H individuals (6.6%) (p = 0.421). After interdental tooth brushing, bacteremia was only observed in 2 P patients (6.6%) (p = 0.901). A positive correlation between subgingival and blood levels of A. actinomycetemcomitans was observed (r = 0.3; p = 0.013).

Conclusion

Bacteremia related to A. actinomycetemcomitans and P. gingivalis did not significantly increase after a single session of use of interdental brushes.



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