Αρχειοθήκη ιστολογίου

Παρασκευή 2 Νοεμβρίου 2018

MMP-3 and MMP-8 in Rat Mandibular Condylar Cartilage Associated with Dietary Loading, Estrogen Level, and Aging

Publication date: Available online 1 November 2018

Source: Archives of Oral Biology

Author(s): Jia Yu, Eerika Mursu, Matleena Typpo, Sakari Laaksonen, Hanna-Marja Voipio, Paula Pesonen, Aune Raustia, Pertti Pirttiniemi

Abstract
Objectives

The structure of the mandibular condylar cartilage (MCC) is regulated by dynamic and multifactorial processes. The aim of this study was to examine the effects of altered dietary loading, estrogen level, and aging on the structure of the condylar cartilage and the expressions of matrix metalloproteinase (MMP) -3 and MMP-8 of rat MCC.

Methods

In this study, Crl:CD (SD) female rats were randomly divided into 3 groups according to dietary hardness: hard diet (diet board), normal diet (pellet), and soft diet (powder). In each group, the rats were further divided into 2 subgroups by ovariectomy at the age of 7 weeks. The rats were sacrificed at 5- and 14-month-old. Histomorphometric analysis of the MCC thickness was performed after toluidine blue staining. Immunochemical staining was done for MMP-3 and MMP-8. A linear mixed model was used to assess the effects of dietary loading, estrogen level, and aging. Results: Increased dietary loading was the main factor to increase the MMP-3 expression and the anterior and central thickness of the MCC. Lack of estrogen was the main factor associated with decreased MMP-8. Aging was associated with the thickness changes of the whole condylar cartilage and the reduced expression of MMP-8.

Conclusion

The condylar cartilage structure and metabolism of the female rats are sensitive to dietary loading changes, estrogen level as well as aging. The proper balance of these factors seems to be essential for the maintenance of the condylar cartilage.



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