Monitoring antiretroviral therapy using measurements of viral load (VL) and the genotyping of resistance mutations is not routinely performed in low- to middle-income countries because of the high costs of the commercial assays that are used. The analysis of dried plasma spot (DPS) samples on filter paper may represent an alternative for resource-limited settings. Therefore, we evaluated the usefulness of analyzing DPS samples to determine VL and identify drug resistance mutations (DRM) in a group of HIV-1 patients. The VL was measured from 22 paired plasma and DPS samples. In these samples, the average VL was 4.7 log10 copies/mL in liquid plasma and 4.1 log10 copies/mL in DPS, with a correlation coefficient of = 0.83. A 1.1 kb fragment of HIV pol could be amplified in 14/22 (63.6%) of the DPS samples and the same value was amplified in plasma samples. A collection of ten paired DPS and liquid plasma samples was evaluated for the presence of DRM; an excellent correlation was found in the identification of DRM between the paired samples. All HIV-1 pol sequences that were obtained corresponded to HIV subtype B. The analysis of DPS samples offers an attractive alternative for monitoring ARV therapy in resource-limited settings.
from #MedicinebyAlexandrosSfakianakis via xlomafota13 on Inoreader http://ift.tt/1N8JB4o
via IFTTT
Αρχειοθήκη ιστολογίου
-
►
2020
(289)
- ► Φεβρουαρίου (28)
-
►
2019
(9071)
- ► Δεκεμβρίου (19)
- ► Σεπτεμβρίου (54)
- ► Φεβρουαρίου (3642)
- ► Ιανουαρίου (3200)
-
►
2018
(39872)
- ► Δεκεμβρίου (3318)
- ► Σεπτεμβρίου (3683)
- ► Φεβρουαρίου (2693)
- ► Ιανουαρίου (3198)
-
►
2017
(41099)
- ► Δεκεμβρίου (3127)
- ► Σεπτεμβρίου (2173)
-
►
2016
(13807)
- ► Δεκεμβρίου (700)
- ► Σεπτεμβρίου (600)
- ► Φεβρουαρίου (1350)
- ► Ιανουαρίου (1400)
-
▼
2015
(1500)
-
▼
Δεκεμβρίου
(1450)
-
▼
Δεκ 08
(50)
- Television distribution: economic dimensions, emer...
- Acceptable range for structural fire resistance in...
- Analyzing the Divide between FPGA Academic and Com...
- Effects of imidacloprid in combination with λ-cyha...
- Design, construction and testing of a COC 3D flow-...
- Legal aspects of end-of-life decisions of minors: ...
- Flexibility and extracellular opening determine th...
- De betekenis van producent Jean Van Raemdonck voor...
- EHRM herinnert aan belang hoorrecht bij vechtschei...
- Chronic Myelogenous Leukemia Relapse Presenting Wi...
- Factors Affecting Visual Field Outcomes in the Idi...
- Acetazolamide-Induced Nephrolithiasis in Idiopathi...
- Safety and Tolerability of Acetazolamide in the Id...
- A 29-Year-Old Nurse With Fatigue, Anxiety, and Dep...
- The Optic Canal Size Is Associated With the Severi...
- Optic Nerve Sheath Melanoma Presenting as a Centra...
- Suprasellar Primitive Neuroectodermal Tumor in an ...
- Enhanced Optical Transmission through MacEtch-Fabr...
- The imbalance of masticatory muscle activity affec...
- Pallet to Palate: Extending Shelf Life and Safety ...
- Use of Dried Plasma Spots for HIV-1 Viral Load Det...
- Toxins, Vol. 7, Pages 5320-5321: Preface Biologica...
- Toxins, Vol. 7, Pages 5308-5319: Superantigen-Prod...
- Toxins, Vol. 7, Pages 5301-5307: Alkaloid-Containi...
- The Divided Cloak as Redemptio Militiae. Biblical ...
- Eliciting reciprocal peer tutoring groups' metacog...
- Preface
- Writing biography in Greece and Rome: narrative te...
- Ancient biography and formalities of fiction
- Social inclusion of persons with ID and challengin...
- Interview met Dr. Rini (Adriana Johanna) Wilmink: ...
- Empowering science teachers using technology-enhan...
- Effects of dietary lambda-cyhalothrin exposure on ...
- Emotionally biased cognitive processes: the weakes...
- Emotional attention during instructed and uninstru...
- The effect of oral administration of dsRNA on vira...
- Met fotografie en PCM aan de slag
- Persoonlijkheidsprofielen van Belgen die een PCM-t...
- De korte weg van F. Hoe film een participatief ond...
- Relationships between attention and interpretation...
- Attending to remember and remembering to attend in...
- Attentional guidance by emotional long-term memories
- The direct and indirect malleability of depression...
- The interplay between attention, interpretation an...
- Gold Standard or Fool’s Gold? The Efficacy of Disp...
- Arzneimittelnebenwirkungen im Alter
- Methylator phenotype in colorectal cancer: a progn...
- The Prevalence of Airway Problems in Children with...
- Factors Related to Persisting Perforations after V...
- The Validation of an Algorithm for the Management ...
-
▼
Δεκ 08
(50)
-
▼
Δεκεμβρίου
(1450)
Ετικέτες
Τρίτη 8 Δεκεμβρίου 2015
Use of Dried Plasma Spots for HIV-1 Viral Load Determination and Drug Resistance Genotyping in Mexican Patients
Εγγραφή σε:
Σχόλια ανάρτησης (Atom)
Δεν υπάρχουν σχόλια:
Δημοσίευση σχολίου