Iron labeled adipose stem cells and neovascularization in rabbit calvarial critical-sized defects.

Publication date: Available online 14 January 2016
Source:Oral Surgery, Oral Medicine, Oral Pathology and Oral Radiology
Author(s): Olli-Pekka Lappalainen, Marianne Haapea, Raisa Serpi, Siri Lehtonen, Leena Ylikontiola, Jarkko Korpi, Willy Serlo, George K.B. Sándor
ObjectiveThe aim of this study was to evaluate the presence of iron labelled adipose stem cells at the 2 week time point and vascular changes at the 2 and 6 week time points using two different types of scaffolds.Study designThis study included 22 White New Zealand adult male rabbits. In 6 rabbits full-thickness calvarial critical-sized defects were filled with autogenous adipose stem cells labelled with iron oxide seeded onto two scaffolds, namely solid bioactive glass (BAG) or porous tricalcium phosphate granules (TCP) used on reciprocal sides of the skull. Eleven rabbits were implanted with adipose stem cell seeded scaffolds without iron labeling for analysis of vascular changes. Five defects were left empty as negative control defects. The specimens were analyzed histologically at the 2 and 6 week time points.ResultsThe TCP group showed significantly more vascularity compared to BAG group. A greater number of labelled stem cells were identified in the TCP group compared to the BAG group, but the difference was not statistically significant.ConclusionsThis study reveals differences in stem cell distribution and revascularization of the calvarial defect which may be biomaterial dependent.



from #MedicinebyAlexandrosSfakianakis via xlomafota13 on Inoreader http://ift.tt/1RSymQP
via IFTTT