A novel mitochondrion-specific photoactivatable fluorescence turn-on bioprobe, named as o-TPE-ON+, has been designed and readily prepared, operating in a new photoactivatable mechanism of photocyclodehydrogenation. This bioprobe exhibits unique photoactivation behavior in cells, and is applied to super-resolution imaging of mitochondrion and its dynamic investigation in both fixed and live cells under physiological conditions without any external additives.
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