Interferon-β produced by osteocytes may negatively regulate osteoclastogenesis

Publication date: Available online 26 May 2016
Source:Journal of Oral Biosciences
Author(s): Chiyomi Hayashida-Abe
BackgroundAlthough old and disordered bones are continuously remodeled with new tissue to maintain their integrity, most bone surfaces are in a non-resorbing state. We hypothesized that osteocytes negatively regulate osteoclast differentiation and function to maintain a non-resorbing state. In the present study, we examined the role of osteocytes in regulating osteoclastogenesis.HighlightWhen osteoclast precursors were formed from bone marrow cells (BMs) using an macrophage-colony stimulating factor treatment with conditioned medium (CM) from osteocytic MLO-Y4 cells (MLO-Y4-CM), the generated cells exhibited a diminished capacity for osteoclastogenesis. This decreased capability was consistent with increased protein kinase R mRNA expression and decreased c-Fos translation. Furthermore, CM induced phosphorylation of STAT-1 in the osteoclast precursors. To confirm the effect of the MLO-Y4-CM, we prepared non-osteocytic cell-free osteocyte-enriched bone fragments (OEBFs). OEBFs, as well as MLO-Y4-CM, reduced the commitment of BMs into osteoclast precursors. These effects were partially abrogated by an anti-interferon (IFN)-β neutralizing antibody. Indeed, the MLO-Y4 cells and primary osteocytes embedded in the OEBFs expressed more IFN-β than osteoclast lineage cells.ConclusionWe found that osteocytes secrete IFN-β as an inhibitory factor of osteoclastogenesis. Our findings provide a new insight into the mechanisms underlying the osteocytic regulation of bone remodeling.



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