Abstract
Objective
To evaluate novel sealing techniques for their biocompatibility and sealing capacity of iatrogenic fetal membrane defects in a pregnant rabbit model.
Method
At day 23 of gestation (term=d31), a standardized fetoscopy was performed through a 14G cannula. The resulting fetal membrane defect was closed with either condensed collagen, collagen with fibrinogen, Tissuepatch®, Duraseal®, or a conventional collagen plug (Lyostypt®) as reference. At d30 the fetuses were harvested and full thickness fetal membrane samples were analyzed. The study consisted of two consecutive parts: (1) biocompatibility testing by fetal survival, apoptosis and infiltration of polymorphonuclear cells in the membranes; (2) the efficacy to seal fetal membrane defects.
Results
Three sealants (collagen with fibrinogen, Duraseal® or Lyostypt®) were associated with a higher fetal mortality compared to control unmanipulated littermates, and hence were excluded from further analysis. Tissuepatch® was biocompatible and amniotic fluid levels were comparable to those of control untouched littermates. Compared to the condensed collagen, Tissuepatch® was also easier in surgical handling and induced limited cell proliferation.
Conclusion
Tissuepatch® had the best biocompatibility and efficacy in sealing an iatrogenic fetal membrane defect in the pregnant rabbit compared to other readily available sealants.
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