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Τρίτη 12 Δεκεμβρίου 2017

Precision genome editing using synthesis-dependent repair of Cas9-induced DNA breaks [Genetics]

The RNA-guided DNA endonuclease Cas9 has emerged as a powerful tool for genome engineering. Cas9 creates targeted double-stranded breaks (DSBs) in the genome. Knockin of specific mutations (precision genome editing) requires homology-directed repair (HDR) of the DSB by synthetic donor DNAs containing the desired edits, but HDR has been reported...

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