Immunomodulation induced through ornithine decarboxylase DNA immunization in Balb/c mice infected with Leishmania donovani

Publication date: May 2018
Source:Molecular Immunology, Volume 97
Author(s): Akhilesh Kumar, Manas Ranjan Dikhit, Ajay Amit, Amir Zaidi, Raj Kishor Pandey, Ashish kumar Singh, Shashi S. Suman, Vahab Ali, Vidya Nand Rabi Das, Krishna Pandey, Vikas kumar, Shubhankar Kumar Singh, Shyam Narayan, Hirendra kumar Chourasia, Pradeep Das, Sanjiva Bimal
We report here a Leishmania donovani ornithine decarboxylase (Ld-ODC) gene used as a DNA vaccine against visceral leishmaniasis in a murine Balb/c mouse model. This study also evaluated the possible mechanism of action directed by this candidate. We found a Th1 immune response after immunization using an Ld-ODC DNA vaccine, with results based on the rearrangement of TCR-V-α-2, proliferation of Carboxy fluorescein Succinimidyle ester positive T cells, which were able to produce cytokines such as TNF-α, IFN-γ, IL-12 and IL-2, but not IL-4, IL-5, IL-6 and IL-10, and modulations of the STAT-1 and p38 MAP kinase signaling pathways. The results were corroborated with the reduction in the amastigote proliferation and parasite killing in spleens after infection in vitro. We conclude this study suggesting that the Ld-ODC DNA construct could be a new vaccine candidate against visceral leishmaniasis.

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