Clinical Correlation of Cytomegalovirus Infection with CMV-Specific CD8+ T Cell Immune Competence Score and Lymphocyte Subsets in Solid Organ Transplant Recipients

Introduction Control of cytomegalovirus (CMV) infection after solid organ transplantation (SOT) requires a functional immune system. We assessed the association between quantitation and function of CMV-specific CD8+ T cells and CMV infection in SOT recipients. Methods During a 10-year period, selected kidney, heart, lung, pancreas, liver and composite tissue recipients were tested for CMV-specific CD8+ T cells immune competence (CMV-CD8+), as measured by enumeration, interferon-gamma production and CD107a/b degranulation. Quantitative and functional data were used to assemble T cell immune competence (TIC) score. CMV infection was diagnosed by PCR in blood and other samples, or histopathology. Results Of 130 patients tested, 59 had CMV infection or disease. The median onset to CMV infection was 10.5 months (IQR 5.5-18.7). Gastrointestinal disease (28.8%), pneumonia (20.3%), and CMV syndrome (17%) were most common presentation. An impaired nonspecific or CMV-CD8+ TIC score was associated with tissue-invasive disease (HR 2.84, 95% CI 1.03-11.81; p=.04). Patients with impaired CMV-CD8+ TIC score had longer viremia duration (42.4 vs. 18.8 days, p<.001 patients with impaired nonspecific or cmv-cd8 tic score had higher risk of relapse vs. hr ci p="0.03)." cmv infection disease lower median absolute lymphocyte count cells and cd4 t cell conclusion cmv-specific cd8 function correlated the course after sot measuring these has potential to assist in its clinical management. correspondence: roshini s. abraham ph.d. department laboratory medicine pathology mayo clinic rochester mn abraham.rochini raymund r. razonable m.d. division infectious diseases razonable.raymund authorship page atibordee meesing: participation performance research data collection analysis writing manuscript abraham: reviewing razonable: design approving disclosures: none funding: copyright wolters kluwer health inc. all rights reserved.>

https://ift.tt/2ANwYQ0