Indirect immunofluorescence in mucous membrane pemphigoid: which substrate should be used?

Abstract

detection of circulating autoantibodies in mucous membrane pemphigoid (MMP) is challenging. Kamaguchi et al showed that using normal human oral mucosa (NHOM) as a substrate for indirect immunofluorescence (IIF) instead of normal human skin (NHS) or salt‐split‐skin (SSS) resulted in increased sensitivity.¹ We recently conducted a similar study in our department.

We collected serum samples from 22 patients with oral MMP. Diagnosis was made upon histopathology consistent with a subepithelial blistering process and direct immunofluorescence (DIF) showing linear IgG, IgA and/or C3 deposits at the basement membrane zone (BMZ). Serum levels of anti‐BP180 NC16A and BP230 autoantibodies were assessed by Enzyme‐Linked‐Immunosorbent‐Assay. IIF was performed on monkey oesophagus (MO), on SSS and NHOM as previously described.

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