Ribosome-inactivating proteins (RIPs) are widespread among higher plants of different taxonomic orders. In this study, we report on the RIP sequences found in the genome/transcriptome of several important Rosaceae species, including many economically important edible fruits such as apple, pear, peach, apricot, and strawberry. All RIP domains from Rosaceae share high sequence similarity with conserved residues in the catalytic site and the carbohydrate binding sites. The genomes of Malus domestica and Pyrus communis contain both type 1 and type 2 RIP sequences, whereas for Prunus mume, Prunus persica, Pyrus bretschneideri, and Pyrus communis a complex set of type 1 RIP sequences was retrieved. Heterologous expression and purification of the type 1 as well as the type 2 RIP from apple allowed to characterize the biological activity of the proteins. Both RIPs from Malus domestica can inhibit protein synthesis. Furthermore, molecular modelling suggests that RIPs from Rosaceae possess three-dimensional structures that are highly similar to the model proteins and can bind to RIP substrates. Screening of the recombinant type 2 RIP from apple on a glycan array revealed that this type 2 RIP interacts with terminal sialic acid residues. Our data suggest that the RIPs from Rosaceae are biologically active proteins.
http://ift.tt/2tMjxeC
Αρχειοθήκη ιστολογίου
-
►
2020
(289)
- ► Φεβρουαρίου (28)
-
►
2019
(9071)
- ► Δεκεμβρίου (19)
- ► Σεπτεμβρίου (54)
- ► Φεβρουαρίου (3642)
- ► Ιανουαρίου (3200)
-
►
2018
(39872)
- ► Δεκεμβρίου (3318)
- ► Σεπτεμβρίου (3683)
- ► Φεβρουαρίου (2693)
- ► Ιανουαρίου (3198)
-
▼
2017
(41099)
- ► Δεκεμβρίου (3127)
- ► Σεπτεμβρίου (2173)
-
▼
Ιουλίου
(4578)
-
▼
Ιουλ 04
(164)
- Comparison of national ZP equations for lean meat ...
- Correlation of CD4 counts with clinical and histop...
- Health anxiety in dermatology
- Topical timolol for treatment of penile Kaposi sar...
- Riga-Fede disease in the upper jaw in an infant
- Superiority of a vitamin B12-barrier cream compare...
- Age-Associated Intracellular Superoxide Dismutase ...
- Neurological and psychiatric associations in bullo...
- Imiquimod-applied Interleukin-10 deficient mice be...
- Ultraviolet radiation and skin mast cells: Effects...
- A Rare Case of Primary Bilateral Adrenal Lymphoma
- Age-related changes in the morphological relations...
- Age-related changes in the morphological relations...
- Secondary encephalocele in infant following subdur...
- The effect of pneumococcal conjugate vaccines on i...
- Changes in pediatric tracheostomy tubes exposed to...
- Assessment of weight gain following adenotonsillec...
- Evaluation of ototoxicity of intratympanic adminis...
- In response to the letter to the editor regarding:...
- Letter to the Editor regarding: Rat model of chron...
- Anaesthesia for laryngo-tracheal surgery, includin...
- Regional anaesthesia for ophthalmic surgery
- Diagnosis and management of malignant hyperthermia
- Critical care management of pulmonary hypertension
- Postoperative management of the difficult airway
- Mepolizumab Efficacy in Patients with Severe Eosin...
- Reduced need for surgery in severe nasal polyposis...
- Reviewing the impact of lipid synthetic flux on Th...
- Surgical Treatment of Retrosternal Goitre
- Vitamin D supplementation in primary allergy preve...
- Estimating the causal effect of body mass index on...
- Disfunctionele mictie
- Surgical Treatment of Retrosternal Goitre
- A Double-Blind, Randomised Study Comparing the Ski...
- Specific immunotherapy in allergic rhinitis
- Rare presentation of intractable tuberculous panop...
- L'Union européenne comme acteur international
- Relations extérieures
- Ter Inleiding
- Middelburg en de Mediene: Joods leven in Zeeland d...
- Foliar absorption of pesticide in combination with...
- Insecticidal activity and composition of essential...
- Insecticide effect of zeolites on the tomato leafm...
- Distribution and evolution of the lectin family in...
- Microwave Spectroscopy of a Carbon Nanotube Charge...
- T-tubule remodelling disturbs localized β2-adrener...
- Investigation into the application of adult human ...
- Relationship between brainstem neurodegeneration a...
- Modulation of NMDA receptor activity by dopamine r...
- Exploratory study into the effect of abdominal mas...
- High-resolution magnetic resonance imaging reveals...
- Inhibition of Poxvirus Gene Expression and Genome ...
- What Have We Learned from Structural Models?
- Generalized Observational Slicing for Tree-Represe...
- Dicer Deficiency Differentially Impacts Microglia ...
- Biomarkers and Surrogate Endpoints in Uveitis: The...
- Modelling the upper atmosphere of the gas giant pl...
- Modulation of calcium-activated potassium currents...
- The growth of love
- Brown, B. (2015). Daring greatly: How the courage ...
- Commentary on the MID3 good practices paper
- Perspectives on love as a component of professiona...
- Photobacterium sanguinicancri sp. nov. isolated fr...
- The Employment of Servants in Fifteenth and Sixtee...
- De vele gezichten van de Gentse rechtsfaculteit (1...
- Production of lactic acid and derivatives from gra...
- Ribosome inactivating proteins from Rosaceae
- Anthropometric study of the Caucasian nose in the ...
- The strength of infinitary Ramseyan principles can...
- Ordinal notation systems corresponding to Friedman...
- Ackermannian Goodstein Principles for First Order ...
- The smallest galaxies: tests for cosmological and ...
- Paradoxical tensions when opting for a co-creation...
- Incidence, Treatment Patterns, and Health Care Cos...
- Thymus vulgaris L. extract has antimicrobial and a...
- Different expression patterns of Lin28 and Lin28b ...
- Role of fungi in chronic rhinosinusitis through IT...
- Occlusion handling framework for tracking in smart...
- "If only they had a file on every pupil". On the m...
- Love: Recognising relationships in work with vulne...
- Forecasted trends in disability and life expectanc...
- Aroha: 'Loving' within a statutory and bi-cultural...
- Editorial
- Faith, hope and love in social work practice
- Children's quest for love and professional child p...
- Orphan love in the age of capital
- Moderate alcohol consumption as risk factor for ad...
- Active dendritic integration as a mechanism for ro...
- Pregnancy incidence and outcomes in women with per...
- Cultivating human beings, not human dongs: Challen...
- Children with speech and language communication ne...
- Mind sets and IFL: texts and tasks. A theoretical ...
- Preparation of Nano- and Microstructures For Drug ...
- Do you love me? An empirical analysis of the feeli...
- The BLUEDISK survey: molecular gas distribution an...
- Fostering Restoration The impact of love and secon...
- Barnes, M., Brannelly, T., Ward, L., Ward, N. (201...
- Fenton, M. (2015). Social care and child welfare i...
- More than just a bracelet: the use of material sym...
- Phosphorylated ribosomal protein S6 correlation wi...
-
▼
Ιουλ 04
(164)
-
►
2016
(13807)
- ► Δεκεμβρίου (700)
- ► Σεπτεμβρίου (600)
- ► Φεβρουαρίου (1350)
- ► Ιανουαρίου (1400)
-
►
2015
(1500)
- ► Δεκεμβρίου (1450)
Ετικέτες
Τρίτη 4 Ιουλίου 2017
Ribosome inactivating proteins from Rosaceae
Εγγραφή σε:
Σχόλια ανάρτησης (Atom)
Δεν υπάρχουν σχόλια:
Δημοσίευση σχολίου