The coexistence of two blaNDM-5 genes on an IncF plasmid as revealed by nanopore sequencing [PublishAheadOfPrint]

In a carbapenem-resistant Escherichia coli clinical isolate of sequence type 167, two copies of bla_NDM-5 were found on a 144,225-bp IncF self-transmissible plasmid of the F36:A4:B- type. Both bla_NDM-5 genes were located in 11,065-bp regions flanked by two copies of IS26. The two regions were identical in sequence but were present at different locations on the plasmid, suggesting a duplication of the same region. This study highlights the complex genetic contexts of bla_NDM-5.

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