Αρχειοθήκη ιστολογίου

Πέμπτη 14 Φεβρουαρίου 2019

Decreased ciliary beat responsiveness to acetylcholine in the nasal polyp epithelium

Abstract

Objective

We investigated the difference in ciliary beat responsiveness to acetylcholine in ex vivo and the difference in the expressions of associated molecules (M1/M3 muscarinic receptors, pannexin‐1 and P2X7 purinergic receptor) between the nasal polyp and turbinate mucosa.

Study Design

Laboratorial study.

Participants

Nasal polyp and inferior turbinate were collected from patients with hypertrophic rhinitis and/or nasal polyp during endoscopic sinonasal surgery.

Main outcome measures

The mucosa was cut into thin strips, and ciliary movement was observed under a phase‐contrast light microscope equipped with a high‐speed digital video camera. The samples were also examined by scanning electron microscopy, fluorescence immunohistochemistry, and quantitative reverse transcription‐polymerase chain reaction.

Results

Cilia were well preserved in both tissues at the ultrastructural level. The baseline ciliary beat frequency (CBF) was not different between the two tissues. The CBF of the turbinate was significantly increased by stimulation with acetylcholine (P<0.001), but that of the polyp was not. The ratio of the acetylcholine‐stimulated CBF to the baseline CBF was significantly lower in the polyp than in the turbinate (P<0.001). Immunohistochemical study revealed that immunoreactivities for M3, pannexin‐1 and P2X7 were weaker in the polyp than in the turbinate. The mRNA expressions of M1, M3 and P2X7 were significantly lower and that of pannexin‐1 tended to be lower in the polyp than in the turbinate.

Conclusions

These results indicate that ciliary beat responsiveness to acetylcholine is decreased in the nasal polyp. This may be explained by the decreased expressions of M3, P2X7 and probably pannexin‐1 in this tissue.

Keywords

Nasal polyp, ciliary beat frequency, acetylcholine, muscarinic receptor, pannexin‐1 channel, P2X7 purinergic receptor

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